Posted by admin on Sep 1, 2011 in |
Purpose: The aim of this study was to enhance the dissolution rate of Cyclosporine A, a poorly water-soluble drug by developing a self micro-emulsifying (SME) tablet formulation by using the liquisolid compact technique. A liquisolid system is formed by converting a liquid formulation into a dry, free-flowing and compressible powder mixture with selected carrier material and coating material. This technique has industrial applications for low dose insoluble drugs. Method and Results: The solubility of Cyclosporine A (BCS ClassⅡdrug), as a model drug in this study, was determined in several oils, surfactants and co-surfactants using an HPLC method. The self micro-emulsifying system of Cyclosporine A was constructed by using Maisine 35-1 and Lauroglycol FCC (1:1, w/w, oil phase), PEG-35 Castor Oil (surfactant) and PEG 400 (co-surfactant). The ratio of these components in the formulation was 20:50:30 (w/w) and optimized by a pseudo ternary phase diagram. The droplet size of the optimized liquid with drug was 32.9±0.1nm. The stability experiment results showed the model drug in the micro-emulsifying system was stable...
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Posted by admin on Sep 1, 2011 in |
Analytical methods employed for the determination of drugs and metabolites in biological matrices such as urine, plasma and serum are essential throughout drug discovery and development. It is well-known that, analytical techniques are constantly undergoing change and improvements and each analytical method has its own characteristics which may vary from analyte to analyte at different conditions. As the drug continues through development, the decisions become more critical; therefore, the bio-analytical methods that produce the data should be accurate. In the present study, we have developed a simple, precise and reproducible liquid chromatography tandem mass spectrometry method and validated according to FDA-GLP guidelines for quantification of Omeprazole in human plasma using Lansoprazole as internal standard utilizing LC-MS/MS incorporated with quadrapole mass spectrometer utilizing electrospray ionization technique. Samples were analyzed for Accuracy, Precision, Sensitivity, Selectivity, Recovery, stability and accuracy of method by injecting spiked matrix (human plasma) in to LC-MS/MS. The method specificity was determined by analyzing six different batches of human plasma to check the chromatographic conditions from endogenous plasma...
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Posted by admin on Sep 1, 2011 in |
Ethosomal systems are now a days attracting attention of many researchers. This study is designed to observe the effect of penetration enhancers in ethosomal formulations. Ascorbic acid is taken as a model drug; phosphatidylcholine and ethanol are taken for ethosome preparation. Propylene glycol is taken as a penetration enhancer. Ethosomes are prepared by solvent dispersion method with and without penetration enhancer. The drug release profile is compared. Dialysis membrane and human cadaver skin are taken for penetration study. In this study the ethosomes which were prepared by adding penetration enhancers showed better penetration...
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Posted by admin on Sep 1, 2011 in |
A simple, reproducible and efficient spectroscopic method developed and validated for determination of Orlistat in bulk and capsule dosage form. The drug was determined spectrophotometrically at 203 nm using methanol as a solvent. The percentage recovery study of the drug for the proposed method ranges from 100.17-99.53%w/w indicating no interferences of the capsule excipients. Linearity was obtained in the concentration range 10-100μg/ml with correlation coefficient of 0.9982. The result analysis was validated statistically and recovery studies confirmed the accuracy and precision of the proposed...
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Posted by admin on Sep 1, 2011 in |
Mucoadhesive microcapsules of sodium valproate, an anticonvulsant used in the treatment of epilepsy and in migraine, have been prepared from sodium alginate, hydroxypropylmethyl cellulose-K4M, carbopol 934P & sodium CMC using 10% w/v calcium chloride solution by ionic gelation method. Drug: polymer ratios were 1:1 in all the formulations and polymer mixtures employed were 4:1, 5:1, 6:1, 7:1 of sodium alginate: polymer (hydroxypropyl methyl cellulose-K4M, carbopol 934P & sodium CMC). Calcium chloride was used for ionic gelatin and cross linking of sodium alginate molecules. Microcapsules were spherical in shape and of sizes between 798 microns to 952 microns. Carbopol 934P was found most effective in controlling drug release from microcapsules followed by sodium CMC. Drug release found to be best from the formulation developed with carbopol 934P and followed Super case II...
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