Posted by admin on Aug 31, 2021 in |
Anisomeles species are traditionally used for treating aliments like hysteria, amentia, dyspepsia, epilepsy, anorexia, diarrhoea etc. the present work, we aimed to evaluate the anti-diabetic activity and hepatoprotective activities of aqueous and ethanolic extracts of Anisomeles malabarica roots. Anti-diabetic activity was studied at three dose levels 100, 200, 400 mg/kg of aqueous and ethanolic extracts in normoglycemic and Streptozotocin (STZ) induced diabetic rat models. Tolbutamide (40 mg/kg) was used as standard treatment. Blood glucose levels were monitored at 1, 2, 4, 8, 16 and 24 h post-administration using glucometer. The hepatoprotective activity of the extracts was assessed at dose levels 100, 200, 400 mg/kg of aqueous and ethanolic extracts in ethanol intoxicated groups. Silymarin (100 mg/kg) was used as Levels of ALT, AST, ALP, and Bilirubin were measured using standard kits. Liver volumes and liver weights of the animals were also recorded. The results revealed that both aqueous and ethanolic extracts have anti-diabetic and hepatoprotective activities; however, the activity was more prominent with 400 mg/kg of ethanolic extracts and...
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Posted by admin on Aug 31, 2021 in |
Pashanabheda is used as antiurolithiatic in Ayurveda. In the present study, Punica granatum is used for screening in-vitro antiurolithiatic potentials. Screening of in-vitro antiurolithiatic potentials of aqueous leaf extract of Punica granatum. The extract was subjected to Preliminary phytochemical analysis. In-vitro anti-urolithiatic activity was screened by nucleation and aggregation assay in which the formation and growth of CaOx crystals were quantified. The extract exhibited inhibitory action in both nucleation and aggregation assays to a significant level. In the aggregation assay gradual decrease in the CaOx crystal nucleation, as well as growth, was observed by light microscopy. The findings of the nucleation assay indicate that the extract inhibited the crystallization of CaOx in the solution. There were less and smaller particles with increasing concentration of the extract. The increasing concentrations of extract (100, 200, 300, 400 and 500 µg/ml) inhibited the CaOx crystal growth. Punica granatum extract demonstrated slightly better results compared to Cystone standard solution to inhibit the formation of calcium oxalate dihydrate crystals in the nucleation assay. The...
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Posted by admin on Aug 31, 2021 in |
Two-dimensional (2D) culture systems are indispensably used as in-vitro tools to improve our perception and understanding of cell biology, tissue engineering, tissue morphology, mechanisms of diseases, and drug action. However, the conventional 2D culture systems suffer from limitations such as alterations in cell morphology and lack of interactions between extracellular and cellular environments. As a result, three-dimensional (3D) cell cultures have received remarkable attention in studies such as drug discovery and development; the sandwich model being one of the unique cellular models widely used for analyzing tissue metabolism and functions. In this study, we devised a novel Gel Casting Tool (GCT) for in-vitro toxicity analysis. Liver cells need complex 3D micro-architecture to express tissue-specific functions and were suitable for this study. Human Carcinoma Cell line (HepG2 cells) with Gelatin methacrylamide (GelMA) hydrogel was used to check the efficacy of the GCT. The results showed that GCT Sandwiched HepG2 exhibited high cell viability, proliferation, and tissue-specific functions such as albumin and urea over 7 days. The major advantage of this...
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Posted by admin on Aug 31, 2021 in |
Most of the Bio-medical wastes got dumped in highly eminent aquatic sources. To control this pollution due to biomedical wastes, the current study focused on the development of hydrogel bead nanocomposite incorporated with the adsorbent and nanoparticles along with biopolymer. The formed hydrogel bead nanocomposite acts as an effective adsorbent of infectious microbes, heavy metals and pollution causing materials. The conjugation efficiency of the activated tea dust, zinc oxide nanoparticles, and bioconjugated system was analyzed using UV-Vis spectroscopy which shows the maximum absorption at 330nm, 379nm, and 580nm in the conjugation system. Further, this was analyzed using FTIR shows good functional groups stretching, Dynamic Light Scattering analysis depicts the particle size as 5.203µm and has good stability. The effectiveness of the hydrogel bead nanocomposite on the wastewater sample was characterized through solubility test and heavy metal adsorption ability. The bead does not solubilize in the water sample over a long time which shows the high stability of the conjugated system. Whereas the adsorption efficiency (%R) of the hydrogel bead...
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Posted by admin on Aug 31, 2021 in |
Reverse Phase High-Performance Liquid chromatography is a widely and commonly used analytical method for qualitative and quantitative analysis. A simple, accurate, economical, rapid, and precise RP-HPLC process has been proposed and validated for the determination of esomeprazole in immense bulk and dispensed pharmaceutical drug form. The RP-HPLC separation was achieved on Symmetry ODS RP C18, 5 µm, 15 mm × 4.6 mm i.d. column using mobile phase Phosphate buffer (pH=3.6): ACN = 50:50 pH Adjudged with Orthophosphoric acid at continuance effusion of 1.0 ml/min at ambient temperature. The retention times were observed at 3.797 min for esomeprazole. The calibration plot is linear beyond the concentration range 0-50 μg/ml for esomeprazole. Quantification was achieved with UV detection at 246 nm over the Beer-Lambert’s range. The proposed methodology is validated statistically as per the ICH guidelines for various validation parameters includes, including Accuracy, Precision, Linearity and range, Limit of detection, and Limit of Quantification. The above method is developed and validated successfully for the routine quantitative analysis of esomeprazole in bulk...
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