Posted by admin on Sep 1, 2013 in |
Allium sativum linn (Garlic) belong to family Liliaceae has been widely known since ancient times for medicinal use and for flavouring food. It contains large amount of sulphur containing compounds like Alliin, Allicin, Ajoene, Diallyl sulphide, Diallyl disulphide Diallyl trisulfide, Vinyldithiines which has beneficial activity including Antithrombotic, Anticancer, Antiviral, Antifungal and Antimicrobial, Anti-inflammatory etc. Ajoene is known as an inhibitor of platelet aggregation induced by all known agonists which also a good inhibitor of tumour and antifungal property. The present study deals with pharmacognostical parameters for bulb of Allium sativum which mainly consist of the detailed macromorphology of cloves and bulb, microscopy and micrometry of transverse section of cloves of Allium sativum linn which has been carried out by using MOTIC Digital Microscope. The other parameters like physicochemical constant and phytochemical screening were studied. The study will provide referential information for the correct identification and standardization of crude...
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Posted by admin on Sep 1, 2013 in |
Aim: To compare the immunomodulatory effect of Ocimum sanctum and Ocimum basilicum in mice. Methods: Acute oral toxicity test was performed as per OECD -23 guidelines. Maximum dose level of 2000 mg/kg was selected for toxicological evaluation. Delayed type hypersensitivity reaction were carried out for assessment of cell mediated immune functions Result: Administration of Ocimum sanctum and Ocimum basilicum did not produced any mortality or any signs of behavioral changes or toxicity at the dose level of 2000 mg/kg body weight in mice. The result of the present study showed that acute administration of extract of Ocimum sanctum and Ocimum basilicum may be safe as the LD50 may be greater than 2000mg/kg. Treatment with Ocimum sanctum enhanced DTH reaction, which is reflected from foot pad thickness when compared with Ocimum basilicum and control groups whereas a similar effect was observed when compared to the reference drug Aswagandha choorna, which is a well-known immune booster. Conclusion: Increase in DTH reaction in mice in response in T cell dependent antigen revealed...
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Posted by admin on Sep 1, 2013 in |
Overlapping genes are pairs of adjacent genes whose coding regions are partially or entirely overlapping. Overlapping genes in prokaryotes are a means to minimize genome size and increase the density of genetic information. Overlapping genes play an important role in genome reduction of Mycoplasmas during the course of their degenerative evolution. In this study overlapping genes in the genomes of two obligatory human parasites, Mycoplasma hominis and Mycoplasma penetrans were extracted and systematically analyzed. Overlapping genes were classified into different categories based on their direction of transcription. Gene pairs that occur as overlapping in both the genomes, gene pairs that occur as overlapping in one genome but are split in the other genome were separated out and more closely analysed. The study revealed that most of the overlapping genes in these genomes are formed due to loss of a stop codon or frame shift. The present study also emphasizes the significance of overlapping genes in the evolution of these...
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Posted by admin on Sep 1, 2013 in |
The degradation pathway of sitagliptin in bulk and tablet has been investigated during stress study. Major degradation products were isolated in pure form and characterized using mass and NMR spectroscopy. Three previously unreported impurities were found to be, 3-(trifluoromethyl)-6, 7-dihydro[1,2,4]triazolo[4, 3-a]pyrazin-8(5H)-one, (2E)-1-[3-(trifluoromethyl)-5, 6-dihydro[1, 2, 4]triazolo[4, 3-a]pyrazin-7(8H)-yl]-4-(2, 4, 5-trifluorophenyl)but-2-en-1-one and (3E)-1-[3-(trifluoromethyl)-5,6-dihydro[1,2,4]triazolo[4, 3-a]pyrazin-7(8H)-yl]-4-(2, 4, 5-trifluorophenyl)but-3-en-1-one. Further, a stability-indicating reverse phase HPLC assay method was developed on Poroshell 120 EC-C18 (3X150mm, 2.7µ) column using mobile phase consisting of 5mM ammonium acetate and acetonitrile with gradient elution in presence of spiked degradation products and impurity. The flow rate was 0.5ml/min and detection was at 210nm. The method was found to be linear over 10µg-500µg/ml (r2>0.999). The method was validated with respect to accuracy, precision, specificity, robustness and found to be stability...
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Posted by admin on Sep 1, 2013 in |
The present investigation deals with development of fast dissolving tablets of Domperidone to produce the intended benefits. Domperidone is an antiemetic and a prokinetic medicine. Domperidone is water insoluble drug with problems of variable bioavailability and bio-inequivalence. As precision of dosing and patient’s compliance become important prerequisite for quick relief from emesis, there is a need to develop a formulation for this drug which overcomes problems such as difficulty in swallowing, inconvenience in administration while traveling and better compliance. Hence, the present research work was held to develop a fast dissolving tablet of domperidone using Plantago ovata mucilage as a natural super disintegrant. The tablets were prepared by Direct Compression using microcrystalline cellulose as a directly compressible vehicle. Tablets were evaluated for their physicochemical properties and In-vitro dissolution study. The evaluation studies were performed such as Weight Variation, Thickness, Hardness, Disintegrating Time, Wetting Time, and In-vitro Drug release study. The Disintegration time of Fast Dissolving tablets were increased by the addition of concentration of Super...
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