Posted by admin on Mar 1, 2011 in |
The present study was aimed to establish a protocol for multiple shoot induction via the culture of cotyledonary nodes of Clitoria ternatea L., procured from 15 days old aseptic seedlings. Seeds were germinated on MS basal medium supplemented with gibberellic acid (1.0 μM) within one week of inoculation. Cotyledonary node of Clitoria ternatea L. gave rise to multiple shoots (number of shoots varied) when cultured on MS medium fortified with different concentrations of Benzyl adenine (BA) and Kinetin (Kn). The highest rate of shoot multiplication (16.04 ± 0.24) with 90% response was obtained on MS medium containing Benzyl adenine (5.0 μM). Regenerated and elongated shoots were rooted on half strength MS medium fortified with Indole 3-butyric acid (IBA) (2.0 μΜ) after two weeks of sub-culturing. Complete plantlets were then hardened, acclimatized and transplanted to natural conditions, where they exhibited 80% survivability. Hence, from this study, we report for the first time in vitro propagation from cotyledonary nodes collected from in vitro grown seedlings of Clitoria ternatea L. About 10...
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Posted by admin on Mar 1, 2011 in |
The objective of present study was to develop a co-processed diluent consisting of physically modified starch and dicalcium phosphate and to assess its suitability in direct compression. Native starch was exposed to nitric acid under controlled conditions. Dicalcium phosphate was incorporated in the neutralized aqueous starch dispersion to prepare co-processed excipient. The modification did not cause any detectable change in the shape and size although rupture was observed in few starch grains. The co-processed diluent was evaluated for particle size analysis, flowability and compressibility. Acetaminophen and glycyrrhiza glabra powder were chosen as poorly compressible model drugs. Nitric acid treatment resulted in formation of starch with high crystallinity. The modified starch and co-processed excipient showed acceptable angle of repose, Carr’s index, Hausner’s ratio and tapped density. The tablets showed acceptable crushing strength, disintegration and friability. Co-processed diluents exhibited improved flow and compressibility. Starch contributed to economy and disintegration while dicalcium phosphate contributed fragmentation propensity and insulation towards moisture....
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Posted by admin on Mar 1, 2011 in |
A simple, sensitive, high throughput and specific HPLC method with UV detection was developed and validated to according to the FDA guideline for quantification of gemifloxacin in human plasma. The bioanalytical method consists of liquid-liquid extraction and quantification by UV detection. The analyte was separated isocratically on a Cyano column (250 X 4.6 mm, 5µ particle size) with a mobile phase consist of 1% v/v formic acid, acetonitrile and methanol in the ratio of 75:20:5 (v/v). The response to gemifloxacin was linear over range 0.025 to 2.000 μg/mL. The validation results demonstrate that the method had satisfactory precession and accuracy across the calibration range. Intra-and inter-day precession range from 0.676 to 1.222 and from 0.834 to 1.645% (RSD), respectively. The accuracy determine at three quality control samples was within 94.232 to 101.132%. There was no evidence of instability of the analyte in human plasma following the stability study. The method proved highly reproducible and sensitive and was successfully applied in bioequivalence study after oral dosing of gemifloxacin tablet in...
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Posted by admin on Mar 1, 2011 in |
The objective of this study was to review and explore the top fifteen currently herbal remedies used and the historically used herbal remedies in the treatment of psoriasis. Psoriasis is undoubtedly distressing, affected individuals are typically otherwise healthy and thus well suited to thoughtful outpatient care. Recent advances in our understanding of psoriasis have provided parallel advances in topical treatments. Recent advances in our understanding of psoriasis have provided parallel advances in topical treatments. Specifically herbals, there is limited scientific data regarding their benefits and interactions. About 75% of patients have mild-to-moderate psoriasis, amenable to topical treatment is lifetime controlling herbals remedies like Aloe, Cayenne, Chamomile, Dong Quai, Emu oil, Evening prime rose oil, Fish oil, Tea tree oil, Turmeric, Slippery elm, Wintergreen, Shark cartilage, Milk thistle, Glucosamine, Flexseed oil are needed. Herbal remedies for treatment psoriasis diseases to overcome the adverse effect, antagonistic effect and bioavailability of...
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Posted by admin on Feb 1, 2011 in |
Microencapsulation is a well-known method that is used to modify and retard drug release. In this study, the preparation of Zidovudine loaded microcapsule was accomplished in order to assess the suitability of them for production of oral/vaginal sustained release multiparticulate dosage form of Zidovudine.Microcapsules were prepared using the solvent evaporation method. The effect of formulation variable like polymer: drug ratio was investigated on shape and surface characteristics of microcapsules (by scanning electron microscopy), percentage yield and drug entrapment efficiency, mean particle size and drug release profiles (dissolution test). Microscopic examination of microcapsules revealed that microcapsules were well-shaped and nearly spherical. FTIR studies revealed that no interactions were found between the drug and the polymer. The particle size of all the formulations were found to be satisfactory and within the range of 60.06 to 85.28 μm The percentage yield of all the formulation was found to be satisfactory except MC5. The percentage yield varied from 60.16 to 92.52% suggesting that the processing parameters did not affect the yield from the...
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