Posted by admin on Jul 1, 2012 in |
Napoleonaea imperialis is used to treat wounds in most parts of Nigeria. Against this background, N-hexane extract of the leaves were screened against some microorganisms to ascertain this claim and to recommend it for further investigation for possible inclusion into official compendium. The plant leaves were dried, powdered and extracted by cold maceration with N-hexane for 24hours. Phytochemical screening was done for alkaloids, saponin, steroidal nucleus, simple sugar, starch, glycoside, proteins and flavonoid using standard procedures. Antimicrobial screenings were carried out using agar diffusion technique. Antibacterial activity was conducted by screening against six pathogens comprising both Gram positive (B. subtilis and S. aureus) and Gram negative bacteria (P. aeruginosa, Klebsiella, E. coli and S. typhi) obtained from pharmaceutical Microbiology laboratory stock. The extract was screened against 24hour broth culture of bacteria seeded in the nutrient agar at concentrations 400, 200, 100, 50, 25, 12.5, 6.25and 3.125 mg/ml in DMSO and incubated at 370C, for 24 hours and measuring the inhibition zone diameter – IZD. The positive controls were ampicillin...
Read More
Posted by admin on Jul 1, 2012 in |
Authorspropose a method to determine the contamination caused during the storage of colchicine stock solution, which will be helpful for the bioanalytical scientists. The process of identification of impurities, exceptionally in this case, started from the point of optimization of the compound, where in the parent ion was not detected and instead an unknown mass is being interpreted by the detector, which was further identified as a plasticizer but not as colchicine. To reconfirm the contamination by the plasticizer, a commercially available colchicine tablet was powdered and extracted with methanol and infused in Q1 MS scan mode. The supernatant solution of Colchicine extract has resulted in a transition of 400.41 m/z which was found to be more predominant. Again the tuning solution of working standard was infused with the same parameters, the same contamination parent peak was found and in addition to this experiment, confirmation against the product ion was also performed, where in the mass transition is found to be 149.177 m/z. Finally, it was found to be...
Read More
Posted by admin on Jul 1, 2012 in |
Argemone mexicana isan indigenous herb commonly known as Prickly poppy. It belongs to the family Papaveraceae. Argemone mexicana is noted to possess medicinal benefits in traditional system of medicine. During last few decades, there has been increasing interest in the study of medicinal properties of this plant and it is reported for Antimicrobial, Antidiabetic, Antioxidant and Hepatoprotective activity. The plant was also reported for other activities like Larvicidal activity, Wound healing activity, Cancer activity, Antihelmintic activity and Neuropharmacological studies. In light of these medicinal properties, this plant can be represented as a valuable source of medicinal compound. This study depicts the overall information about the ethnobotany, phytochemistry and pharmacological activities of the A....
Read More
Posted by admin on Jul 1, 2012 in |
Moxifloxacin Hydrochloride is a fluorinated quinolone antibacterial and Bromfenac Sodium is a non-steroidal anti-inflammatory. The combination formulation is used for the treatment of the reduction of post operative inflammatory conditions of the eye. Three new, simple, accurate and precise UV spectrophotometric methods have been developed and validated for the simultaneous determination of Moxifloxacin Hydrochloride (MOX) and Bromfenac Sodium (BROM) in their combined dosage forms. Method – I is based on simultaneous equation method using two wavelengths, 294 nm (λmax of MOX) and 265 nm (λmax of BROM). Method – II Q‐absorption Ratio method using two wavelengths, 294 nm (λmax of MOX) and 276.66 nm (Isoabsorptive point). Method – III involves the use of First order derivative technique. Here 301.71 nm, the zero crossing point of Bromfenac Sodium, was selected for the determination of Moxifloxacin Hydrochloride and 293.22 nm, the zero crossing point of Moxifloxacin Hydrochloride, was selected for the determination of Bromfenac Sodium. Methanolic HCL (0.1 M) was the solvent used in all three methods. Moxifloxacin showed linearity in...
Read More
Posted by admin on Jul 1, 2012 in |
The antifungal, antimicrobial, antioxidant and cytotoxic activities of methylene chloride, methanol and hydroethanolic extracts of the leaves of Bridelia ferruginea and sterm bark of Pteleopsis suberosa were investigated against six Gram Positive and Gram negative strains bacteria and six species of Aspergilus. The antimicrobial activity was investigatedby the microtest method using p-iodonitrotetrazolium and theantifungal activity by measuringthe mycelial and sporulation inhibition. The phytochemical study was performed on Thin Layer Chromatography and antioxidant activity was assayed using qualitative and quantitative 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging test. The methanolic extract of Pteleopsis suberosa showed better Minimum Inhibitory Concentration against all the tested bacteria. The strongest activity was observed with the methanolic extract of Pteleopsis suberosa against S. aureus meticillin resistant with Minimum Inhibitory Concentration value of 78µg/ml. All extracts showed an interesting sporulation inhibitory percentage up to 60% whereas little to moderate inhibition was obtained against mycelial development (9.18 to 57.53%). The antioxidant activity of extracts increased in dose dependent manner. The methanolic and hydroethanolic extracts were the most active with inhibitory percentage...
Read More
