Posted by admin on Oct 1, 2012 in |
The present work was undertaken to investigate various extracts of fruit of Wrightia tinctoria (F-Apocyanacea) for anti-diabetic activity in alloxan induced diabetic rats. A comparison was made between the action of extracts and known anti-diabetic drug glibenclamide (10mg/kg body weight). Oral administration of methanolic extract at a dose of 300 mg/kg/b. wt and ethyl acetate extract at a dose of 200 mg/kg/b. wt exhibited a significant (p<0.001, p<0.001) hypoglycemic activity in normal rats and significant (p<0.001, p<0.001) anti-hyperglycemic activity in alloxan induced diabetic rats respectively. The maximum reduction in blood glucose level was observed after 4 hours in case of methanolic and ethyl acetate extracts with a percentage protection of 37% and 42% respectively. In long term treatment of alloxan induced diabetic rats the degree of protection was determined by measuring blood glucose on 0, 1, 2, 4, 7, 14th day. Both the extracts showed a significant anti-diabetic activity comparable with that of glibenclamide. These results indicate that the W. tinctoria fruit extracts possess significant anti –diabetic...
Read More
Posted by admin on Oct 1, 2012 in |
The study describes a validated reverse-phase HPLC method for the simultaneous estimation of paracetamol and naproxen in bulk and in tablet formulation. The proposed RP-HPLC method utilizes Eclipse XDB C18 column (150 ×4.6 mm i.d., 5 μm), optimum mobile phase consisted of gradient run of initial ratio of water (pH-2.5 adjusted with orthophosphoric acid): acetonitrile (87:13) with the effluent flow rate of 1.0 ml/min, and UV detection wavelength 263 nm. The developed method was statistically validated for linearity, precision, robustness, ruggedness and specificity. The method was linear over the range of 5-80 μg/ml for paracetamol and 3-48μg/ml for naproxen. The mean recovery was 99.72% and 100.88% for paracetamol and naproxen respectively. The intermediate precision data obtained under different experimental setup was quite concurrent with less critical % RSD. The proposed method can be useful in the quality control of paracetamol and naproxen in bulk drug and drug products....
Read More
Posted by admin on Oct 1, 2012 in |
Excessive platelet activity is one of the most important factors responsible for the incidence of cardiovascular diseases and, also, play important role in coagulation cascade. In this study, the comparative effects of methanol extracts of three herbs on adhesion of the activated platelets to fibrinogen coated plates and clotting factors were investigated. Artemisia dracunculus, Punica granatum and Brberis vulgaris are used as blood anti-coagulatory plants in Iranian folk medicine. Platelets were prepared and incubated with different concentration of the test samples (50-200 μg/ml) for 60 min. The treated and un-treated platelets were then activated with thrombin (0.25U/ml) and their adhesions to fibrinogen coated plates were investigated. Based on obtained data, the methanol extract of Artemisia dracunculus, Brberis vulgaris and Punica granatum at a concentration of 200 μg /ml reduced platelet adhesion to coated wells by 35%, 25% and 20%, respectively. In addition, the effects of the crude extracts of each plant on atherogenic lipoproteins were also examined. The results indicated that the LDL and cholesterol concentration were dramatically reduced...
Read More
Posted by admin on Oct 1, 2012 in |
A simple HPTLC method was used to determine the saponin profile of Barleria cristata L. crude leaf extract. The antimicrobial activity of saponin fraction from the leaves of Barleria cristata L. was studied in-vitro against four bacterial species and four fungal species by agar disc diffusion method. Klebsiella Pneumonia, Staphylococcus aureus, E. coli, Aspergillus parasites were the most inhibited microorganism. The present study suggests that the saponin fraction possess significant antimicrobial activity and can be used to develop a potential antimicrobial...
Read More
Posted by admin on Oct 1, 2012 in |
Condensation reaction of 1, 4-phenylene diamine and Protochatechu aldehyde in 1:2 molar ratio resulted in the formation of new Schiff base ligand 4, 4–(1,4-phenylene bis(azan-1-yl-1-ylidene))bis(methan-1-yl-1-1-ylidene)-1, 2-diol; (H4L) .From the direct reaction of the ligand (1) with Co(II), Ni(II) and Cu(II) chlorides and Fe(III) and Zn(II)nitrates in 2M:1L molar ratio, the five new neutral complexes were prepared. The characterization of the newly formed compounds was done by 1HNMR, U.V-Vis and IR spectroscopy and elemental analysis. The data showed tetrahedral geometry for Co, Ni, Cu and Zn complexes (3, 4, 5 and 6 respectively) and octahedral geometry for Fe complex (2). The in-vitro antibacterial activity against G+ve bacteria (B. subtillus and S. aureus), G -ve bacteria (E. coli and P. aeruginosa) and antifungal activity against (C. albicans and A. niger) of the metal complexes were studied and compared with that of free ligand. Also MIC of the compounds against test microorganisms were...
Read More
