Posted by admin on Aug 1, 2012 in |
A simple isocratic HPLC method has been developed and validated as per ICH Guidelines for simultaneous and stability indicating determination of Cetirizine Hydrochloride and Ambroxol hydrochloride in pharmaceutical liquid oral dosage form syrup. Separation was achieved in Hypersil BDS C18 column, 5 μ, and 250 mm x 4.60 mm id with flow rate of 1.5 ml/minute and the detection at 230nm. 0.2 molar Ammonium phosphate with the pH of 4.0 with dilute orthophosphoric acid of about 65 percentage in acetonitrile combination was used as a mobile phase. Developed method validated as per ICH Guidelines, prior all the parameter system suitability was carried with five repeated injection of standard solution at the concentration of 50 μg/mL and 30 μg/mL Cetirizine Hydrochloride and Ambroxol Hydrochloride respectively. Percent RSD of five replicate injections of Cetirizine Hydrochloride and Ambroxol Hydrochloride and Tailing factor was found below 2.0% and 2.0 overall analyses respectively. A linear response was observed over the concentration range of 25.06 to 75.18 and 15.13 to 45.38ppm for the assay of...
Read More
Posted by admin on Aug 1, 2012 in |
Objective of the present work is to develop orodispersible tablets of Salbutamol sulphate to improve bioavailability, disintegration time, dissolution efficacy and patient compliance. Orodispersible tablets are the fast growing and highly accepted drug delivery system in now days mainly to improve patient compliance. Orodispersible tablets have number of advantages over conventional dosage forms, because of that Orodispersible tablets have emerged as an alternative to conventional dosage forms. Orodispersible tablets dissolve or disintegrates instantly on the patient tongue or buccal mucosa. Orodispersible tablets of solbutamol sulphate were prepared using superdisintegrants, ac di sol, sod.cmc, alginic acid, chitosan, mcc, as diluents by direct compression method. Twelve formulations were prepared using the superdisintegrants at lower, intermediate & higher concentration. Mannitol is used to enhance the organoleptic properties of tablets. Tablets were evaluated for uniformity of weight, hardness, friability, water absorption ratio, dispersion time, disintegration time and in vitro drug release. All the formulations showed disintegration time less than 33mins and drug release by dissolution (100% at the end of 10...
Read More
Posted by admin on Aug 1, 2012 in |
In the present study, a series of some novel benzothaizole derivatives (BZT 1-10) were screened for their antibacterial activity against S. typhi, S. dysenterae, E. coli, S.aureus and B. subtlis employing Disc Diffusion method and taking Agar as culture media. Streptomycin (25µg/ml) was used as a standard drug. The solvent, DMSO used for the preparation of compounds did not show inhibition against the tested organisms which serves as the negative control. After evaluation of results, most of the derivatives have shown efficient diameter of zone of inhibition as compared to control as well as standard which confirms the promising antibacterial activity of the benzothaizole...
Read More
Posted by admin on Aug 1, 2012 in |
The aim of the present work is to develop simple accurate, precise and cost effective UV-Vis spectrophotometric method for the estimation of pyrimethamine in bulk and pharmaceutical dosage form and determination of percent degradation by using different stress condition. The solvent used was methanol and distilled water (40:60) and λmax of the drug was found to be 272.5nm. A linear response was observed in the range 2-20 µg/ml with a regression coefficient 0.999. The method was then validated for different parameters as per the ICH guidelines. This method can be used for the determination of pyrimethamine in quality control of formulation without interference of the excipients. Pyrimethamine was subjected to stress degradation under different conditions recommended by ICH. The samples generated were used for degradation studies using the developed...
Read More
Posted by admin on Aug 1, 2012 in |
Indigofera linifolia Retz(Fabaceae) is herb which spread throughout the India and found to be used by the tribal to cure inflammations. Based upon the review the present study focused on screening of Ant-inflammatory and Anti-bacterial activity of Indigofera linifolia Retz. The coarse powder of whole plant was extracted by cold maceration method using various solvents successively. Preliminary phytochemical screening was carried out to confirm the presence of phytoconstituents. Based upon the phytochemical tests chloroform and ethanol extracts (200mg/kg) were taken for anti-inflammatory and antibacterial activity screening. Anti inflammatory studies was carried out by Carrageenan induced paw edema method in Wistar albino rats. Anti bacterial screening was carried out in chloroform and ethanolic extract by disc diffusion method in three different concentrations (100μg, 150μg, 200μg /disc) and the diameter of the zone of inhibition were recorded. The percentage increase in paw edema was reduced in both chloroform (18.20±1.32) and ethanol extract (24.22±1.12) treated animals when compared with standard Indomethacin (19.18±1.02) and control (40.10±1.22) animals. The chloroform extract possessed significant anti...
Read More
