Posted by admin on Sep 1, 2013 in |
Background: Medicinal plants in Tripathi hills and other developing countries have been scientifically demonstrated to have medicinal benefits but few or none have been translated to products for clinical use. Most herbal products developed by local herbalist and sold to the public are not standardized and lack efficacy and safety data to support use. Objective: to evaluate the medicinal plants for its wound management and test its preclinical safety and efficacy using rat models. Methods: Thirty Wistar albino rats were randomly divided into five groups and wounds were surgically created on the mid dorsal region. The wounds were treated topically and group I served as control group administered with distilled water, group II served as standard, Group III served with MESP, group IV treated with MESI and the group V was treated with METR. The effects of the treatment on the rate of wound closure were assessed. Results: the plant extracts had significantly higher rate of wound closure which was better than distilled water. The significancy was found to...
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Posted by admin on Sep 1, 2013 in |
Nomuraea rileyi (Farlow) Samson is an entomopathogenic fungus capable of producing a variety of enzymes including proteases and chitinases. Protease derived from microorganisms such as fungi, bacteria, and yeast has established wide spread applications in fields such as in the food, detergent and other industries. This study investigates the strategy for partial purification and characterization of protease enzyme from Nomuraea rileyi. The fungus was primarily screened on skim milk agar plate and a clear zone of skim milk hydrolysis confirmed the protease production. The protease activity of 89 U/100 ml was observed in crude sample and then subjected to partial purification by ammonium sulphate precipitation followed by dialysis. The protease enzyme was partially purified to 3.64 fold with a yield of 3.5%. The partially purified protease exhibited optimum activity at pH 8.0 and temperature at 40ºC. Proteases can be used for various commercial purposes such as detergent, waste treatment, leather industry for dehairing the animal skins and degradation of natural proteins. In the present study protease were tested upon...
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Posted by admin on Sep 1, 2013 in |
Quantitative and qualitative improvement of rare and valuable bioactive plant products is promisingly possible using In-vitro cell culture techniques. The present investigation was conducted with the aim to elicit the synthesis ofandrographolide by subjecting cultured cells of Andrographis paniculata to Salicylic acid (SA) and Chitosan mediated abiotic stressCell suspension culture of Andrographis paniculata (Burm. f.) Nees. was treated with salicylic acid and chitosan at late exponential phase. The results were analyzed using High Performance Liquid Chromatography technique. The suspension culture was treated with 0.05mM, 0.5mM and 1.5mM concentrations of salicylic acid for 24, 48 and 72 hrs of treatment duration. The treatment of chitosan was administered to the cell suspension of Andrographis paniculata with the concentrations of 5 mg, 10 mg and 20 mg per 50 ml of suspension culture and each concentration was studied for 24, 48 and 96 hrs of treatment duration. The results revealed that salicylic acid (0.05 mM) for 24 hrs of treatment duration showed as maximum as 18.5 fold increment in andrographolide content (37.0...
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Posted by admin on Sep 1, 2013 in |
Fluvastatin sodium (FVS) is the first fully synthetic 3-hydroxy-3-methylglutaryl coenzyme (HMG-CoA) reductase inhibitors used for the treatment of hypercholesterolemia. It shows extensive first pass metabolism with short plasma half-life (3 hrs). The iontophoresis approach was used to determine the permeability of drug through isolated rat skin. In-vitro permeation of drug was determined by using glickfeld diffusion cell. Cathodal iontophoretic delivery was studied and optimized by evaluating donor compartment for the effect of pH, NaCl concentration, current density, pulsed depolarized DC current and drug concentration. Effectual permeation of FVS was obtained in phosphate buffer pH 5. Different concentration of NaCl showed negative effect on the iontophoretic permeation of drug. At higher current density, the rate of permeation was increased. Pulsed depolarized DC current showed higher flux (34.34 µg/cm2/hr) compared to the continuous DC current (26.44 µg/cm2/hr). With increasing concentration of drug, permeation was increased linearly. Comparison between human cadaver skin and rat skin showed that the permeation of drug was decreased from human cadaver skin (443.311±3.53 µg/cm2) as compare to...
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Posted by admin on Sep 1, 2013 in |
Asthma isa disease of the human respiratory system. The ethanolic extract of the roots of Picrorhiza kurroa was evaluated for antiasthmatic activity by using in-vivo and in-vitro model in guinea pigs. Animal studies used the histamine induced broncho-constriction in guinea pigs. The extract showed a maximum degree of protection of 52.16 % which was comparable to that of salbutamol 65.83 %. The effect of the ethanolic extract of the plant on isolated guinea pig ileum was studies to know the mechanism by which the extract exhibited muscle relaxant activity. The study showed the extract is effective against histamine and acetylcholine induced contraction. The extract exhibited relaxation effect against histamine and acetylcholine induced contraction at a concentration of 100 mg/ml. The result revealed that the ethanolic extract produced antiasthmatic activity due to presence of saponins and...
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