ION EXCHANGERS: A USEFUL TOOL FOR SEPARATION AND SIMULTANEOUS PURIFICATION OF LYSOSOMAL CYSTEINE PROTEINASES, CATHEPSINS B, H AND L.
Lysosomes of mammalian tissues contain a number of cysteine proteinases such as Cathepsins B, H and L, which participate in intracellular protein degradation and are involved in various pathological disorders. These proteinases have been purified to ~ 1600 fold with ~25% yields from goat liver. These Cathepsins having similar molecular weight (~25,000 Da) were obtained as a single pool after initi...
ASPECTS OF VALIDATION IN HPLC METHOD DEVELOPMENT FOR PHARMACEUTICAL ANALYSIS – COMPARISON OF VALIDATION REQUIREMENTS BY FDA, USP AND ICH
The most important factor in developing pharmaceutical drug substances and drug products today is ensuring that the HPLC analytical test methods that are used to analyze the products generate reliable, reproducible and meaningful results. The US Food and Drug Administration (FDA) and United States Pharmacopeia (USP) have each documented the importance of this to the drug development process and ha...
The plant Kalanchoe pinnata is widely used in ayurvedic system of medicine as astringent, analgesic, carminative and also useful in diarrhea and vomiting. Naturalized throughout the hot and moist parts of India. And lots of phytochemical and pharmacological work has done on leaves of plant but the root part is not focused. Hence we have selected roots for phytochemical analysis which will support ...
Colocasia esculenta belonging to family araceae popularly knows as “taro”, is very useful medicinal plant described by Charaka as an anti-inflammatory plant. An experiment was carried out to study the antimicrobial activity of chloroform and methanol extract of Colocasia esculenta plant by agar diffusion method. Zone of inhibition produced by chloroform and methanol extract in dose of 20, 10, ...
Anxiety is a persistent feeling of dread, apprehension and impending disaster, or tension and uneasiness. The reported prevalence rates of psychiatric morbidity in the Indian industrial population range from 14-37%. Anxiety disorders may develop from a complex set of risk factors including genetics, brain chemistry, personality and life events. It is a specific class of psychopathology characteriz...
The conventional chemotherapeutic agents in oncology drug discovery still exhibit poor specificity in reaching tumor site and often restricted by dose-limiting toxicity. The combination of developing drug formulation by utilizing both controlled release technology and drug targeting technology may provide a more efficient and less harmful solution to conquer the limitations found in conventional c...
A number of aldehydes were reacted with acetyl acetone or ethyl acetoacetate and urea under Biginelli reaction conditions to give the corresponding 5-acyl-4-(substituted-aryl)-6-methyl-3,4-dihydropyrimidin-2-one. The 5-acetyl-derivatives were converted into pyrimido-oxazoles, pyrimido-pyrazoles, and chalcones. Some Mannich bases were also synthesized. Some selected compounds were tested for their ...
EVALUATION OF ANTI-INFLAMMATORY ACTIVITY AND TOTAL TANNIN CONTENT FROM THE LEAVES OF BACOPA MONNIERI (LINN.)
The methanolic leaf extract of Bacopa monnieri Linn. (Family: Scrophulariaceae) was evaluated for its anti-inflammatory activity, and total tannins content in different in-vivo and in-vitro methods. The anti-inflammatory activity was studied using carrageenan and histamine-induced rat paw edema test at different doses (200 and 400 mg/kg body weight) of the methanol extract. At the dose of 400 mg/k...
ANTIHYPERGLYCEMIC AND ANTIOXIDANT POTENTIALS OF SESBANIA GRANDIFLORA LEAVES STUDIED IN STZ INDUCED EXPERIMENTAL DIABETIC RATS
Diabetes mellitus is a metabolic syndrome involving severe insulin dysfunction in conjugation with gross abnormalities in glucose homeostasis and lipid metabolism, which has been affecting several millions of population all over the world. Despite the introduction of hypoglycemic agents from natural as well as synthetic sources, diabetes and its secondary complications continue to be a major healt...
Castor (Ricinus communis L, Euphorbiaceous) is needed in the United States to supply caster oil for the hundred of products using this versatile chemurgical raw material. 40-45 thousand tons of castor oil and derivatives are imported each year. Ricinus communis is a common medicinal plant in Ayurveda and is used several part of country for various medicinal properties like the oil of leaf and root...