Posted by admin on Dec 1, 2011 in |
Tinospora cordifolia (Fam: Menispermaceae) has been investigated for evaluation of the biological activities. The stem of Tinospora cordifolia were extracted with carbon tetrachloride, n-hexane and methanol were collected and afford 4.0 mg, 4.0 mg, and 4.0 mg, respectively for the test. The crude carbon tetrachloride, n-hexane, and methanol extract of the plant were subjected to antimicrobial screening against 15 microorganisms such as gram-positive, gram-negative, fungi by the disc diffusion method. But interestingly no fraction showed any inhibitory effect against all the microorganisms. In the brine shrimp lethality bioassay, carbon tetrachloride fraction showed high toxicity, where LC90 value was only 6.25 mg/ml. The methanol crude extract and n-hexane fraction showed LC50 of 1.563 and 6.05 mg/ml respectively with very narrow therapeutic index (LC90 of 43.0 mg/ml and 45.0 mg/ml respectively. This indicated that the cytotoxicity exhibited by the carbon tetra chloride, n-hexane, and methanol extract was very...
Read More
Posted by admin on Dec 1, 2011 in |
Artemisia pallens Walls. ex DC, commonly known as Davana, is an aromatic herb found abundantly in humid habitats in the plains all over India. Artemisia pallens is found in Nilgiri hills, and has been used by the tribal people for various ailments. It has been widely used in Indian folk medicine for the treatment of diabetes mellitus. This plant is accredited with antihelmintic, antipyretic and tonic properties and also considered as a good fodder. The oil possesses antispasmodic, antibacterial, antifungal and stimulant properties. The plant has been screened for the antimicrobial, antidiabetic, antinociceptive and wound healing activity. The current study deals with the Phytochemical and Pharmacological of Artemisia...
Read More
Posted by admin on Nov 1, 2011 in |
Spectrophotometric method has been developed for the determination of Tolterodine tartrate (TOL) which is based on formation of a binary complex between TOL and eosin at 545 nm using acetate buffer at pH 3.8.The absorbance-concentration plot is rectilinear over the range (1-10μg/ml) with LOD of 0.1μg/ml and LOQ of 0.31μg/ml. The proposed method was successfully applied to the analysis of commercial tablets containing the drug and the results were in good agreement with those obtained with the reference method. The method was also utilized to investigate the kinetics of alkaline, acid and oxidative degradation of the drug .It was found to be stable upon acidic and alkaline degradation and only undergo oxidative degradation using 33.3%H2O2. The apparent first order rate constant and t1/2 of the oxidative degradation reaction were...
Read More
Posted by admin on Nov 1, 2011 in |
Urinary tract infection (UTI) remains the common infections diagnosed in outpatients as well as in hospitalized patients. Worldwide data show that there is an increasing resistance among urinary tract pathogens to conventional drugs. Extended spectrum beta lactamases (ESBL) hydrolyse expanded spectrum cephalosporins like ceftazidime, cephotaxime which are used in the treatment of UTI. ESBL-producers are not easily detected by the routine disk diffusion susceptibility test, and this result in the failure of treatments due to inappropriate use of antibiotics. No information on ESBL producing organisms causing UTI is available from Tiruchirappalli, Tamil Nadu. Urinary isolates from symptomatic UTI cases attending or admitted to a hospital in Tiruchirappalli were identified by conventional methods. Antimicrobial susceptibility testing was done by Kirby Bauer’s disc diffusion method. Isolates resistant to cephotaxime were tested for ESBL production by double disc synergy test method. Of the 936 isolates, 236 (25.2%) were found to be ESBL producers. In the present study, a large number of uropathogens were found to be ESBL producers. Most of the ESBL...
Read More
Posted by admin on Nov 1, 2011 in |
The objective of the paper is to study the effect of a permeation enhancer, sodium taurocholate on permeation of Ondansetron HCl from bioadhesive buccal tablet formulation by performing ex-vivo permeation experiments using porcine buccal mucosa. Optimized formulation has selected based on in-vitro drug release studies of bilayered bioadhesive buccal tablets. To the optimized formulation, 10mM sodium taurocholate was added to increase the permeation of poorly permeable ondansetron HCl. It is well known that natural surfactants like bile salts increase the permeability of drugs by perturbation of intercellular lipids. The results indicated that, from pure drug solution (5 mg/mL) about 88.63% cumulative percentage of drug permeated across porcine buccal mucosa with flux of 0.0235 mg.h-1cm-2. However, the optimized formulation with sodium taurocholate increased flux (0.0523 mg.h-1cm-2) and cumulative amount of drug permeated (65.44%) in comparison to formulation without permeation enhancer (38.45%, 0.0186 mg.h-1cm-2) with enhancement ratio of...
Read More
