Posted by admin on Jul 1, 2011 in |
Present study investigated the protective effect of apigenin on cell surface glycoconjugates abnormalities in 7, 12-dimethyldenz(a)anthracene(DMBA) induced hamster buccal pouch carcinogenesis. DMBA painting in the hamster buccal pouch three times per week for 14 weeks developed oral squamous cell carcinoma. The levels of glycoconjugates (Protein bound hexose, Hexosamine, Lipid bound sialic acid, Total sialic acid and Fucose) were analyzed by using specific colorimetric methods. The levels of glycoconjugates were significantly increased both in plasma and buccal mucosa in tumor bearing hamsters as compared to normal hamsters. Oral administration of apigenin at a dose of 2.5mg/kg bw significantly restored the status of glycoconjugates in DMBA treated hamsters. Our results suggest that apigenin protected cell surface glycoconjugates abnormalities during DMBA induced oral...
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Posted by admin on Jul 1, 2011 in |
The purpose of the current work was to conduct phytochemical screening and antioxidant activity of different extracts of Leucas aspera. The dried powder of the whole plant was extracted with n-hexane, ethyl acetate and ethanol which were subjected to various chemical tests to ascertain the main constituents of the plant. The results revealed the presence of significant amounts of alkaloids, glycosides, tannins and flavonoids in ethanol extract while the other two extracts contain moderate amount of the chemical constituents. Our results indicate the presence of phytochemicals in the order of ethanol > ethyl acetate > n-hexane extract. Antioxidant activity of these extracts were performed by using DPPH free radical scavenging assay, total anti-oxidant capacity and the total phenol capacity where ascorbic acid (for DPPH scavenging and total anti-oxidant) and gallic acid (for total phenol) were used as standards. The results showed that the ethanol extract possesses more anti-oxidant activity than ethyl acetate and n-hexane...
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Posted by admin on Jul 1, 2011 in |
In the present study, toxicity was developed by oral administration of AFB1 at a dose of (2µg/kg body weight) for 45 days in male mice. Curcuma longa (100 and 200 mg/kg body weight) and Curcumin (50 and 100 mg/kg body weight) were given individually and in combination with AFB1 for 45 days. At the end of 45 days, the blood samples were collected from various groups by heart puncture in eppendroff tubes with anticoagulant for hematological assays. Noncoagulated blood was tested for total erythrocyte count, total leukocyte count, hemoglobin, differential leukocyte count and for other blood indices. The results of present study suggest that Turmeric rhizome powder (Curcuma longa) and Curcumin showed protective effects against AFB1 induced toxicity by modulating red blood cell count, white blood cell count and Hb percentage to some...
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Posted by admin on Jul 1, 2011 in |
Buccholzia coriacea Engle (Capparidaceae ‘wonderful cola’) a tropical plant is used in traditional medicine in the management of diabetes, malaria and hypertension. This study aims to investigate the larvicidal activities of the Petroleum ether, chloroform fractions and methanol extract of B. coriacea seed as a potential agent in vector control for malaria. Third and fourth instar larvae of Anopheles gambiae were used for the study. Five doses (62.5 μg/ml, 125 μg/ml, 250 μg/ml, 500 μg/ml and 1000 μg/ml) of the methanol extract petroleum ether and chloroform fractions tested against the larva with 1% ethanol used as control. The mortality rates were determined 24 and 48 hr after exposure to extract following the WHO procedure. The chloroform fraction exhibited the highest mortality rate of 100% at 250, 500, 1000 μg/ml after 24 hr exposure, the pet ether exhibited mortality rate of 77.5% (1000 μg/ml) after 48 hr exposure while the methanol extract had the lowest effect of 22.5 % after 48 hr. This study reports for the first time the...
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Posted by admin on Jul 1, 2011 in |
The objective of the study is to prepare, characterize and evaluate starch phosphate, a new modified starch as a carrier in solid dispersions for enhancing the dissolution rate of ritonavir. The feasibility of formulating solid dispersions of ritonavir in starch phosphate into compressed tablets with enhanced dissolution rate was also investigated. Starch phosphate was prepared by reacting starch with di-sodium hydrogen orthophosphate anhydrous at elevated temperatures. It was insoluble in water and has good swelling (400%) property without pasting or gelling when heated in water. Solid dispersions of ritonavir in starch phosphate were prepared by solvent evaporation method employing various weight ratios of drug: starch phosphate such as 2:1(SD-1), 1:1(SD-2), 1:2(SD-3), 1:3(SD-4) and 1:9(SD-5) and were evaluated for dissolution rate and efficiency. All the solid dispersions prepared gave rapid and higher dissolution of ritonavir when compared to pure drug. A 58.34 and 94.41 fold increase in the dissolution rate (K1) of ritonavir was observed with solid dispersions SD-4 and SD-5 respectively. The DE30 was also increased from 6.80%...
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