Posted by admin on Apr 1, 2012 in |
A simple, precise and stability-indicating HPLC method was developed and validated for the simultaneous determination of anti-hypertensive drugs Irbesartan, Losartan, diuretics Hydrochlorothiazide and Chlorthalidone. The separation was achieved on Hypersil BDS (Length 250 mm × Diameter 4.6 mm Particle size 5 μm) column with gradient flow. The mobile phase at a flow rate of 1.0 mL min−1 consisted of 0.05 M sodium dihydrogen phosphate buffer and acetonitrile (Gradient ratio). The UV detection was carried out at 220 nm. The method was successfully validated in accordance to ICH guidelines. Further, the validated method was applied for commercially available pharmaceutical dosage...
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Posted by admin on Apr 1, 2012 in |
The association between TSH and serum lipids in people with no apparent thyroid dysfunction is less understood. We have investigated the association between normal thyroid function, defined as TSH within the reference range and serum lipids concentrations. Cross-sectional, population based study with 1785 individuals without known thyroid dysfunction. We calculated mean concentrations of total serum cholesterol, low-density lipoprotein (LDL) cholesterol, non-high-density lipoprotein (HDL) cholesterol, HDL cholesterol and triglycerides across categories of TSH, using general linear models. Within the reference range of TSH, there was a linear and significant (P for trend <0.001) increase in total serum cholesterol, LDL cholesterol, non-HDL cholesterol and triglycerides, and linear decrease (P for trend <0.001) in HDL cholesterol with increasing TSH. Within the range of TSH that is considered clinically normal, we observed that increasing level of TSH was associated with less favorable lipid concentrations. The association with serum lipids was linear across the entire reference range of...
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Posted by admin on Apr 1, 2012 in |
Lipospheres of benzyl penicillin were formulated using the conventional thin film hydration technique. Five different combinations of shea butter, surfactant (Span 80) and goat fat were the key variables employed in the formulations. The resultant lipospheres were evaluated with respect to surface morphology, particle size distribution, encapsulation efficiency, in vitro drug release, in vivo bioavailability and in vitro antimicrobial activity. Particle size was found to increase with increased drug loading, the average particle radius of the batches being 12.5 nm. The encapsulation efficiency was found to be high at all levels with encouraging values of 80.21 and 83.44 % for batch A and E respectively, with batch A containing shea butter and span 80 in the ratio of 1:1 and batch E containing shea butter, span 80 and goat fat in the ratio of 1:2:1. Batch A appear to exhibit sustained release in vitro with cumulative drug release being 60 % while batch E has a cumulative drug release of 98 % respectively. The presence of goat fat however...
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Posted by admin on Apr 1, 2012 in |
The present study is concerned to investigate and compare physico-chemical properties( thickness, hardness, weight variation, friability and disintegration), invitro dissolution of the drug along with drug content(assay) for different brands of tablets containing pantoprazole (40mg, 20mg) prepared by various pharmaceutical industries under different trade names. All the brands passed all the official tests as prescribed by the Pharmacopoeia. All the brands were within the limit when tested for Thickness, Weight variation, Hardness, Friability and Disintegration. The amount of drug obtained and percentage purity is less when compared to that of labeled claim. Among the four brands, both 40 mg(B) and 20 mg(b), brand 1 i.e. B1 and b1 was found to have more amount of the drug and brand 2 i.e. B2 and b2 was found to have less amount of the drug. The U.V. Spectrophotometric method for the assay of Pantoprazole tablets used in this study is simple, inexpensive, reproducible and easy to use and could be used in routine monitoring of the quality of the pantoprazole sodium...
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Posted by admin on Apr 1, 2012 in |
Antimicrobial and cytotoxic activity of petroleum ether, ethyl acetate and methanol extracts from Juniperus indica Bertolwere evaluated. The extracts screened for their antibacterial activity against human pathogenic bacterial strains, Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae. All the fractions presented antimicrobial activity with variable strengths to all of the tested microorganisms. Phytochemical screening revealed the presence of terpenoids, flavanoids and tannins as main phytochemical groups. Cytotoxic activity was evaluated by using brime shrimp lethality test. LC50 value for the methanolic, ethyl acetate, and petroleum ether extracts were calculated at 550 µg/ml, 380 µg/ml and 140 µg/ml...
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