Posted by admin on Jan 1, 2011 in |
The aim of the study was to evaluate the circulating antioxidant such as vitamin C, vitamin E and GSH in nickel (Ni) induced toxicity in rats and in vitro free radical scavenging assay. In this investigation nickel sulfate (20 mg/kg body weight (b.w.) was administered intraperitoneally (i.p.) for 20 days to induce toxicity. Naringin was administered orally (80 mg/kg body weight) for 20 days with i.p. injection of nickel sulfate. The toxic effect of nickel was indicated by significantly decreased activities of non-enzymatic antioxidants like reduced glutathione, vitamin C and vitamin E. Treatment with naringin exhibited a significant (P < 0.05) increase in Ni-induced rats. The free radical scavenging properties of naringin were investigated with different in vitro methods such as 2, 2¢- diphenyl- 1-picrylhydrazyl radical (DPPH•), 2, 2’- azinobis (3-ethylbenzo thiazoline- 6- sulfonic acid) radical (ABTS•+), hydroxyl radical, superoxide anion scavenging activity and reducing power. In addition to that ascorbic acid, butylated hydroxyl toluene was used as the reference antioxidant radical scavenger compounds. Among the different concentration, 500...
Read More
Posted by admin on Jan 1, 2011 in |
Plants are the local heritage with global importance. World is endowed with a rich wealth of medicinal plants. They represent and alternative treatment of non-serve cases of infections diseases. They are also the essential an integral part in complementary and alternative medicine and due to they which have developed the ability for the formation of various phytochemicals like terpenoids, steroids, flavonoids, tannins, phenols, saponin, cardio glycosides, carbohydrates, alkaloids, amino acids and proteins substances which are in turn used to restore health and heal many diseases. Natural products of plant and animal origin offer vast resource of newer medicinal agents with potential in clinical use. The present study was an attempt to analyse the phytochemical analysis of Aegle marmelos, Ruta graveolens, Opuntia dellini, Euphorbia royleana, Euphorbia antiquorum infive different solvent extracts. Some of the plants extracts have shown presence of...
Read More
Posted by admin on Jan 1, 2011 in |
Antipyretic effect of petroleum ether and chloroform soluble fractions of ethanol extract of the roots of Vernonia cinerea was investigated. Intraperitoneal administration of boiled milk at a dose 0.5 ml/kg body weight in albino rabbit leads to pyrexia. Intraperitoneal (i.p. route) administration of petroleum ether and chloroform soluble fractions of ethanol extract of the roots of Vernonia cinerea at a dose 250 mg/kg body weight were shown significantly reduce the elevated body temperature of rabbit which was compared with standard aspirin (market product) and solvent...
Read More
Posted by admin on Jan 1, 2011 in |
Tylophora indica is a perennial climbing plant native to India, found in plains, forests, hills of southern and eastern India. The portions of plant used medicinally are leaves. The Total phenolic content of methanolic leaves extract was estimated by Folin-Ciocalteu assay method, and was found to be 0.160 mg/CE/g (Catechine Equivalent per gram). However, antioxidant activity of methanolic leaves extract of Tylophora indica was determined by DPPH free radical scavenging method. The DPPH radical scavenging activity of methanolic extract of Tylophora indica was found to be highest at 100μl concentration which was 30.74%. Nevertheless, % DPPH scavenging activity of standard ascorbic acid at same concentration was found to be 45.43%. The % DPPH scavenging activity increases with the increasing concentration. The concentration of Tylophora indica needed for 50% inhibition (IC50) was found to be 199.58μg/ml whereas 194.58 μg/ml needed for ascorbic...
Read More
Posted by admin on Jan 1, 2011 in |
A rapid and simple high performance thin layer chromatography (HPTLC) method with densitometry at 230 nm was developed and validated for simultaneous determination of Naproxen sodium and Sumatriptan succinate from pharmaceutical preparation. Separation was performed on aluminum-backed silica gel 60F254 HPTLC plates as stationary phase and using a mobile phase comprising of methanol:distilled water:formic acid in the volume ratio of 0.5:7.5:0.1 (v/v/v), respectively. After development, plates were observed under UV light. The detector response was linear in the range of 200-1200 ng/spot and 100-1000 ng/spot for Naproxen sodium and Sumatriptan succinate. The validated lowest limit of detection was 85 ng/spot and 40 ng/spot whereas lowest limit of quantification was 200 ng/spot and 100 ng/spot for Naproxen sodium and Sumatriptan succinate, respectively. The percentage assay of Naproxen sodium and Sumatriptan succinate was found between 99.25 and 98.03 % respectively. The described method has the advantage of being rapid and easy. Hence it can be applied for routine quality control analysis of Naproxen sodium and Sumatriptan succinate from pharmaceutical preparation and...
Read More
