Posted by admin on Jan 1, 2010 in |
A simple, sensitive and rapid high performance liquid chromatography method with UV detection (215nm) was developed and validated for the quantification of Levetiracetam in human plasma. Following a single-step liquid–liquid extraction with diethyl ether/dichloromethane (70/30 v/v), the analyte and internal standard (Zonisamide) were separated using an isocratic mobile phase, mixture of buffer (5mM Di-Potassium hydrogen phosphate anhydrous, pH7.2): Methanol (85:15 v/v) on reverse phase C-8 Kromasil column. The lower limit of quantization was 1µg/mL, with a relative standard deviation of less than 20%. A linear range of 1µg/mL to 40µg/mL was established. This HPLC method was validated with between and within-batch precision of 5.6–8.9% and 3.9-5.3%, respectively. The between and within batch accuracy was 99.9-106.3% and 96.2-102.0%, respectively. Frequently co-administered drugs did not interfere with the described methodology. Stability of Levetiracetam in plasma was >90%, with no evidence of degradation during sample processing (autosampler) and 60 days storage in a freezer. This validated method is sensitive and simple with between-batch precision of <10% and was used in pharmacokinetic...
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Posted by admin on Nov 1, 0203 in |
Lippia nodiflora L. is an important medicinal plant of Verbenaceae family and are used traditionally for several ailments. The present study deals with the detailed physicochemical, fluorescence and phytochemical evaluation of aerial parts of Lippia nodiflora L., in order to explore the authentic plant material suitably for its traditional claims. Thephysicochemical evaluations and fluorescence analysis were determined as per standard protocols. Phytochemical constituents of dried plant material were carried out by both qualitative and quantitative methods. Gas Chromatography–Mass Spectrometry (GC-MS) was used to analyze the phytochemical constituents of the methanol extract. Analysis of physicochemical parameters of the powdered aerial parts showed that total ash was approximately two times more than water insoluble ash and alcohol soluble extractive value was higher than water soluble extractive value.Preliminary phytochemical screening showed the positive result for the presence of flavonoids, steroids, glycosides, alkaloids, terpenoids, tannins and phenolics. Quantitative analysis showed that the methanolic extract consist of high phenolic compounds (98.31 ± 0.004 mg GAE/g) followed by total flavonoids (60.88 ±0.001mg QE/g) and flavonols...
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Posted by admin on Jun 1, 0203 in |
Objective: The aim of the present study is to investigate the synthesis and characterization of Silver nanoparticles (AgNPs), and their antimicrobial effect on gram positive and gram negative bacteria by using hydro alcoholic leaf extract of Ipomoea Aquatica Forsk (HAEIA). Material and Methods: AgNPs were prepared by green synthesis process from 1mM AgNO3 solution the aqueous silver ions when exposed to the HAEIA were reduced and stabilized over long periods of time resulting in the green synthesis of surface functionalized silver nanoparticles. The bio-reduced silver nanoparticles were appropriately characterized by using UV-Vis spectroscopy, Particle size distribution and Zeta potential. Antibacterial activity of synthesized silver nanoparticles (AgNPs) was carried out on both Gram negative and Gram positive bacteria and compared with standard antibiotic Ciprofloxacin. Result: The particle size of bio synthesized silver nanoparticles is found to be in the range of 77 – 624 nm with polydispersity index (PDI) of 0.207 clearly indicates that the particles are in very narrow size distribution. The zeta potential value of -6.16 mV that...
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Posted by admin on May 1, 0203 in |
Bioavailability / Bioequivalence (BA/BE) studies are conducted as a part of regulatory requirement to establish that the generic drugs are equivalent to the innovator drugs and are usually carried out in healthy human volunteers. They are nontherapeutic studies without any direct benefit to the participants. In spite of the lack of any direct personal benefit what makes some individuals to volunteer to take part in the study while others are not and what is their opinion about BA/BA studies is largely unknown. Hence the present study was conducted to find out the reasons for participation in BA/BE studies by healthy volunteers and the problems they encountered during the study participation. 112 healthy volunteers participated in this study and all of them were males. From their response, it is evident that 70 volunteers participated in BA/BE studies for monetary benefit. 64 volunteers were of the opinion that free health checkup they undergo in a BA/BE study helps to know their health status and they participated in the studies for this...
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Posted by admin on Dec 1, 0202 in |
The leaf extracts of Lantana camara in different organic solvents (methanol, acetone, ethanol and aqueous) were assessed in-vitro for fungitoxic activity against phytopathogenic Alternaria alternata isolated from potato (Solanum tuberosum) and tomato (Lycopersicon esculentum). The assessment of fungitoxicity was carried out by food poison technique1 using four different extracts at 200 mg/ml and their activity was recorded as radial growth and percentage inhibition. Among the four extracts, ethanol and acetone extracts showed complete inhibition of growth of fungus; while methanol extract showed 50% inhibition and aqueous extract did not produce any inhibition of fungus. Findings from present study confirmed that ethanol and acetone extract of Lantana camara can be used as biofungicide to control this phytopathogenic...
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