Posted by admin on Aug 31, 2021 in |
Covid-19, caused by Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2) is a life-threatening disease that has been declared a global pandemic. Even with the intervention of numerous medical systems to combat Covid-19, an effective remedy is not yet in sight rather than supportive therapies accompanied with anti-viral drugs and vaccines. The usage of these medicines has caused for unexpected side effects, and at present, various mutant forms of this virus have been identified from different countries. Hence, the necessity of an effective treatment regimen is highly expected. The attention direct towards Traditional Medicine (TM) is increasing day by day as it is well known for immune modulation. Therefore, this review was conducted with the aim of systematically reviewing the relevant literature of TM based on clinical studies conducted on Covid-19 patients and provides a summary on the evidence-based potential use of TM in the treatment of Covid-19. This review was conducted by using the databases; Science direct and PubMed for studies published from 1st of December 2019 to...
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Posted by admin on Aug 31, 2021 in |
In the present study, synthesis of silver nanoparticles (AgNPs) using leaves and stem bark of Pterocarpus indicus were tested against human breast cancer cell line (MCF-7) separately. The synthesized AgNPs were characterized using UV-visible spectroscopy, X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Scanning Electron Microscope (SEM) and zeta potential. The synthesized silver nanoparticles were checked with the color variation, and it was confirmed by UV-vis spectral analysis. The morphology of the synthesized nanoparticles was analyzed using SEM. The XRD was done to find out the crystalline structure of the compound. FTIR measurements are carried out to identify the possible biomolecules responsible for capping and efficient stabilization of the silver NPs synthesized using Pterocarpus indicus. The synthesized leaves-AgNPs and stem bark-AgNPs of Pterocarpus indicus were tested against the MCF-7 cell line to determine cell viability. Results showed that the stem bark-AgNPs shows more stability than leaves-AgNPs due to their higher negative zeta potential (-16.3 mV) than nanoparticles biosynthesized from leaves-AgNPs (−13.1 mV). The synthesized silver nanoparticles inhibited the proliferation...
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Posted by admin on Aug 31, 2021 in |
The synthetic pyrethroids used in agriculture also absorb rapidly via different routes and slowly accumulate into various vital tissues and cause adverse effects on the health status of animals. It leads to produce a number of biochemical changes at the level of nucleic acids. The freshwater fish Ctenopharyn godonidella was exposed to sublethal concentrations (1/10th of LC50 value) of λ- cyhalothrin 5% EC for a period of 1, 4, 8 and 12 days to study the toxic impacts on DNA and RNA contents in various organs such as gill, muscle liver-kidney, and brain. In control fish, DNA content was in the order of Muscle (35.78)> Liver (34.17) > Kidney (28.74) > Brain (25.60) > Gill (18.71) and RNA was in the order of Kidney (37.15) > Muscle (36.45) > Liver (32.68) > Brain (28.15) > Gill (22.56). In the present study under sublethal concentrations of λ- cyhalothrin on day 12the percent depletion of DNA content in the test tissues were in the order of: Brain (21.43) > Gill (16.36)...
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Posted by admin on Aug 31, 2020 in |
The objective was to develop and validate an easy, economical, fast, reliable, reproducible, precise and accurate reversed-phase high-performance liquid chromatography (RP-HPLC) method for the estimation of tapentadol hydrochloride (TAP, a mu opioid-receptor agonist, and noradrenaline reuptake inhibitor) in the bulk and pharmaceutical dosage form. The chromatographic separation was achieved by using the HPLC system equipped with a C-18(2) column (250 × 4.6 mm, Particle size 5µm) at wavelength 217 nm. The mobile phase consisting of Potassium phosphate buffer: Acetonitrile (50:50 v/v) was used in isocratic mode. The flow rate was fixed at 1.0 mL/min with a continuous run up to 5 min, while the retention time was located near about 2.5 min. In the concentration range of 60-160 µgmL-1, the detector response was found linear with linear regressed equation Y=22791X+120122. In the assay of TAP, 99.65% of the drug was recovered. This method proved a satisfactory validation for all the parameters such as accuracy, linearity, specificity, precision, range, ruggedness, robustness, reproducibility, and peak purity assessment as per ICH guidelines. The results...
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Posted by admin on Aug 31, 2020 in |
Kutki (Picrorrhiza kurroa) is a medicinal herb with remarkable pharmacological properties. However, poor solubility of the active principles limits its medicinal value. This study sought to prepare kutki phospholipid complex in phospholipids such as phosphatidyl choline in order to improve its solubility and permeability. Kutki phospholipid complex (phytosome) was prepared by solvent evaporation method. The study was conducted using central composite design (CCD) with a 33 designs and total of 20 experimental runs. The complex was characterized for solubility, entrapment efficiency, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), particle size and zeta potential, Differential scanning calorimetry (DSC), X-ray diffraction (XRD), drug release, and in-vitro antioxidant activity. The optimized batch showed substantial enhancement of solubility and entrapment efficiency. FTIR study indicates the interaction between phosphatidylcholine and Kutki extract. SEM showed the surface morphology of complex depicting the conversion of crystalline drug into amorphous complex. The in-vitro release was significantly higher for the complex (93.43%) than the drug (19.65%) after 7 h. Enhanced solubility, absorption and antioxidant effect may...
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