Posted by admin on Dec 1, 2010 in |
Ethanolic extracts of Rivea hypocraterformis, Methanolic extract of Cynodon dactylone and Water extract of Balanite aegypticae were tested for their in vitro antilithiatic /anticalcification activity by the homogenous precipitation method. The extracts were compared with an aqueous extract of cystone (a marketed preparation) for their activities. Extracts of Rivea hypocraterformis showed activity almost equivalent to cystone while Cynodon dactylone and Balanite aegypticae showed less...
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Posted by admin on Dec 1, 2010 in |
A simple reversed phase HPLC method has been developed for the simultaneous determination of olmesartan medoxomil in combination with hydrochlorothiazide. The method was based on reversed phase liquid chromatography using a Grace Smart RP C18 column (250 × 4.6 mm, 5µ) with UV detection at 256 nm. The mobile phase consisting of acetonitrile and triethylamine buffer adjusted to pH 2.7 in a ratio of (30:70, v/v) and at a flow rate of 2 ml/min. The method was linear over the concentration range for olmesartan medoxomil 4.0-40.0 μg/mL and for hydrochlorothiazide 5.0-50.0 μg/mL. The recoveries of olmesartan medoxomil and hydrochlorothiazide were found to be in the range of 99.25-100.46% and 99.31-102.13% respectively. The method was validated and was successfully employed for the analysis of pharmaceutical formulations containing olmesartan medoxomil and hydrochlorothiazide in combined tablet dosage...
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Posted by admin on Dec 1, 2010 in |
The present investigation was taken up to prepare and evaluate a transdermal drug delivery system (patch) of Raloxifene Hydrochloride to increase its bioavailability. The matrix type patches were prepared using different ratios of Eudragit RL100, Poly vinyl Pyrrolidone K-30, HPMC, CAP and PEG6000 in different ratios. All the prepared formulations were subjected to physical studies (weight variation, thickness, moisture content, moisture uptake, flatness, Water Vapour transmission rate, folding endurance, tensile strength, and % elongation), thumb tack test, in vitro release studies, and in vitro diffusion studies. In vitro permeation studies were performed using artificial membrane and across skin derived from albino rat. The accelerated stability studies for the formulations were performed as per the ICH guidelines. The studies showed that formulation prepared with HPMC and CAP in ratio 5:2 with adhesive polyisobutylene and Eudragit RL100 & PVP in ratio 6:4 (self sticking) were the effective systems to achieve desired results. The interaction studies carried out by comparing the results of TLC, Infrared and UV analysis of pure drug and...
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Posted by admin on Dec 1, 2010 in |
Gene transfer represents a relatively new way for the treatment of rare genetic disorders by changing the expression of a person’s genes. Electroporation is a mechanical method of gene transfer used to introduce polar molecules such as DNA, RNA and Peptides (Heparin, Insulin) into a host cell through the cell membrane. In this procedure, a large electric pulse temporarily disturbs the phospholipid bilayer, allowing molecules like DNA to pass into the cell. With careful control of the strength of the electrical field and the duration of the cell’s exposure to it, the pores will reseal themselves after a short period of time. There are nine ways for gene transfer: 1. Electroporation; 2. Lipid mediated method; 3. Chemical mediated gene transfer; 4. Microinjection; 5. Biolistics; 6. Viral vectors; 7. Transformation; 8. Conjunction; 9. Gene Transfection enhanced by elevated temperature. Among a number of techniques using lipid, viruses or specialty reagents may be cytotoxic, labor intensive and costly while, Electroporation is a simple, inexpensive, safe, non toxic and non viral method....
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Posted by admin on Dec 1, 2010 in |
Transdermal drug delivery system (TDDS) established itself as an integral part of novel drug delivery systems which employ a structure as a reservoir for the drugs. A number of drugs have reached the market in transdermal delivery form that has become an increasingly acceptable mode for administration of prescription and nonprescription drugs. Transdermal drug delivery system possesses many advantages over conventional modes of drug administration. Ketorolac when combined with tromethamine forms a salt (‘ketorolac tromethamine’), which has a greater aqueous solubility than ketorolac. Ketorolac tromethamine is a Nonsteroidal anti-inflammatory drug useful for short-term management of moderate to severe pain. Ketorolac tromethamine has high analgesic and anti-inflammatory potency; administered orally. The focus of this review article is to bring out different aspect related with the administration and its future impacts on human body after administration of ketorolac in transdermal drug delivery...
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