Posted by admin on Jul 1, 2012 in |
In this study, Monolayer matrix (MLM) tablet and triple layer matrix (TLM) tablet formulation of metoprolol succinate were fabricated by using Hydroxypropyl-methylcellulose and Polymethacrylates (Eudragit) as the matrix forming agent in both the tablet core layer and barrier layers. The prepared tablets were analyzed for their drug content and in-vitro drug release studies. In-vitro evaluation and comparison of the MLM dosage form and TLM dosage form was done. The role of impermeable barrier layer in controlling the drug release from the core was studied. The in-vitro dissolution studies were carried out and showed a significant difference statistically (P value > 0.05 by ANOVA tool). Mean dissolution time (MDT) increased, while dissolution efficiency (DE %) decreased, indicating that the release of metoprolol succinate is slower from triple layer matrix tablets. The thermal analysis studies (DSC) performed on the initial TLM formulation and three month old accelerated stability sample of the same showed no variation in the thermograph, indicating TLM as stable formulation. The finding of the study indicated that the...
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Posted by admin on Jul 1, 2012 in |
Multivitamin tablets containing various substances of varying characteristics may have a problem in quantitative analysis. This research has developed HPLC method for simultaneous determination of three vitamin components that is thiamine (Vit. B1), riboflavin (Vit. B2) and pyridoxine (Vit. B3) in tablet formulation. The chromatographic separation was achieved by using a C-18 column with dimension of 4.6 mm I.D.X 250 mm and particle size of 5μm. A mixture of methanol: water (22:78) was used as mobile phase. The aqueous mobile phase contained O- phosphoric acid adjusted to pH 2.5, with flow rate of 1mL/min. The effluent was monitored at 290 nm at ambient temperature. Effective separation and quantification was achieved in less than 10 min. The method was simple, accurate, precise, and could be successfully applied for the analysis of thiamine, riboflavin, and pyridoxine multivitamin tablets. The HPLC results revealed that % vitamins were found to be for B1, B2 and B6 respectively in marketed multivitamin formulation. HPLC analysis of biological fluid (urine) showed that % Vit.B1 in 2,...
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Posted by admin on Jul 1, 2012 in |
A simple, accurate, precise, economical analytical method has been developed for the simultaneous estimation of abacavir and lamivudine in pure bulk drug and in combined tablet dosage form by UV spectrophotometry in multicomponent mode. The standard solutions of abacavir and lamivudine in mixture of acetonitrile and methanol were diluted with distilled water individually to get the concentration of (20 µg/mL and 10 µg/mL) and the scanning range between 200 nm to 350 nm was selected. The sampling wavelengths 224, 241, 257, 280 and 296 nm was selected and the concentrations of individual drugs in five mixed standard solutions were fed to the multicomponent mode of the instrument. The results of analysis have been validated as per ICH guidelines and were found to be satisfactory. Hence, present study gives excellent methods for the determination of both the drugs in combined tablet formulation with relative...
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Posted by admin on Jul 1, 2012 in |
Napoleonaea imperialis is used to treat wounds in most parts of Nigeria. Against this background, N-hexane extract of the leaves were screened against some microorganisms to ascertain this claim and to recommend it for further investigation for possible inclusion into official compendium. The plant leaves were dried, powdered and extracted by cold maceration with N-hexane for 24hours. Phytochemical screening was done for alkaloids, saponin, steroidal nucleus, simple sugar, starch, glycoside, proteins and flavonoid using standard procedures. Antimicrobial screenings were carried out using agar diffusion technique. Antibacterial activity was conducted by screening against six pathogens comprising both Gram positive (B. subtilis and S. aureus) and Gram negative bacteria (P. aeruginosa, Klebsiella, E. coli and S. typhi) obtained from pharmaceutical Microbiology laboratory stock. The extract was screened against 24hour broth culture of bacteria seeded in the nutrient agar at concentrations 400, 200, 100, 50, 25, 12.5, 6.25and 3.125 mg/ml in DMSO and incubated at 370C, for 24 hours and measuring the inhibition zone diameter – IZD. The positive controls were ampicillin...
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Posted by admin on Jul 1, 2012 in |
Authorspropose a method to determine the contamination caused during the storage of colchicine stock solution, which will be helpful for the bioanalytical scientists. The process of identification of impurities, exceptionally in this case, started from the point of optimization of the compound, where in the parent ion was not detected and instead an unknown mass is being interpreted by the detector, which was further identified as a plasticizer but not as colchicine. To reconfirm the contamination by the plasticizer, a commercially available colchicine tablet was powdered and extracted with methanol and infused in Q1 MS scan mode. The supernatant solution of Colchicine extract has resulted in a transition of 400.41 m/z which was found to be more predominant. Again the tuning solution of working standard was infused with the same parameters, the same contamination parent peak was found and in addition to this experiment, confirmation against the product ion was also performed, where in the mass transition is found to be 149.177 m/z. Finally, it was found to be...
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