Posted by admin on Sep 1, 2012 in |
Trypsin inhibitor (AATI) was purified from the seeds of Achyranthes aspera to homogeneity by conventional methods and its antimicrobial activity was tested on selected pathogenic microbes. The inhibitor significantly affected the growth of Proteus vulgaris followed by Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Klebsiella pneumonia with zones of inhibition recorded as 28mm, 26mm, 25mm, 20mm and 14mm respectively. No inhibition, however, was observed with Staphylococcus pneumonia strain. Minimum inhibitory concentration (MIC) values of AATI supported that Proteus vulgaris and Bacillus subtilis were the most sensitive strains against the inhibitor. AATI did not differentiate gram positive and gram negative bacteria in its antibacterial activity. The trypsin inhibitor was ineffective against fungal strains Asperigillus niger, Fusarium oxysporum, Alternaria alternate and Candida albicans...
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Posted by admin on Sep 1, 2012 in |
Thiazolidinediones (TZDs) were widely used for the treatment of type 2 diabetes. Recent studies have shown that TZDs have paradoxical effects on cardiovascular diseases. The objective of the present study was to investigate the effect of TZDs (Rosiglitazone and Pioglitazone) in High Fructose Diet (HFD) induced hypertension in rats. HFD was given for 14 weeks. After 8 weeks of hypertension induction period, treatment phase was started with Rosiglitazone (ROSI 10 and 30 mg/kg, p.o.) and Pioglitazone (PIO 10 and 30 mg/kg, p.o.) to the respective groups which were continued till 6 weeks. Systolic Blood Pressure (SBP) was measured weekly and serum glucose, triglyceride, cholesterol and HDL-C were measured at the end of study period. In HFD fed rats hypertension was observed after 8 weeks. Treatment with the test drugs significantly reversed the changes in serum enzyme levels as well as SBP made by HFD feeding compared to the control group. The study concludes that TZDs possess antihypertensive effect as exhibited in the present experimental...
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Posted by admin on Sep 1, 2012 in |
6-chloro 2, 4-diamino pyrimidine reacts with various aromatic aldehyde. Finally, the product was characterized by conventional and instrumental methods. Their structures were determined and important therapeutic properties were...
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Posted by admin on Sep 1, 2012 in |
Lipid-based drug delivery systems in the form of triglyceride emulsions, micellar systems and liposomes have been used for parenteral administration for the last few decades. Large number of new chemical entities (NCE) presents formulation and bioavailability problems because of the dose and poor solubility in solvent and co-solvent systems. In addition, high drug concentrations can lead to irritation and pain at the site of injection. There is an increasing interest to expand the range of targetable lipid based systems to solubilize a wide variety of drugs, to improve stability, ease of processing and manufacture in a sterile form. New class of parenteral lipid based drug delivery system includes Tocol emulsions, solid lipid nanoparticles and nanosuspensions, sterically stabilized phospholipid micelles, lipid micro-bubbles and lipoprotein drug carriers. This review article covers the challenges faced by the formulation scientist at each stage of product development of lipid based drug delivery...
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Posted by admin on Sep 1, 2012 in |
The present study was undertaken to investigate in-vitro antioxidant activity of aqueous and hydroalcoholic extract of whole plant of Bacopa monnieri Linn. Family- Scrophularaceae. The total Phenolic content was determined using folin ciocalteau method while the total flavonoid content was determined using aluminium chloride method. In vitro antioxidant activity was evaluated using the Reducing power assay, Hydrogen peroxide scavenging assay, nitric oxide scavenging activity, superoxide scavenging activity and hydroxyl radical scavenging activity. The hydroalcoholic extract had more phenol concentration (116.1 mg/g of extract) when compared to aqueous extract (58 mg/g of extract). The flavonoid content was more in hydroalcoholic extract (242.6 mg/g of extract) when compared to that of aqueous extract (202.8 mg/g of extract). The reducing power and hydrogen peroxide scavenging of the extract was found to be concentration dependent. The nitric oxide scavenging activity, superoxide scavenging activity and Hydroxyl radical scavenging activity was also concentration dependent with IC50 value being 254.70 µg/ml , 934.06 µg/ml and 510.60 µg/ml respectively for Aqueous extract and 169.22 µg/ml, 495.83 µg/ml,...
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