Posted by admin on Feb 28, 2022 in |
Background: Dengue is an arthropod-borne viral disease caused by the dengue virus (DENV). DENV is an RNA virus that belongs to the Flavivirus genus. The incidence of dengue has grown in the recent decade globally. The World Health Organization (WHO) considers dengue as a major global public health challenge in the tropic and sub-tropic nations. Materials and Methods: NS1 antigen and IgM antibody ELISA were done, and the serum levels of Vitamin-D and Mannose Binding Lectins (MBL) were determined to find out their association with the severity of Dengue, i.e., Dengue fever (DF) / Dengue Haemorrhagic Fever status (DHF). Results: The male-to-female ratio was 1.35:1 with the most commonly affected age group of 11-20 years (31.3%). NS1 antigen ELISA alone was positive in 12 (15%) samples, IgM ELISA alone was positive in 20 (25%) samples, while NS1 and IgM ELISA both were positive in 48 (60%) samples. 64 (80%) and 16 (20%) cases were DF and DHF, respectively. Conclusion: Thrombocytopenia is significantly notable in DHF patients as compared to...
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Posted by admin on Feb 28, 2022 in |
Background and Objectives: This study was carried out with the objective to investigate the antibacterial and antifungal activity of the bark of Hardwickia binata Roxb. Materials and Methods: Methanolic extract of bark of Hardwickia binata Roxb. exhibited an inhibitory effect towards the pathogenic organisms. Antimicrobial activity the zone of inhibition values of methanolic extract of bark of Hardwickia binata Roxb. in against two Gram-positive microorganisms viz. Staphytococcus aureus and Bacillus subtilis and two Gram negative microorganisms viz. Escherichia coli and Pseudomonas aeruginosa showed significant activity. Antifungal activity of methanolic extract of bark of Hardwickia binata Roxb. in against two microorganism, Candida albicans, and Aspergillus niger, showed significant activity. Results: The results showed that the remarkable inhibition of the bacterial growth was shown against the tested organisms. The phytochemical analyses of the plants were carried out. The microbial activity of the Hardwickia binata was due to the presence of various secondary metabolites. Conclusion: Hence, these plants can be used to discover bioactive natural products that may serve AS leads in...
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Posted by admin on Feb 28, 2022 in |
The aim of the present work is to develop and optimize solid lipid nanoparticles (SLNs) of pomalidomide with the aid of Box-Behnken design (BBD). A design space with three formulation variables at three levels was evaluated in BBD. Amount of lipid (A), surfactant (B), and co-surfactant (C) were selected as independent variables, whereas particle size (Y1), entrapment efficiency (Y2), and percentage drug release after 12 h (Y3) as responses. Pomalidomide-SLNs were prepared by hot emulsification/ ultra-sonication method and evaluated for responses to obtain optimized formulation. The effect of different levels of factors was evaluated for the particle size, PDI, zetapotential, entrapment efficiency, and % cumulative drug released. Kinetic model fitting for all pomalidomide SLN formulations was done to interpret the release rate. Characterization for the optimized formulation was done by FTIR, SEM, and stability studies. The optimized formulation PLF15 with 6% of tricapric as lipid, 6% of poloxamer 188 as surfactant and 2% eupikuron 200 of co-surfactant used in the nanoparticles with 126.59 ± 1.17 nm of size, 0.172...
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Posted by admin on Feb 28, 2022 in |
A simple, accurate, precise, reproducible, economical UV spectrophotometric method has been developed and validated for simultaneous estimation of Hydroxychloroquine sulfate (HCQ) and Nitazoxanide (NTZ) in a synthetic mixture. Method A by simultaneous equation method, Method B by the first-order derivative method. Method A: simultaneous equation method was based on the measurement of absorbance of nitazoxanide at 345 nm and Hydroxychloroquine Sulfate at 220 nm. Method B: first-order derivative method was based on the measurement of absorbance of HCQ measure at 226 nm (ZCP of NTZ) and absorbance of NTZ measure at 375.50 nm (ZCP of HCQ). The calibration curve was found to be linear in the concentration range 2-10 μg/ml and 5-25μg/ml for HCQ and NTZ, respectively (n=6), with their correlation coefficient of 0.999. The developed method was validated according to the ICH...
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Posted by admin on Feb 28, 2022 in |
The study was designed to study the macroscopic, microscopic, and also physicochemical parameters such as fluorescence analysis, extractive values, ash values, and loss on drying were performed. Therefore, this parameter is essentially used to control the quality of crude drugs and/or herbal drugs/drug products. The moisture content (% LOD) of the leaf powder was found to be 5.10± 0.14 (% w/w), total ash was found to be 12.27 ± 0.3 (% w/w). The amount of acid insoluble ash and water-soluble ash was found to be 14.5 ± 0.25, 6.23± 1.22 (% w/w), respectively. The extractive values of hexane extract were found to be 2.45± 0.26, ethyl acetate extract was found to be5.62±0.17, the water-soluble extractive value of the drug was found to be 9.142 ± 0.58 (% w/w), and the alcohol-soluble extractive value was found to be 14.64 ± 0.28 (% w/w). The results of physicochemical analyses lie within the acceptable limit, which in turn ascertains the quality and purity of leaf...
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