Posted by admin on Dec 31, 2024 in |
Background: Human Papilloma Virus (HPV) is a common sexually transmitted infection, and its persistence can lead to cervical cancer. It can be prevented by vaccines. Objectives: The study was conducted with the objective of analyzing knowledge, attitude, and practice towards HPV infection and the acceptability of vaccination among medical students. Methodology: Around 405 students in a tertiary care centre were randomly selected. Data were collected using a validated questionnaire and statistically analyzed using MS Excel and SPSS version 16. Results: Clinical students had significantly (p<0.01) better knowledge than non-clinical students in areas like HPV and cervical cancer (99%, 91%), sexual transmission (98%, 86%), and HPV vaccine (92%, 74%). Non-clinical students significantly (p<0.01) differ from clinical students by agreeing in areas related to vaccination, perceived as “sexually active” (32%, 22%), parent’s decision (40%, 25%) and partner’s opinion (81%, 56%). None of the students were ever screened for HPV infection or cervical cancer. No difference was seen between the phases related to HPV transmission through direct contact and non-curability. Conclusions: Clinical...
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Posted by admin on Nov 30, 2024 in |
Background- Depression is a mental health condition with various symptoms including low energy, disrupted sleep, and suicidal ideation. Antidepressants have negative side effects and can cause drug interactions. Curcuma longa, a herbal extract, has shown potential in treating depression due to its antioxidant and anti-inflammatory properties. A study is planned to evaluate its antidepressant activity. Aim and Objective: To study the anti-depressant activity of Curcuma longa rhizome using animal models and to find out its mechanism of action. Materials and Methods: Ethanolic extraction using Soxhlet apparatus, forced swimming test, open field behavior. Results: Curcuma longa rhizome possess antidepressant property. Conclusion: The study investigated the effectiveness of Curcuma longa rhizome in treating depression. Two doses of the ethanol extract were tested in mice, and their behaviour was assessed in various tests. The extract showed antidepressant action by increasing serotonin, norepinephrine and dopamine levels in the brain and antagonizing the GABA receptor. No sleep-promoting compounds were found in the...
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Posted by admin on Nov 30, 2024 in |
Artabotrys thomsonii is a medicinal plant used in the African pharmacopoeia for the treatment of various diseases. It was therefore important to assess its potential risk to human and animal health. This study highlights the acute and sub-acute effects of the toxicological profile of A. thomsonii leaves aqueous extract. The extract was prepared using the traditional protocol, then administered orally to Wistar rats at a single dose of 2000mg/kg for acute test, and of 100, 200 and 400mg/kg once a day for 28 days for sub-acute test. In acute toxicity, no behavioral disturbances were observed in treated animals compared with the control. The lethal dose 50 was estimated to be greater than 2000mg/kg and the extract was noted to possess low toxicity. Similarly, no significant changes in body mass, relative organ mass, hematological or biochemical parameters were observed after 28 days of repeated administration of the extract. Furthermore, administration of the extract did not alter motor coordination or develop anxiety in sub-acute treatment. Histological sections revealed that A. thomsonnii...
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Posted by admin on Nov 30, 2024 in |
Black rice is a rice species, Oryza sativa Linne (Gramineae), which is sticky, high in nutrients, and primarily grown in Asia. In recent years, the health benefits of black rice varieties have been reported due to the presence of a bioactive compound, anthocyanin, which has highly potent activity with high macronutrients. The present study aimed to evaluate and quantify the metabolites, focusing on describing the structure of Cyanidin-3-O-glucoside (CY3G) as a biomarker of anthocyanin present in the extract of black rice. Four different black rice varieties black rice extracts of Poireiton (BREP), Kokngangbi (BREK), Amubi (BREA), and Sempak (BRES) were collected, and phytochemical analyses performed using different standard methods. Compared to other black rice varieties, Amubi, BREA has the highest phenolic (63.23±0.76 mg/g GAE), flavonoid (28.09±0.68 mg/g QE), and anthocyanin (17.69±0.45 mg C/g DEW) content. It also exhibited the highest potent antioxidant activity, with IC50 values of 29.1 ± 1.11 mg/ml in the ABTS assay and 98.83 ± 0.61 mg/ml in the DPPH assay. The FTIR spectroscopy analysis result...
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Posted by admin on Nov 30, 2024 in |
This study focuses on optimizing the tissue culture of Aegle marmelos, a medicinal plant with diverse pharmacological properties. The research fine-tuned sterilization methods, identifying a 2% sodium hypochlorite solution as the most effective. Callus induction experiments revealed the optimal hormonal combination for Aegle marmelos as MS medium + Auxin-2,4-D (0.5 mg/l) + Cytokinin-BAP (1.5 mg/l). Suspension cell cultures were then developed as a foundation for further experiments. The study also explored permeabilizing agents (Triton X-100 and Tween-80) in Aegle marmelos cell cultures, finding that Tween-80 enhances the extraction of secondary metabolites more effectively than Triton X-100. High-Performance Liquid Chromatography (HPLC) analysis validated these outcomes. In conclusion, this research significantly contributes to optimizing plant tissue culture protocols for Aegle marmelos, advancing scientific understanding, and offering practical insights for improving the reproducibility and efficiency of Aegle marmelos cell cultures. These findings hold promise for potential publication, marking notable advancements in plant tissue culture and permeabilization...
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