Posted by admin on May 1, 2012 in |
The antioxidant activity of methanol extract of whole plant of Synedrella nodiflora as well as its petroleum ether, carbon tetrachloride, dichloro-methane and aqueous soluble partitionates were evaluated by DPPH of (1, 1-diphenyl-2-picrylhydrazyl) and phosphomolybdenum total antioxidant assay and compared with standard antioxidants butylated hydroxytoluene (BHT) and ascorbic acid (ASA). The total phenolic content was also determined and expressed in gallic acid equivalent (mg of GAE/g of sample). A great variance was observed for polyphenol content as well as antioxidant activity (1.574-9.4136 mg GAE/g and DPPH IC50 10.52-31.25 μg/ml) depending on the nature of solvent used to fractionate the crude extract. The result demonstrated that dichloromethane soluble fraction revealed the highest amount of phenolic compounds (9.4136 mg GAE/g) and also had significant free radical scavenging activity (IC50 10.52 μg/ml). A positive correlation was observed between total phenolic content and total antioxidant activity of S. nodiflora having correlation coefficient (R2) of 0.9270. The general toxicity of the extractive was studied by brine shrimp lethality bioassay and from the results (LC50 0.023-0.122...
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Posted by admin on May 1, 2012 in |
In Ethiopian traditional medicine, the aqueous decoction of the leaves of Cineraria abyssinica Sch. Bip. exA. Rich (Asteraceae) is used for treatments of various ailments including diarrhea, however, to date, there appear to have been no reports on the phytochemistry and the antimicrobial activity of the plant. The main aim of this study was, therefore, to carry out preliminary phytochemical screening and antimicrobial activities on leaf extracts of C. abyssinica. The in vitro antimicrobial activities of the aqueous and 80% methanolic crude extracts of the leaves of C. abyssinica were investigated against Gram positive and Gram negative bacteria by agar disc diffusion method. Both the aqueous and 80% methanolic extracts showed various degrees of potent antibacterial activities comparable to the standard drug ciprofloxacin against all of the bacteria tested except Bacillus species. Preliminary phytochemical screening of the plant revealed the presence of polyphenols, flavonoids, coumarins, saponins, terpenoids and phytosterols. The acute toxicity studies showed the nontoxic nature of the plant up to 3 g/kg. Therefore, the present study revealed...
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Posted by admin on May 1, 2012 in |
The curative parts of medicinal plants are not simply its woody stem or its leaves but the number of chemical compounds (phytoconstituents) it produces and uses for its own growth like flavonoids, alkaloids, gum, mucilage, protein, carbohydrate etc. The genus Phyllanthus is widely used in medicine but still lot of work left to explore this genus. So the species, Phyllanthus emblica which is widely used in Indian traditional system are selected for various screening. The mentioned plant materials are rich source of polyphenolic compounds and some typical type of polyphenolic are responsible for various pharmacological activities. The isolation and characterization of Phytoconstituents was done from the alcoholic extract by the chromatographic method. The structure of the isolated compound was established on the basis of physical, chemical test and spectroscopic evidences (IR, UV, 1HNMR, MS). A flavonoidal structure was isolated from the alcoholic extract of the plant. The yellow colored compound which solubilizes in methanol, ethanol and water having m.p. 315oC and Rf value 0.19 in mobile phase Isopropanol: formic...
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Posted by admin on May 1, 2012 in |
Two chemical compounds have been isolated and their structures were elucidated by spectroscopic studies including VLC, TLC and NMR. The isolated compounds are Ortho-hydroxy-N- benzyl- 16- Methyl- 11, 14- diene-octadecamide (1) and 9, 12-diene–octadecanoic acid...
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Posted by admin on May 1, 2012 in |
Mycotic infections of skin are caused by dermatophytes. Screening of plants for anti dermatophyte activity was carried out based on the literature search done. Native plants of Maharashtra (India) were screened for anti dermatophyte activity. Various plant parts from different regions were collected and then extracted with three different solvents viz. alcohol, hydro-alcohol and aqueous. The obtained extracts were subjected for anti dermatophyte activity using agar-well diffusion technique. Three different concentrations of extract were checked for activity. Two species of dermatophytes, viz. – Trichophyton and Microsporum were used in the screening assay. Out of the twenty-eight plants screened by agar diffusion method, seven were found to be active with different activity profile. Methanol extract was the most active extract. Pterospermum suberifolium, Trachyspermum ammi, Peltaphorum pterocarpum, Ixora coccinia, Persicaria glabra, Terminallia elliptica and Cicca acida showed activity at different concentrations against the two species of dermatophytes. The data obtained can be used for further studying the anti dermatophyte potential of active...
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