Posted by admin on Jul 1, 2012 in |
Schiff base ligand (E)-4-[(hydroxyl phenylimino)methyl]benzene-1, 2-diol (H3L) was prepared from the condensation reaction of Protochatechu aldehyde (3, 4-dihydroxybenzaldhyde) with 2-amino phenol. From the direct reaction of the ligand (H3L) with Co(II), Ni(II) and Cu(II) chlorides and Fe(III) and Zn(II) nitrates in 2M:1L molar ratio, the five new neutral complexes were prepared. The characterization of the newly formed compounds was done by 1HNMR, UV-Vis and IR spectroscopy and elemental analysis. The in-vitro antibacterial activity and antifungal activity of the metal complexes were studied and compared with that of free ligand. The Cupper complex shows the higher biological...
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Posted by admin on Jul 1, 2012 in |
Mitragyna speciosa Korth. is a member of the Rubiaceae family. More than 25 alkaloids have been isolated from Mitragyna speciosa. Mitragynine is the major alkaloid of this plant and is responsible for antinociceptive action. No single study is available about the effect of mitragynine on aminopyrine N-demethylase activity in rat hepatocytes. Experiments were undertaken to evaluate the effect of mitragynine in different age groups (adult & old) of Sprague- Dawley (SD) male and female rat hepatocytes. In vitro this evaluation was assessed by different concentration of mitragynine (0.0025µM-250µM).Hepatocytes were prepared by collagenase perfusion technique. Aminopyrine N-demethylase activity was determined by measuring the quantity of formaldehyde formed. Results showed that a significant increase in aminopyrine N-demethylase activity was observed in the adult male, female and old female SD rat hepatocytes treated with 250µM mitragynine (p< 0.05). However, the old male rat did not show any significant change at any concentration of mitragynine. In conclusion this study indicates the induction of hepatic drug metabolizing enzymes by mitragynine is affected by the...
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Posted by admin on Jul 1, 2012 in |
To evaluate the in vivo antioxidant activity of alcoholic extract of the roots of Rubia cordifolia (RC). Male mice, were exposed, either to a diet containing 40mg/kg body weight of Pb nitrate or combined with Rubia cordifolia for the first period of 40 days, where the animals were sacrificed for oxidative studies. Ingestion of Pb (NO3)2 was significantly decreased RBC count, WBC count, Hb level and serum total protein contents in the mice. But, serum Alanine Transaminase, Aspartate Aminotransferase, and cholesterol level were significantly increased after implication of this metal. However, oral administration of Rubia cordifolia, to lead treated mice led to marked improvement in both hematological and serum biochemical changes. Rubia cordifolia alone had moderate effect on mice, where as co-administration of lead nitrate with Rubia cordifolia reversed the effect of...
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Posted by admin on Jul 1, 2012 in |
Arjunarishta, also known as Parthadhyarishta, is a polyherbal hydro alcoholic formulation and is advised as a choice of remedy in cardiovascular disorders. A simple, precise and accurate HPTLC method has been established for the determination of quercetin and rutin in Arjunarishta–T and Arjunarishta-M prepared by traditional and modern methods respectively and also in its marketed formulation. The developed HPTLC method was validated in terms of precision, accuracy, LOD, LOQ, specificity, robustness and ruggedness. The amount of gallic acid in Arjunarishta-T, M and its marketed formulation was found to be 0.0332, 0.0331 and 0.0330% w/w respectively while ellagic acid was found to be 0.0361, 0.0360 and 0.0359% w/w respectively. This is the first report for the quantification of gallic acid and ellagic acid in Arjunarishta by HPTLC. Furthermore, no TLC densitometric methods have been reported for the quantification of gallic acid and ellagic acid from...
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Posted by admin on Jul 1, 2012 in |
Metal ions such as iron , lead, copper, nickel, cadmium , chromium and zinc were investigated in medicinally important plants Alstonia scholaris, Tabernaemontana coronariae, Asparagus racemosus, Mimosa pudica, Leucas aspera and Adhatoda vasica applying atomic absorption spectrophotometer techniques. The purpose of this study was to standardize various metal ion Contamination in indigenous medicinal plants. Maximum concentration of lead was present in Leucas aspera and Adhatoda vasica followed by Alstonia scholaris, Tabernaemontana coronariae and Asparagus racemosus. The concentration of lead in Mimosa pudica was below the detectable level. The maximum concentration of zinc was detected in Adhatoda vasica followed by Leucas aspera, Asparagus racemosus, Tabernaemontana coronariae, Alstonia scholaris and Mimosa pudica. The concentration of Cadmium, nickel and chromium was below the detectable...
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