Posted by admin on Aug 31, 2015 in |
Sildenafil, the active ingredient in Viagra (Pfizer), is a prescription medicine used for erectile dysfunction. Sildenafil were isolated and purified during the analysis of some herbal products marketed for treatment of erectile dysfunction. There is no standardization technique for herbal formulations hence the addition of adulterants in herbal is very difficult to get hold of. Most of the adulterants added are to enhance the efficiency of the preparation. Not only sildenafil but various other drugs such as Tedanafil, Verdinafil and their analogues were used in the formulations. We have extracted sildenafil by using double extraction in which methanol: water (50:50) was used as an extractant along with process of sonication which was then further detected using UV visible spectroscopy. Seven samples were screened for the presence of sildenafil citrate and out of which two were found to be contaminated with sildenafil citrate. The proposed method is simple, cost effective, environmental friendly and effective to be used in most of the...
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Posted by admin on Aug 31, 2015 in |
The plants and their materials have been utilized for treatment of various diseases in human and animals which are rich source of antioxidants due to presence of phytonutrients such as vitamins (alpha- tocopherol, Ascorbate, carotenoid) or minerals (zinc, selenium) and flavanoid, polyphenols and flavoproteins which exert their action through scavenging free radicals in vivo system. Hence search for new natural antioxidant is required. Aim of the present study was to determine the antioxidant activity of extracts of different plant materials qualitatively and quantitatively, by in vitro tests. Aqueous and ethanolic extracts were prepared from eight different plant material viz., Phyllanthus emblica (fruit), Tectona grandis (leaves), Solanum nigrum (leaves), Momordica charantia (fruit), Ficus bengalensis (leaves), Asparagus racemosus (roots), Swertia chirata (leaves), Curcuma longa (leaves). Phytochemical analysis of these plant extracts were performed for determination of antioxidant capacity by in vitro qualitative and quantitative tests. In vitro qualitative analysis revealed presence of alkaloid, phenol and flavonoids in aqueous and ethanolic extracts of Tectona grandis followed by Solanum nigrum, Phyllanthus emblica. In...
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Posted by admin on Aug 31, 2015 in |
A series of novel 2- substituted benzothiazole dithiomidocarbonate derivatives were synthesized by the reaction of 2-amino benzothiazole with various halides. The test compounds were screened for their antimicrobial activity (antibacterial and antifungal) by disc diffusion method and the results of the microbial activity indicates that the test compounds exhibited varying degree of antimicrobial activity. Among the substituents benzyl moiety exhibited the most potent activity and the compound Dibenzyl (1,3 benzothiazo-2yl) Dithioimidocarbonate(4T1) emerged as the most active antimicrobial agent and it is equipotent when compared to the reference standard Ciprofloxacin. Among the substituents propyl moiety exhibited the most potent activity and the compound Dipropyl (1,3 benzothiazo-2yl) Dithioimidocarbonate (4T3) emerged as the most active antifungal agent and it is equipotent when compared to the reference standard ...
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Posted by admin on Aug 31, 2015 in |
The present investigation was carried to determine UV-VIS, FTIR and GC-MS analysis of Ampelocissus latifolia tuberous root. The ethanol extract was examined under visible and UV light for the proximate analysis. The UV‐VIS profile of Ampelocissus latifolia tuberous root ethanol extract showed the peaks at 409 nm, 424 nm and 479 nm with the absorption of 1.682, 1.704 and 1.479 respectively. The FTIR spectrum was revealed the presence of alcohols, aromatic compound, alkanes, aldehydes, ketones, alkenes, amines, amides, nitro compounds, carboxylic acids, ethers, esters and alkyl halides in ethanol extracts of Ampelocissus latifolia tuberous root. GC-MS analysis of ethanol extract of Ampelocissus latifolia tuberous root was revealed the presence of 14 phytochemical compounds. The presence of various functional groups and phytocompounds in Ampelocissus latifolia tuberous root confirm that it act as a most important source of drugs against various...
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Posted by admin on Aug 31, 2015 in |
Alternanthera brasiliana is a medicinal plant used in the folkloric treatment of malaria, pains, infections and diabetes in South-western states of Nigeria. Recent reports indicate some central activities for the plant. This study evaluated ethanolic leaf extract of the plant for novelty-induced behaviour, anxiety, sedation and convulsion in mice. Dried powdered leaves of the plant were extracted with 70% ethanol, filtered and concentrated in vacuo to obtain ethanolic leaf extract (ELE) of A. brasiliana. The acute toxicity (LD50) of the ELE was determined through oral (p.o.) and intraperitoneal (i.p.) routes. ELE (250, 500 and 1000 mg/kg, p.o.) was evaluated for novelty induced behaviours, anxiolytic, sedative and anticonvulsant activities. The LD50 values obtained for the ELE were ≥5000 mg/kg, p.o. and 3808 mg/kg, i.p. The ELE (500-1000 mg/kg) caused significant (p<0.05) increase in rearing [p<0.05, p< 0.01; F(4, 25)= 37.0] and locomotor [p<0.05, p< 0.01; F(4, 25)= 36.0] activities; caused increase in the number of head dips at 1000 mg/kg [p<0.01, F(4, 25) = 9.0] and non-significantly increased time spent...
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