Posted by admin on Jan 1, 2010 in |
Cellular damage arising from free radical is one of the fundamental mechanism underlying a number of human neurodegenerative disorder like diabetes, inflammation, Alzheimer’s disease, autoimmune pathologic and digestive system disorder. Thus antioxidant plays an important role in the treatment of such disease. The present study aims at a comparative evaluation of ethyl acetate, hexane and methanol extract of Vitex leucoxylon Linn. bark for antioxidant activity. Vitex leucoxylon Linn. a medicinal plant of the verbenaceae family, used in traditional medicine for relieving headache and catarrh. HIME was 1.8964 μg/ml after 48 h of incubation. In this study, it was observed that HIME induces a concentration dependent inhibition of HT29 cells, with an IC50 value of 1.8964 μg/ml after 48 h of...
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Posted by admin on Jan 1, 2010 in |
Natural products represent a reservoir of diverse templates and are being tapped to outsource novel anticancer agents. Hemidesmus indicus R.Br.(Fam: Asclepidaceae) has been reported to be useful for the treatment of inflammation, cuts, wounds, burns, snake bite, skin and blood diseases, ulcers, immunological disorders . In the present study, the methanolic extract of the roots of Hemidesmus indicus, was investigated against human colon cancer cell line (HT29) to explore its anticancer potential. The effect of Hemidesmus indicus methanolic extract(HIME)on proliferation of HT29 cancer cell line was determined by microculture tetrazolium assay (MTT). The cells were exposed to different concentrations (100, 50 , 25, 12.5 , 6.25, 3.125 and1.5 μg/ml) of HIME or vehicle for 48 h. Cisplatin (5, 2.5 and 1.25 μg/ml) acted as positive control and vehicle (DMSO) as negative control. Following treatment, the cells were exposed to Tetrazolium dye (5mg/ml) for 4 h. The formation of the purple coloured formazan complex was dissolved by adding DMSO (100 μl) and read at 490 nm using ELISA microtiter plate...
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Posted by admin on Jan 1, 2010 in |
The practice of traditional medicine for the control of fertility in most parts of India is based on the uses of plant medicines for many years. The fact that herbal medicines have been employed for such a long time does not guarantee their efficacy and safety. The aim of the present study was, therefore, to carry out phytochemical screening, efficacy and safety studies on one of the traditionally used antifertility plants: Curcuma aromatica Salisb. The secondary metabolites of the rhizomes of this plant were determined. The ethanolic and aqueous extract of the rhizomes of this plant were investigated for their antifertility activity in female rats The identification of the secondary metabolites showed that the rhizomes of the plant contained phytosterols, alkaloids, carbohydrate and saponins. The ethanolic and aqueous extract of this plant at two different doses of 200mg kg -1 and 400 mg kg -1 b.w. prevented the pregnancy. The aqueous extract were found to possess more significant (p<0.001) antifertility activity compared to alcoholic extract. All these observations suggest...
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Posted by admin on Jan 1, 2010 in |
Various extracts of Tectona grandis Linn. bark were screened for antiasthmatic activity by using different in-vivo animal models like clonidine induced catalepsy, haloperidol induced catalepsy, milk induced leucocytosis and eosinophilia. The observation of this study indicated that the Tectona grandis bark having antihistaminic activity inhibited clonidine-induced catalepsy and not inhibited haloperidol-induced catalepsy. The results of these studies indicated that ethyl acetate extract of Tectona grandis Linn. Bark showed significant (p< 0.001) antiasthmatic activity at the dose of 100 mg/kg. The anti-asthmatic activity of ethyl acetate extract can be attributed to antihistaminic (H1-antagonist), antimuscarinic, antiallergic, anti-inflammatory and adaptogenic activity suggestive of its potential in management of...
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Posted by admin on Jan 1, 2010 in |
A simple, sensitive and rapid high performance liquid chromatography method with UV detection (215nm) was developed and validated for the quantification of Levetiracetam in human plasma. Following a single-step liquid–liquid extraction with diethyl ether/dichloromethane (70/30 v/v), the analyte and internal standard (Zonisamide) were separated using an isocratic mobile phase, mixture of buffer (5mM Di-Potassium hydrogen phosphate anhydrous, pH7.2): Methanol (85:15 v/v) on reverse phase C-8 Kromasil column. The lower limit of quantization was 1µg/mL, with a relative standard deviation of less than 20%. A linear range of 1µg/mL to 40µg/mL was established. This HPLC method was validated with between and within-batch precision of 5.6–8.9% and 3.9-5.3%, respectively. The between and within batch accuracy was 99.9-106.3% and 96.2-102.0%, respectively. Frequently co-administered drugs did not interfere with the described methodology. Stability of Levetiracetam in plasma was >90%, with no evidence of degradation during sample processing (autosampler) and 60 days storage in a freezer. This validated method is sensitive and simple with between-batch precision of <10% and was used in pharmacokinetic...
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