Posted by admin on Mar 1, 2012 in |
The present experiment was aimed to examine the effect of furosemide (loop diuretic) on ingestive behavior & some hematological parameters. The adult Wistar rats (n=6/ group) were divided into control group (n =6; Group I) and experimental group (n =6; Group II). The experimental group received furosemide intraperitonially (40mg/kg BW) for a duration of 30 days. The control group of rats was injected with distilled water of same volume intraperitonially. Body weight, food and water intake was measured each day. At the end of the experimental procedure; blood samples were collected to measure the hematological parameters such as total leukocyte count (TLC), blood cell (RBC) count , Packed cell volume (PCV) and hemoglobin (Hb) concentration. The results were analyzed statistically by using student‘t test. P < 0.05 was considered as significant. Our present results showed that, administration of furosemide showed a significant increase (P < 0.001) in the ingestive behavior and body weight. Further, the RBC count, PCV, Hb concentration also showed a significant increase (P < 0.05) whereas...
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Posted by admin on Mar 1, 2012 in |
In the present study, 3-D QSAR analysis was performed on the previously synthesized and evaluated derivatives of novel 2-arylthiazolidinones as selective analgesic N-type calcium channel blockers. Calcium Channel blockers is the molecular target responsible for the treatment of neuropathic and inflammatory pain. The 3D-QSAR study based on the principle of the alignment of pharmacophoric features by PHASE module of Schrodinger suite has been carried out on the same set of calcium channel blockers. Statistically significant 3-D QSAR model (R2=0.9288) were generated using 21 molecules in the training set. The predictive ability of model was determined using a randomly chosen test set of 6 molecules which gave predictive correlation coefficients (R2pred) of 0.946 for 3-D models, indicating good predictive power. PHASE pharmacophore hypothesis AAHR.13 may correspond very closely to the interactions recorded in the active site of the ligand bound complex. These studies produced models with high correlation coefficient and good predictive abilities. Docking studies were also carried out wherein these analogues were docked into the active sites of COX-2...
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Posted by admin on Feb 1, 2012 in |
Gastroretentive delivery systems can be retained in the stomach and assist in improving absorption and consequently the bioavailability of drug that has a narrow absorption window in a particular region of gastrointestinal tract. A floatable multiparticulate system with potential for intragastric sustained delivery is one of the approaches to get the gastroretention. Cinnarizine(CNZ), an antihistaminic drug used in vertigo caused by meniere’s disease was taken as a model drug for floating beads prepared by non effervescent method. Floating CNZ olive oil-entrapped emulsion gel beads were prepared by the emulsion–gelation method. Different concentrations of sodium alginate (1%, 2%, and 3% w/v), oil (5%, 10%, and 15% v/v), and calcium chloride (0.02, 0.1, and 0.5M) were used and their influence on beads uniformity, buoyancy, and in vitro drug release was studied. The results indicated that retardation of drug release was achieved by the oil hydrophobic diffusion barrier, especially in the presence of the compact network of alginate beads. The selected formula of calcium alginate beads using 3% w/v sodium alginate, 15%...
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Posted by admin on Feb 1, 2012 in |
Crude ethanol and methanol extracts from dried and fresh leaves of Simarouba glauca were tested for their inhibitory activity against two food borne pathogenic microorganisms (Staphylococcus aeureus and Escherichia coli) and two food spoilage microorganism (Bacillus subtilis and Pseudomonas aeurogenosa). Screening for antimicrobial activity using well diffusion assay showed the inhibition against entire tested microorganisms. On the other hand the maximum zone of inhibition was recorded of fresh leaves methanol extract (FLM) about 11 mm against Escherichia coli and the lowest zone of inhibition was recorded of fresh leaves methanol extract (FLM) about 2 mm against Bacillus subtilis. Minimum inhibitory concentrations (MIC’s) of extracts were determined using agar dilution method on the same test microorganisms. Fresh leaves methanol (FLM) extract gave MIC value ranging from 160 to 10,240 parts per million (ppm). Result showed that the Bacillus subtilis was the most sensitive...
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Posted by admin on Feb 1, 2012 in |
Riboflavin binding protein (RfBP) was isolated from the eggs of Aquila hastate (eagle). The protein was purified in two steps, DEAE – Sepharose ion exchange chromatography and gelfilteration on Sephadex G-100. The holoprotein had an absorption spectrum characteristic of flavoproteins. The purity of the protein was judged by SDS-PAGE technique. A single band on the slab and cylindrical gel revealed that the protein was pure comparison of the mobility of RfBP with that of the standard molecular weight marker proteins suggested that RfBP from the egg white of Aquila hastate had a molecular weight close to 29...
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