Posted by admin on Dec 1, 2012 in |
Due to the limited life span of platelets during regular storage in blood banks, we were interested to investigate the both potential hemostatic effect and dose-dependent response properties of non-viable platelet preparations with injection of two high and low doses in experimental animal model. Infusible platelet membrane (IPM) was prepared from outdated platelet concentrates. Platelet concentrates were pooled, disrupted by freeze-thaw procedure, pasteurized for 20 hours to inactivate possible viral or bacterial contaminants and finally formulated with sucrose and human serum albumin. Rabbits were made thrombocytopenic. Administration of IPM at two doses of 0.5 and 2.0 mg per kg results significant reduction in the bleeding time. The values of bleeding time in the 80 data sets were obtained. Reduction in the percentage of bleeding time elevation during 2, 4, 6 and 24 hours after injections of 0.5 mg/kg were found 56.8, 66.0, 73.7 ,96.8 and after 2.0 mg/kg injections were observed 24.8, 39.0, 52.4 and 95.6 respectively. We concluded that IPM can significantly reduce bleeding time in thrombocytopenic rabbits...
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Posted by admin on Dec 1, 2012 in |
Staphylococcus aureus (S. aureus) is one of the most serious gram-positive bacteria causing several infections. Because of its intrinsic ability to develop resistance to many antibiotics, nowadays only few drugs can be confidently mentioned for the treatment of S. aureus infections. The study aimed to investigate the antibiotics susceptibility pattern of S. aureus among patients. A cross-sectional study was conducted antibiotic susceptibility pattern of S. aureus among patients. A total of 323 wound and nasal swab specimens were collected and inoculated on mannitol salt agar (Oxoid) and incubated at 37oC for 18 to 24 hours. For primary cultures with bacterial growth, Gram-staining and specific biochemical tests (catalase and coagulase) were used to identify the study organism. Sensitivity of the isolates to ten commonly used antibiotics was determined by modified Kirby-Bauer antibiotic sensitivity testing method. The isolates were found to be highly resistant to penicillin G and cephalothin (98.8%; each). Chloramphenicol, amoxicillin/clavulanic acid and oxacillin were also resisted by 53.1%, 50.6% and 38.3% of the isolates, respectively. Least resistance was...
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Posted by admin on Dec 1, 2012 in |
Hyperalgesia is one of the debilitating complications of diabetes. The thermal allodynia and hyperalgesia in diabetic mice may be due to the hyperactivity of C-fiber in the spinal cord. Transient receptor potential vanilloid type 1 (TRPV1) present in spinal cord and activation of C-fibre may involve in hyperalgesia in diabetic mice. Desipramine is one of the tricyclic antidepressants, effective in diabetic neuropathy. The intravenous administration of desipramine depresses the C-fibre reflex that will involve in activation of convergent neurons of the spinal cord. Thus, the present study was carried out to find out the role of TRPV1 in desipramine induced analgesic effect in diabetic hyperalgesia. Mice were administered capsaicin (1 mg kg-1), capsazepine (15 mg kg-1), desipramine (10 mg kg-1) from day 4 to day 11 after induction of diabetes and the nociceptive threshold was measured in terms of reaction time, tail flick latency and tail withdrawal latency. The nociceptive threshold was significantly (p < 0.05) lower in diabetic mice as compared with control group. Capsaicin produced a significant...
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Posted by admin on Dec 1, 2012 in |
A simple, fast and precise reverse phase high performance liquid chromatographic method has been developed for the simultaneous determination of Fexofenadine hydrochloride (FEXO) and Montelukast Sodium (MONT). Efficient chromatographic separation was achieved on phenomenex C18 column (150mm x 4.6 mm, 5 μm) as stationary phase with a mobile phase comprising of 0.5% Orthophosphoric acid pH adjusted to 6 (tri ethyl amine): Acetonitrile(40:60 v/v) at a flow rate of 1.0mL min-1, column temperature of 25°C and UV detection at 240 nm. The retention time of and Fexofenadine hydrochloride and Montelukast Sodium were 2.7 min, and 4 min respectively .The linearity were found to be in the range of 72-120 μg/ml and 6-10 μg/ml Fexofenadine Hydrochloride and Montelukast Sodium respectively with correlation co efficient of 0.999. The proposed method was validated for linearity, accuracy, precision, sensitivity. All validation parameters were within the acceptable range. The developed method was successfully applied to estimate the amount of Fexofenadine hydrochloride and Montelukast Sodium in combined dosage...
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Posted by admin on Dec 1, 2012 in |
In the present study, three algal species (Spirulina platensis, Chlorella pyrenoidosa and Nostoc muscorum (ATCC 2789) were used to investigate their antimicrobial and antioxidant properties against some human pathogenic bacteria and fungi (Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Micrococcus luteus and fungus Aspergillus luchuensis, Aspergillus niger, Fusarium oxysporum). Four different solvents namely methanol, acetone, n-hexane and water were used for extraction. The present investigation showed that methanolic extract was more effective against the pathogenic bacteria and fungi. Methanolic extract of C. pyrenoidosa and N. muscorum was found to be most effective against P. aeruginosa while methanolic extract of S. platensis showed maximum activity against S. aureus. S. platensis showed maximum antifungal activity in comparison to other algal extracts. Antioxidant activity was determined by free radical scavenging activity (Nitric oxide radical scavenging activity) and it was found that methanolic extract of S. platensis showed maximum antioxidant...
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