Posted by admin on Aug 31, 2021 in |
In this present study, the objective was to extract crude saponins from the root of Phoenix pusilla (PP) and to check its inhibitory potential of α- amylase and α- glucosidase enzyme. Saponins were isolated by partitioning between butanol and water. Further, the presence of saponin was confirmed by the foam test. Then the crude saponins were analyzed by UV- spectroscopy in the range of 200-400 nm and Fourier transform infrared spectroscopy (FTIR). FTIR results revealed the presence of – OH, -C-H, C=O functional groups. In-vitro antidiabetic activity assay of isolated crude saponin with acarbose as positive standard showed efficient hypoglycemic ability. Saponins have application in the food industry, cosmetics preparation and also exhibit various pharmaceutical activities. So plant-based saponin isolation in the pure form will be a boon to human utilization. This was the first report of in vitro antidiabetic potential of crude saponins of PP...
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Posted by admin on Aug 31, 2021 in |
The aim of the present study was to improve the release rate of herbal drugs by microsponges prepared through quasi-emulsion solvent diffusion technique using ethyl cellulose and PVA as carriers. The microsponges were characterized by FTIR, DSC and SEM studies followed by the determination of total drug content and entrapment efficiency. Anti-arthritic activity was evaluated by Freund’s Complete Adjuvant (FCA) induced arthritic rat model. RA was induced by intradermal injection of 0.1 ml FCA in the left hind paw. The animals were treated with doses 50:50, 70:30, 30:70, and 100:100 mg/kg b.w. of microsponge formulation. Methotrexate at a dose of 10 mg/kg is used as a standard drug. Assessment of paw volume, % inhibition of paw volume, serum parameters for RA factor determination and histopathological examination was also carried out.In this current study, the microsponge formulation (B. serrata + A. barbadensis) had a better effect on controlling CFA induced arthritis. Formulation codes M1 and M4 showed a good reduction in paw edema and joint thickness. It produced a significant...
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Posted by admin on Aug 31, 2021 in |
Exposure to heavy metals may modify fish behavior, metabolism, physiology, growth, and reproduction. They are also responsible for introducing genetic and teratogenic effects in fish. Now a day’s in many regions; fish production is decreased due to the occurrence of diseases caused by different pathogens in aquaculture. The increasing level of trace elements is dangerous for fish growth and reproduction and for human beings. Rapid industrialization is contaminating natural freshwater by heavy metals (Fe, Zn, Co, Pb, Ni, Cr and Mn), turning it into a global problem. The effect of pollution on the aquatic ecosystem of river Ganga was monitored, and a field study was conducted by analyzing the different biomarkers enzymes. Biochemical parameters in the various tissues (muscles, liver, gills, and kidney) of the Indian carp Cirrhinusreba collected from the river Ganga from different study sites of Kanpur. The catalase peroxides, hydrogen peroxides, and protein content level significantly increased as compared upstream to downstream. The catalase activity in the tissue of carp fish was found to be significantly...
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Posted by admin on Aug 31, 2021 in |
Microorganisms that live their life in places with high salt concentrations are known as Halophiles. Halophilic bacteria play a majority role in hypersaline environments than other microorganisms. These Halophiles are having intense strategies of adaptation and tolerate them to sustain their survival in the saline extreme, such adaptation in hypersaline environments makes them to give unique attention to the researcher for their biological properties. The diversity of halophiles bacteria incorporates aerobic and anaerobic phototrophs, aerobic heterotrophs, fermenters, denitrifies, sulfate reducers, and methanogens. The biotechnological important of the halophilic bacteria, such as producing a Novel bioactive compounds, industrially important enzyme, Bio-surfactants, Stabilizers Exopolysacharides, Probiotics etc. Secondary metabolites are organic compounds produced by bacteria, fungi, or plants which are not directly involved in the normal growth, development, or reproduction of the organism. Some common examples of secondary metabolites include ergot alkaloids, antibiotics, naphthalenes, nucleosides, phenazines, quinolines, terpenoids, peptides, and growth...
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Posted by admin on Aug 31, 2021 in |
The earlier reports on Nardostachys jatamansi show contain a various class of chemical constituents that have influenced the anti-inflammatory and anti-candidal activities. The present study reports the evaluation of anti-inflammatory and anti-candidal activities of N. jatamansi and also studies pharmacokinetic properties using in-silico model. In ongoing research work, we used individual extracts of dichloromethane, ethyl acetate, methanol, and water were prepared. The anti-inflammatory activity of different solvent extracts was carried out using gelatin zymography through detection of MMP-2 and MMP-9 and on other side anti-candidal activity was carried out using Nitro blue tetrazolium assay (NBT). Bioactivity and pharmacokinetic parameters of all extracts were calculated using the property calculation online toolkit. From the results, it is observed that, among all the tested extracts, ethyl acetate extract was found to be more potent towards phagocytosis (more than 5 MPN) and Candida albicans (41%) at 100 µg/mL while dichloromethane extract possesses profound anti-inflammatory activity towards MMP-2 (90%) and MMP-9 (85%) as compared to standardtetracyline HCl (positive control) followed by other extracts. Desoxo-Narchinol...
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