Posted by admin on Nov 1, 2014 in |
The present study evaluates the anti-inflammatory potential of the methanol and chloroform extracts of Cleome viscosa and C. burmanni by in-vitro methods.The anti-inflammatory property of the extracts was assessed using albumin denaturation assay, proteinase inhibitory activityand hyaluronidase inhibition assay and the results compared against reference drugs such as Aspirin and Indomethacin. The present findings exhibited a concentration dependent inhibition of albumin denaturation and hyaluronidase enzyme by the extracts of both plants. Proteinase activity was also significantly inhibited by both plant extracts. The methanol extract was more effective than chloroform extract and was comparable to the standards.From the present study, it can be concluded that the extracts of C. viscosa and C. burmanni possessed marked in- vitro anti-inflammatory activity and the effect could be due to the presence of various phytochemicals present in the...
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Posted by admin on Nov 1, 2014 in |
ABSTRACT: (-)-Hydroxycitric acid (HCA) has been found to be the principal acid in genus Garcinia, which is a potential metabolic regulator of anti-obesity activity and inhibitor of lipogenesis. Presence of (-)-HCA as the principal acid has added importance to thegenus Garcinia. The present study deals with extraction and estimation of (-)-HCA from Garcinia lanceaefolia,collected from four administrative districts of Assam, NE India. An improved HPLC methodology has been developed in the present study for estimation of (-)-HCA. Detection of (-)-HCA was done by observing the orange spot on paper chromatography. The Rf values were observed at 0.34 and 0.45 for free HCA and HCA lactone, respectively. Estimation of (-)-HCA was done by HPLC analysis, using water as mobile phase by taking UV detection at 210 nm and has been found to be 4.56 ± 0.13gm/10gm of dried sample. This is the first report of its kind on Garcinia lanceaefolia...
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Posted by admin on Nov 1, 2014 in |
Two new cardenolide metabolites, 7β-OH-uzarigenin (1M) and 7β-OH-odoroside-H (2M) were obtained through the biotransformation of uzarigenin (1) and odoroside-H (2). Compounds 1 and 2 were isolated from the air-dried roots of Nerium oleander L. The plant, belonging to family Apocynaceae, was collected in October, 2010 from Mansoura university garden in Egypt. The methylene chloride extract of the N. Oleander roots was chromatographed using silica gel column, gradually eluted by petroleum ether: Ethyl acetate with increasing proportions of EtOAc. Further purification by repeated crystallization from hot methanol afforded both compounds. The biological transformation was acheived by Cunninghamella echinulata NRRL 1382 with different incubation periods. The structures of the isolated compounds and the newly assigned metabolites were identified using extensive studies; (1H, 13C, HSQC, HMQC, 1H-1H COSY and 1H-13C HMBC), mass spectral analysis (EI and ESI /MS) and IR....
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Posted by admin on Nov 1, 2014 in |
In the present study, the antidiabetic and antioxidant effect of aqueous extract of Averrhoa bilimbi Linn (ABAE) fruits were evaluated. Diabetes mellitus was induced in male Sprague Dawley rats by a single intraperitoneal injection of streptozotocin (40 mg/kg body weight). Oral administration of aqueous extract of Averrhoa bilimbi Linn (ABAE) (50mg/kg body weight) and metformin (100 mg/kg body weight) to diabetic rats for 60 days significantly reduced the levels of blood glucose, lipids and lipid peroxidation, but increased the activities of plasma insulin and antioxidant enzymes, like catalase, superoxide dismutase, reduced glutathione and glutathione peroxidase. The cytotoxicity of hepatic and renal tissue were ameliorated by treatment with aqueous extract of Averrhoa bilimbi Linn (ABAE) and metformin on histopathological examination. The Averrhoa bilimbi Linn (ABAE) aqueous fruit extract supplementation is useful in controlling the blood glucose level, improves the plasma insulin, lipid metabolism and is beneficial in preventing diabetic complications from lipid peroxidation and antioxidant systems in experimental diabetic rats; therefore, it could be useful for prevention or early treatment...
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Posted by admin on Nov 1, 2014 in |
Plants are the natural reservoir of many anticancer agents. Gardenia latifolia Ait. and Gardenia gummifera Linn. belonging to the family Rubiaceae. Gardenia Ellis. is a genus of 142 species, which occur in tropical and sub-tropical regions of Africa, South Asia, Australasia and oceania. The genus was named by Carl Linnaeus after Dr. Alexander Garden (1730-1791), a Scottish-born American naturalist. Both the plant leaves were extracted by ethanol, extracts were subjected to cytotoxic activity study against MCF-7 cell lines using the MTT assay. MTT assay was the technique utilized for cell survival determination. Measurements were performed and the concentration required for a 50% inhibition of viability (CTC50) was determined graphically. The concentration of ethanolic extract yields the value of CTC50 as 170.00±2.0 µg/ml and 200.00±1.6 µg/ml respectively for Gardenia latifolia Ait. and Gardenia gummifera Linn. The results showed that the Gardenia latifolia Ait. had potential cytotoxicity than the Gardenia gummifera Linn. against MCF-7cell...
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