Posted by admin on Jul 31, 2014 in |
Background &Objective: To explore possible protective effect of neem leaf extract against methotrexate induced genotoxicity in the somatic cells (wing primordial) of Drosophila melanogaster. Methods: In the first phase of the experiment, neem leaf extract (NLE) at two doses was tested in the somatic cells (wing primordia) of Drosophila melanogaster (3rd instar trans heterozygous larvae resulting from cross of two bioactive strains) to probe effect on mutagenicity. In the second phase, effect of NLE at two doses (2.5% & 5%) was studied to probe its role in prevention of methotrexate induced genotoxicity. Concurrent negative control was run simultaneously in each experiment, where the larvae were exposed to the solvent (distilled water). The effect was observed and quantified under microscope as the number of spots (Clones) per wing. Results & interpretation: Only higher concentration of NLE (5%) showed significantly higher spontaneous mutation (2.7%) compared to untreated control group. Methotrexate induced 12.01 spots per wing (positive control). NLE at both doses (2.5 % & 5%) when administered concurrently with methotrexate, average...
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Posted by admin on Jul 31, 2014 in |
In the present work six new benzoxazole derivatives were synthesized adopting the suitably selected scheme. The synthetic methodology included the synthesis of 2-(2-aminophenyl) benzoxazole (D1) by the reaction of anthranilic acid with 2-aminophenol in poly phosphoric acid. Substituted chloroacetanilides (A1-A6) were synthesized by the reaction of substituted anilines with chloroacetyl chloride. Finally compound 2-(2-(benzo[d]oxazol-2-yl) phenylamino)-N-(substituted phenyl) acetamide (2A-2F) were synthesized by the fusion of 2-(2-aminophenyl) benzoxazole (D1) and substituted chloroacetanilides (A1-A6). All the aynthesized compounds were subjected to antimicrobial screening against the bacterial strains i.e., Bacillus subtilis (MTCC-619), Streptococcus pneumonia (recultured), Escherichia coli (NCTC 6571) and Staphylococcus aureus (NCTC 7447). The compounds were also tested for antifungal potential against two fungal strains i.e. Aspergilus. Niger (NCIM NO. 618) and Candida albicans (NCYC 597). In all the testing a significant correlation existed between the antimicrobial potential and the concentration of test...
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Posted by admin on Jul 31, 2014 in |
Objective: Study to elucidate the relation between depression, gender and blood groups. Methods: The study was prepared on 279 medical students of IMTU, who have spent more than four months in the medical school. A Performa for testing depression scale was given to the students. Blood groups and gender were analyzed. Results: It was evident that few blood groups like A and O shown significant prevalence of mild depression and group AB with severe depression. Females shown a high frequency of mild depression and males demonstrated an elevated severe depressed condition. Conclusion: The present study shows that blood groups and gender and depression scales are...
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Posted by admin on Jul 31, 2014 in |
The term ‘transgenic plant’ is defined as the plants that are produced in the lab by artificial insertion of genes sometimes from the same species and sometimes entirely from the different kingdom. A major advantage of transgenic plant because of which it gains a lot of importance in today’s world is developing it for producing pharmaceutically useful compounds. Hence, they are acting as a natural bioreactor for producing therapeutic proteins and peptides in large amount. The present work deals with the investigation of producing a Human Neutrophil Peptide-1 (HNP-1) which is an antimicrobial peptide and commonly known as Defensin in Lycopersicum esculentum Mill. plant by Agrobacterium tumefaciens mediated genetic transformation technique. The cloning of HNP-1 was successfully done in a plant expression vector ‘pGreenI 0029 with 35S CaMV promoter’ by authors and described earlier. In this work we would like to describe transformation of pGreenI 0029 with HNP-1 gene and 35S CaMV promoter, inside Agrobacteium tumefaciens cells and then genetic transformation of Lycopersicum esculentum Mill.plant via recombinant Agrobacteium...
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Posted by admin on Jul 31, 2014 in |
The study is focused on developing a simple, rapid, validated High Performance Liquid Chromatographic (HPLC) method for Glimepiride Tablets from their dosage forms. The detection was carried out at 230 nm using Shimadzu UV – Visible detector HPLC system. The accuracy and precision were determined and validated statistically. The linearity was observed in the range of 15-120 µg/mL with a correlation coefficient of 0.999. The limit of detection and the limit of quantification were found to be 4 ng and 10 ng respectively. A Lichrosorb®(RP-18 column with a mobile phase consisting of acetonitrile – water – glacial acetic acid (550:450:0.6 v/v)) was used. The flow rate was 1 mL/min. The HPLC method is selective, precise and accurate and can be used for routine analysis of preparations in pharmaceutical industry quality control...
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