Posted by admin on Sep 1, 2014 in |
The present study deals with the phytochemical examination of therapeutic importance of Tiliacora acuminata (Lan) Hook. F & Thomas an important medicinal plant. Qualitative phytochemical analysis of the methanol and ethanol extracts prepared from Tiliacora acuminata stem and leaf revealed the presence of alkaloids, anthraquinones, catechins, coumarins, flavonoids, phenols, quinones, saponins, steroids, sugar, glycosides, tannins, and xanthoproteins. The FT-IR spectrum confirmed the presence of alkyl group, methyl group, alcohol group, ethers, esters, carboxylic acid, and anhydrides. The bioactive components of the ethanol extract of Tiliacora acuminata stem and leaf were investigated using Perkin-Elmer Gas Chromatography-Mass Spectrometry (GC-MS), while the mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology (NIST) library. GC-MS analysis of ethanol extract of stem of Tiliacora acuminata revealed the existence of 9,12,15-Octadecatrienoic acid (Z,Z,Z) (85.6%), 9,12,15,Octadecadienoic acid (Z,Z) (7.2%), Asarone (3.5%), Hexa-decanoic acid, ethyl ester (1.6%), Methyl-α-d-ribofuranoside (1.4%) and 9,12,15,Octadecadienoic acid, methyl ester (E,E)- (0.8%) and leaf of Tiliacora acuminata revealed the existence of 3,7,11,15-Tetramethyl-2-hexadecen-1-ol (14.9%),...
Read More
Posted by admin on Sep 1, 2014 in |
Menopause is a stage which every woman has to pass through. Menopausal stage brings physical changes as well as microbial changes in the oral cavity. The latter change is often left unnoticed, or it is neglected. The important feature during menopause is a rapid decline in the level of the hormone estrogen. Due to the gradual to a rapid drop in the level of the hormone, there is a lower absorption of the dietary calcium. This change will lead to a weaker bone or osteoporosis. Osteoporosis is seen in the alveolar bone, especially in the mandible area. Pockets are formed in the Periodontium (tissues and bone surrounding the tooth). These pockets will help the anaerobic bacteria like Prevotella, Porphyromonas gingivalis, Fusobacterium, and the other anaerobic bacteria to multiply and harbor leading to tissue damage and tooth loss. A comparative study was done between Premenopausal and Postmenopausal women. One hundred ten subjects were from each group was chosen for the study. Periodontal bacteria were isolated. Statistical analysis was done with...
Read More
Posted by admin on Sep 1, 2014 in |
Phytochemicals with mosquitocidal potential are now recognized as potent alternative insecticides. This study aims at exploring the mosquitocidal potential of the extract of the medicinal plant Sphaeranthus indicus. The soxhlet extracts of flowers, leaves and aerial parts of S. indicus with four solvents viz., hexane, chloroform, ethyl acetate, and methanol were screened against the larvae of the vector mosquitoes Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus. The commercially available synthetic insecticide temephos was used as positive control. The LC50 values for the hexane extract of the flowers, leaves and aerial parts were 75.62, 48.22, 70.23 mg/L; 18.61, 53.34, 33.04 mg/L and 191.9, 71.58, 116.21 mg/L respectively for A. aegypti, A. stephensi and C. quinquefasciatus. Bioassay-guided fractionation was carried out for the hexane extract of the leaves of S. indicus using a silica gel column with eluents hexane followed by 5% and 10% ethyl acetate in hexane. Fractions were examined by thin layer chromatography (TLC) using silica gel, 60 F254 plates using hexane-ethyl acetate (95:5), pooled the similar fractions and...
Read More
Posted by admin on Sep 1, 2014 in |
Kidney stones are a global affliction causing a great deal of morbidity and economic loss. The prevalence and control of nephrolithiasis are very important. Herbs and herbal drugs have created interest among the people by their clinically proven effects like immunomodulation, adaptogenic, and antimutagenic. Also, the overuse of synthetic drugs, which results in a higher incidence of adverse drug reactions, has motivated humans to return to nature for safe remedies. The problem of kidney stones or calculi has been a great concern since ancient times and many remedies have been employed. During the ages, these stones are found in all parts of the urinary tract, the kidney, the ureters, and the urinary bladder and may vary considerably in size. In the present investigation, an attempt has been made to emphasis on the studies like in-vitro Biomineralization, Scanning Electron Microscope (SEM) t-RNA sequencing and an herbal option for kidney stone problem based on the promising results obtained in the present...
Read More
Posted by admin on Sep 1, 2014 in |
Objective: To evaluate the curative effect of Elephantopus scaber Linn. on N’Nitrosodiethylamine induced hepatocellular carcinoma in experimental rats. Methods: Phytochemical analysis of the different solvent extracts of E. scaber revealed various constituents such as flavonoids, terpenoids, phenols, etc. in the methanolic extract, which was selected for further study. The curative ability of two different doses of E. scaber methanolic extracts (100mg/kg and 200mg/kg) was evaluated against NDEA (0.02% in water) induced liver cancer in male Wistar rats. Quantification of serum AST, ALT, ALP, and LDH was conducted to assess the extent of hepatotoxicity. Estimation of Reduced Glutathione (GSH), Glutathione-S-Transferase (GST), Glutathione Peroxidase (GPx), Catalase (CAT) and Malondialdehyde (MDA) determined the anti-oxidant activity of the plant. Histopathological examination and immunohistochemical studies for cancer markers namely Proliferating Cell Nuclear Antigen (PCNA), vascular endothelial Growth Factor (VEGF) and Cyclin D1 were conducted on liver tissues to confirm the anti-cancer properties of the plant extract. Results: Elephantopus scaber methanolic extract significantly (p≤0.05) reversed the elevation of serum AST, ALT, ALP, LDH, and tissue...
Read More
