Posted by admin on May 31, 2016 in |
The present work was designed to perform the comparative study between the seeds of two selected plants i.e., Butea monosperma & Nigella sativa. The study includes phytochemical screening of primary and secondary metabolites, physico-chemical analysis, pharmacological studies, elemental analysis, and flouroscent studies. The comparative priliminary studies of Butea monosperma and Nigella sativa seeds in different solvent extracts shows the presence of primary metabolites such as carbohydrates, proteins, lipids and secondary metabolites such as Alkaloids, phenolic compounds, Flavonoides, Steroids, Tannins & saponins etc. In the preliminary screening of Nigella sativa ethanolic extract was more effective and shows the presence of maximum number of secondary metabolites, whereas In the preliminary screening of Butea monosperma methanolic extract was more effective and shows the presence of maximum number of secondary metabolites. The pharmacognostic studies was also carried out in order to check the adulteration and quality of crude drugs, which involves the evaluation of organoleptic characters such as colour, odour, taste and other macroscopical physical characters. The comparative physicochemical analysis of total ash...
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Posted by admin on May 31, 2016 in |
Patients with DM are often afflicted by debilitating urologic complications. The main aim of this study is to investigate the effect of streptozotocin (STZ)-induced type I diabetes on contractile responses to numerous stimuli, intracellular cation concentrations and morphology in the rat. Adult rats (n=12) were humanely killed and detrusor muscles of urinary bladder located and excised rapidly and placed in organ baths. Then urinary bladder detrusor muscles were blotted, weighed and dissolved in concentrated nitric acid for the measurements of cation contents. The diabetic rats had significantly (P<0.05) elevated blood glucose compared to control rats. The contractile responses induced by EFS were significantly (P<0.05) increased in the diabetic urinary bladder tissue in comparison to the control urinary bladder tissue. The increase in the contractile force was calcium-dependent in both control and diabetic urinary bladder tissues. The results also showed no significant differences in the levels of sodium, potassium, calcium, between the diabetic and control urinary bladder (p>0.05). However, there was a significant (P<0.05) decrease in magnesium in the diabetic...
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Posted by admin on May 30, 2015 in |
The present study deals with the evaluation of the antimicrobial activity in methanolic extracts of in vivo (leaf, stem and seed) and in vitro (callus) plant parts of Merremia dissecta and Merremia aegyptia belonging to family Convolvulaceae. The antimicrobial activity against six different microbial strains, four bacterial viz. Bacillus thuringiensis, Bacillus subtilis, Eschrechia coli and Pseudomonas putida and two fungal strains viz. Candida albicans and Aspergillus niger was studied. Initially, plant extracts prepared in four different solvents (Aqueous, Methanol, Ethanol, and Petroleum Ether) were screened for their antimicrobial activity through TTZ test (Tetrazolium Chloride Test). It was assessed that alcoholic sample extracts possessed highest antimicrobial activity amongst all. Later the antimicrobial activity of the methanolic extracts was confirmed using Disc-Agar Diffusion technique. The inhibition zone observed was representative of the antimicrobial potency of that particular sample extract. Zones of inhibition ranging from 6mm to 14mm were observed. The largest zones of inhibition were observed from the leaf extracts of these plants and the leaf extract of Merremia aegyptia also...
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Posted by admin on May 30, 2015 in |
In the present study the anti-arthritic activity of ethanolic extracts of leaf and seed of Elaeocarpus serratus L. was evaluated in vitro using two models namely on the inhibition of protein denaturation and anti-proteinase activities. Indomethacin was used as a standard drug. Results revealed that the ethanolic leaf and seed extracts of E. serratus at two different concentrations (200 and 400 µg/ml) possessed notable activity as compared to the standard drug indomethacin, justifying its usefulness in the treatment of inflammation associated diseases like...
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Posted by admin on May 30, 2015 in |
Diaporthephaseolorum is endophytic fungi isolated from Jatitree (Tectonagrandis L.f). Endophytic microbes were microbes that spent part or all of their life live within plant tissue of a host plant. They produce secondary metabolites with potent anti microbial activity. Jatitree (Tectonagrandis L.f) was one of herbal plants that act as host plants for endophytic microbes that produce secondary metabolites with anti-microbial activity. The current study focussed on isolation of the endophytic microbes from branches of Jati tree and their secondary metabolites. Isolation of endophytes were performed in PDY (potato dextrose yeast) and further extracted using EtoAC. Four fractions (F I – F IV) was obtained from EtOAc extract through column chromatography (n-Hex: EtoAc, ratio 50:1). Further, agar diffusion method was performed to assess their anti-microbial activity. Results demonstrated that F IV has potent towards Staphylococcus aureus (11.10mm). Its GC-MS profile strongly indicated the presence of fatty acid and phenolic related compound. In conclusion, secondary metabolites of endohpytes isolated from Jati tree branches contained predominantly phenol and fatty acid related compound...
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