Posted by admin on Jan 31, 2015 in |
The urease (E.C.3.5.1.5) catalyzes the hydrolysis of urea to ammonia and is commonly used in clinical laboratories for the estimation of urea in biological fluids. The urease enzyme of sword bean (Canavalia gladiata) has been purified in single-step by affinity chromatography using epoxy activated agarose containing urea as the affinity ligand. The yield of the purified enzyme was about 83% with specific activity of about 495 U/mg of protein with 14-fold purification. The final preparation had a transparent appearance with free ammonia content less than 0.01 µg/unit and was stable for 15 months at 4-8°C. The enzyme acted optimally at pH 7.5 and 25°C. Thermal stability studies indicated that at pH 7.5 no loss of enzyme activities were recorded up to 35°C for 30 mins. SDS−polyacrylamide gel electrophoretic analysis showed that the purified enzyme was apparently homogeneous and had a molecular weight of approximately 87 kDa. Single band was observed in both native and...
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Posted by admin on Jan 31, 2015 in |
The purpose of this study was to evaluate antimicrobial activity of Bursera penicillata leaf extract against human infected bacteria. Antimicrobial activity of Petrolium ether, Methanolic and Aqueous extracts from leaves of Bursera penicillata were tested against pathogenic bacteria Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Klebsiella pneumoniae, Staphylococcus saprophyticus, Enterococcus faecalis and Enterobacter cloacae using Agar well diffusion method. Solvent extracts from the leaves of the plant shown a considerable antimicrobial activity against most tested microorganisms. The most active extract was Methanolic extract from the leaf against Klebsiella pneumoniae and significantly inhibited the bacterial activity. Minimum inhibitory concentration for aqueous and methanolic extracts of leaves ranged from 5.0–20.0 mg/ml and ethyl acetate and petroleum ether leaf extracts ranged from 25.0µg-55.0 µg and 45.0-85 µg/ml. It further reflects a hope for the development of many more novel chemotherapeutic agents or templates from such plants which in future may serve for the production of synthetically improved therapeutic...
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Posted by admin on Jan 31, 2015 in |
Solubility is the important parameter to design a dosage form; it is very difficult to formulate an insoluble drug in to final formulation. In This present study deals with the enhancement of solubility of poorly water soluble drug Meloxicam.: The liquisolid system is a novel technique for solubility enhancement and dissolution improvement of low soluble drugs. Liquisolid technique (powdered solution technology) is used to enhance the solubility parameter and dissolution rate of the drug. liquisolids were prepared by using non volatile solvents or hydrophobic carriers (like PEG 400, Tween 80, span 20) and their effect on dissolution of Meloxicam was studied. By using these solvents individually or in combination and find out the optimized formulation with good solubility.Based on the solubility studies the polyethylene glycol 400 (PEG400) is chosen as co solvent, Microcrystalline cellulose (MCC) used as carrier material and Aerosil used as coating material. In liquisolid system different nonvolatile or hydrophobic solvents are continuously added to the liquid until it convert in to a free flowing solid system...
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Posted by admin on Jan 31, 2015 in |
Boric acid is one of the widely used inorganic compound in pharmaceutical industry. We find its utilization as an antiseptic, in eye wash preparations, as an insecticidal, as a buffering agent etc. In this present work, a comparative study of the UV-Vis spectras of the boric acid solution in different solvents have been done. A variety of solvents like water, ethanol, methanol, glacial acetic acid, hexane, Tetrahydrofuran and 0.2M HCl solution have been employed for spectral analysis. When water was taken as a solvent, aqueous boric acid solution gave negative absorbance in complete range of UV-Vis analysis i.e. 1100nm to 190 nm. In contrary to this, when methanolic solution of boric acid was taken, positive absorbance in the complete range of spectrum was noticed. Purely on the basis of spectras obtained for boric acid solution in different solvents, best solvent out of those have been regarded as the most suitable solvent for carrying out the UV-Vis analysis for boric...
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Posted by admin on Jan 31, 2015 in |
Chloroxylon swietenia DC. belongs to the family Rutaceae / Meliaceae / Chloroxylaceae, is a medicinal and aromatic tree of dry deciduous forests. It is popularly known as Yellow wood, East Indian satin wood and Ceylon satin wood. The stem bark is credited for its effectiveness in the treatment of common cough and cold, it is also used as an astringent. Its pharmacognostic data for authentication of the crude drug is not available, hence, in the present study, macroscopical, microscopical, and preliminary phytochemical investigations of stem bark is undertaken. Powder microscopy revealed that Cork cells, Calcium oxalate crystals, Phloem parenchyma, medullary rays and thick walled phloem fibers were abundant. Anatomical studies showed the presence of phellem, phellogen, and phelloderm with abundant secondary phloem. The qualitative chemical tests of petroleum ether, chloroform, acetone, ethanol and water extracts of stem bark revealed the presence of carbohydrates, alkaloids, glycosides, flavonoids, phenolic compounds and...
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