Posted by admin on Aug 31, 2016 in |
In some disease like thalassemia major long-term transfusion alone inexorably produces the clinical problem of iron overload. Danshen and Milk thistle has been widely prescribed in traditional folk medicine for treatment of liver diseases. In this study we selected eight medicinal plants whose efficiencies had been previously demonstrated in treatment of liver diseases in ancient medicine were studied for their in vitro chelating activity. After preliminary evaluation of these plants in vitro condition, two most efficient plants were nominated for further evaluation in in vivo condition on iron-overloaded mice. The extracts with high iron chelation activity were injected intraperitoneally. Liver sections were stained by haematoxylin and eosin and Perls’ stain. Danshen and Milk thistle had maximum iron chelation activity at in vitro condition. The iron-overloaded mice treated with the extracts showed a significant decrease in plasma iron level and in plasma Fe3+ content. Perl̕ stain improved sensitivity of the test significantly in order to determine the low amount of deposited iron in the liver of iron- overloaded mice treated...
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Posted by admin on Aug 31, 2016 in |
Inula racemosa, Bombax ceiba and Allium sativum are important class of drug known as “Rasayana”. The Polysaccharide fraction of all the three Drugs was isolated from hot water extraction yielding about 50-60% of fructan fraction (Polysaccharide). The polysaccharide fraction was evaluated for immunomodulatory activity by antibody titer and cellular immune response. The immunosuppressant drug azathioprine (100 mg/Kg/body weight) was used in order to evaluate the potency of immunomodulation of fructan fraction of rasayana drugs. The toxicological studies was made and a dose of 1000mg/Kg for Inula racemosa and Bombax ceiba and 1200mg/kg in case of Allium sativum was found to be not toxic. The non specific immunity determined by survival rate against Candida albicans infection was improved after treatment with fructan fraction. An increase in delayed type hypersensitivity response (DTH), % neutrophil adhesion and in vivo phagocytosis by carbon clearance method was observed after treatment with fructan fraction. The Immunostimulant activity of fructan fraction in dose of 200mg/kg of body weight was found to be more effective than 100mg/kg...
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Posted by admin on Aug 31, 2016 in |
A simple, accurate, precise, rapid and economical RP-HPLC method was developed for the estimation of Cefpodoxime Proxetil (CPD) and Levofloxacin hemihydrate (LVX) in tablet dosage form. In Agilent – 1100/1200 Series, using C18 (250 x 4.6 mm x 5μ) column and with mobile phase composition of Phosphate buffer: Acetonitrile (pH 6.0) (50:50 v/v) at a flow rate of 1 ml/min was used. Detection was carried out at 240 nm. Retention time of CPD and LVX was found to be 5.64 & 5.97 min and 2.79 min, respectively. The method has been validated for linearity, accuracy and precision, LOD, LOQ and System suitability according to ICH Q2R1...
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Posted by admin on Aug 31, 2016 in |
Let G=(V,E) be a simple connected molecular graph in chemical graph theory, where the vertex set and edge set of G denoted by V(G) and E(G) respectively and its vertices correspond to the atoms and the edges correspond to the bonds. A topological index of a graph G is a numeric quantity related to G which is invariant under automorphisms of G. In this paper, the vertex Szeged index of Titania carbon Nanotubes TiO2(m,n) is...
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Posted by admin on Aug 31, 2016 in |
Male Wistar rats were procured from ‘The Bombay Veterinary College’, Parel, Mumbai. The animals were maintained and housed in cages and were acclimatized in laboratory conditions for ten days prior to the experiment. The experimental rats were fed orally with isoniazid 300 mg/ 70 kg body weight / day; rifampicin 450 mg / 70 kg body weight / day and Aloe vera extract 50 mg/ kg body weight / day for 30 days. Blood samples of the above groups (A to H) were taken after 30th day from cardiac puncture for estimation of mean concentration of complete blood Count. The present changes resulted from the antitubercular drugs in the hematological changes might be attributed to the toxic metabolites of the isoniazid and rifampicin. The toxic, reactive metabolites of the drugs bind to cellular macromolecules and release or form toxic free radicals intern caused the tissue damage. The present study was concluded that Supplementation of Aloe vera extract resulted in significant improvement in the hematological parameters might be due to...
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