Posted by admin on Jun 1, 2013 in |
Topical corticosteroids have long been the chronic stage in the treatment of steroid responsive dermatitis. Creams containing 0.05% w/w Clobetasol-17-propionate (CP) as active ingredient are categorized as super potent class I topical dermatological corticosteroids. For the drug molecule to reach the cutaneous microcirculation, enhance, the systemic circulation, have to transverse both the lipophilic stratum cornium and much more viable epidermis. Penetration enhancers are thought to intact with one component of skin causing the stratum cornium to swell or leach out some of the structural component their by increasing the drug penetration to the barrier membrane. The current study was carried out to study the effect of various penetration enhancers such as oleic acid (OA), isopropyl myristate (IPM), polysorbate 80 (PS) and thymol (TM) on topical delivery of Clobetasol-17-propionate (CP) and was evaluated in-vitro using cellophane membrane as well as rat skin mounted in Franz diffusion cell. CP was analyzed spectrophotometrically at 240 nm. The efficiency of penetration enhancers to improvise topical delivery of CP was observed in the order...
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Posted by admin on Jun 1, 2013 in |
A simple, specific, precise, and efficient method for the Simultaneous estimation of Metformin HCl and Voglibose tablet by a Reverse Phase-High Performance Liquid Chromatography method is developed and validated. Selected mobile phase were in a combination of acetonitrile: buffer pH- 6.5 in the ratio of 62:38. Optimized column is a stainless steel column packed with base octa decylsilyl silica gel of 250X4.6mm and at 254 nm wavelength for metformin and Voglibose detection by Spectrofluorimeter and excitation wavelength at 350nm and emission wavelength at 430nm. In our study, the validation of analytical method for determination of Metformin and voglibose tablet formulation was performed in accordance the parameters including-system suitability, specificity, linearity of response, accuracy, precision (reproducibility & repeatability), robustness (change of wave length±2 nm). The method is validated according to ICH...
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Posted by admin on Jun 1, 2013 in |
The aim of present study was to develop a new colon targeting formulation, which can minimize the escape of Mesalazine completely in upper gastro-intestinal tract and ensure availability of maximum amount of drug to achieve the desired site i.e. distal colon. The use of press coated tablets with Hydroxypropylmethylcellulose acetate succinate (HPMCAS) and sodium alginate in outer shell was investigated. Two coats (upper and lower) were compressed onto the core tablets of Mesalazine using varying quantities of coating composition i.e. 100mg and 150mg each for lower and upper coat. The Mesalazine tablets coated by compressing 100 mg of HPMCAS each as upper and lower coat did not maintain integrity of the coats and released almost 100% of drug within 3 hrs. The tablets coated by compressing 150 mg of HPMCAS on the core tablets maintained good integrity during the dissolution test and prevented escape of Mesalazine totally in acid stage and buffer sage 1. However, the release of Mesalazine in subsequent buffer stage 2 was also affected. Mesalazine tablets...
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Posted by admin on Jun 1, 2013 in |
Three simple and sensitive and reproducible spectrophotometric methods were developed for the determination of spiramycin (SPM) in pure form and in pharmaceutical formulations (tablets). The first and second methods, A and B, are based on the formation of charge transfer complex between drug and the chromogenic reagents quinalizarin (method A) and alizarin red S (method B) producing charge transfer complexes in methanolic medium which showed an absorption maximum at 568 and 527 nm using methods A and B, respectively. Beer’s law was obeyed in the concentration range of 1.0–10 and 2.0-18 μg mL-1 with mean percentages accuracy of 100.39±0.89 and 100.26±0.60 using methods A and B, respectively. The third method C, is based on the reduction of Fe(III) by spiramycin in acid medium and subsequent interaction of Fe(II) with ferricyanide to form Prussian blue, which exhibits an absorption maximum at 760 nm. Beer’s law was obeyed in the concentration range of 2.0–12 μg mL-1 with mean percentage accuracy of 99.85±0.956. All variables were studied to optimize the reaction conditions...
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Posted by admin on Jun 1, 2013 in |
The extracellular matrix (ECM) is the backbone in controlling cell behavior in the living organisms during the process of wound healing. The design of a suitable biomimetic nanofibrous scaffold which mimics the properties of natural ECM is a need of present time. In order to create a novel and unique wound dressing material, composite nanoscaled eudragit scaffolds loaded with gentamicin were prepared using the process of electrospinning. The nanofibers were characterized for size, size distribution, surface morphology and surface chemical structures using the Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). In-vitro release of gentamicin was also investigated. In vitro drug release tests confirmed that the nanofibers had pH-dependent drug release profiles. These properties indicate that the drug loaded eudragit nanofibrous scaffold has the potential to be used as a candidate in wound healing...
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