Posted by admin on May 31, 2025 in |
The present research work is aimed to formulate and evaluate Nitrendipine nanosuspension (NTD-NS) and nanosuspension loaded oral fast dissolving film (NS-OFDF) to improve its low solubility and bioavailability. Wet media milling technique was employed to prepare NTD-NS, which was optimized and evaluated for different parameters. Poloxamer 188, Poloxamer 407, HPC, HPMC E5, HPMC E15, HPMC E50, HPMC K4M, PVP K30 were used for preparing various nanosuspension formulations. HPMC E15 and SLS were used to formulate optimized nanosuspension. NTD-OFDF was prepared using the optimized nanosuspension by solvent casting method. PEG 400 is added during film formation as plasticizers and HPMC is added as film former polymer. The NTD-OFDF were prepared, optimized and evaluated for different parameters. Optimized NTD-NS depicted particle size of 455.2 nm with polydispersity index (PDI) of 24.9% and zeta potential of -24.2 mV. Optimized NTD-OFDF exhibited surface pH of 7.12±0.0251, folding endurance of321±4, thickness of 0.171±0.003, disintegration time of 28.133±0.493 sec which was in the standard limits. The drug release from NTD-NS loaded OFDFs increased significantly, reaching...
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Posted by admin on May 31, 2025 in |
Antimicrobial resistance continues to cause antibiotics treatment failure and millions of deaths globally. Inhibition of a double-stranded binding protein-A enzyme (DsbA), machinery in bacteria resistance and pathogenesis mechanisms make the bacteria vulnerable to existing antibiotics, allowing restoration of antimicrobial efficacy. However, there is a gap in identifying bioactive and safe DsbA-inhibitors as a therapeutic restoration strategy. In this in-silico screening study, a co-crystallized E. coli-DsbA enzyme protein was used as the target. Seven bioactive ligands: linoleic acid, aloesin, azadirachtin, nimbin, palmitic acid, stearic acid, and aloeresin-A, showed maximum docking scores of low binding affinity (kcal/mol): -6.8345, – 5.9434, – 7.2810, -6.2394, -6.5958, -6.7170 and -6.9554 respectively, lower than the standard (9AG): -4. 6919. The ligands obeyed Lipinski’s rule of 5. The Root Mean Square Deviation (RMSD) of E. coli-DsbA complexes with azadirachtin, and aloeresin-A displayed remarkable stability throughout the simulations. Molecular dynamics simulation on azadirachtin and aloeresin-A showed great docking scores and modes over 10000ps and revealed lowest binding affinity scores, remain stable in the active site. Additionally, astructural-activity...
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Posted by admin on May 31, 2025 in |
Objective: The present investigation demonstrates a simple, sensitive and accurate high-pressure liquid chromatographic (HPLC) method for the determination of Alvimopan (AMP) in bulk and dosage form. The chromatographic separation was achieved within 10 minutes by using acetonitrile: potassium dihydrogen phosphate buffer pH 3.0 adjusted with orthophosphoric acid (50:50) as mobile phase on Altima Grace Smart C-18 column (5μ; 250 × 4.6 mm) at flow rate of 1.0 ml/min with injection volume 20µL at wavelength of 261nm. The retention time of the drug was found to be 5.02 min. This method was validated as per United States Food & Drug Administration (US-FDA) guidelines. The results of the validation parameters were found to be within the acceptance limits. The method was linear in the concentration range from 500-20000 ng/mL (r2 = 0.9998) and the accuracy of drug was found to be 95.83-104.63% for AMP. The lower limit of quantification was found to be ng/ml and the stability of recovered samples at different conditions was found to be more than 95%. The...
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Posted by admin on May 31, 2025 in |
Background: Breast cancer is the most occurring cancer in women and the second most reason for tumour related deaths in women. Chemoprevention, which involves employing dietary, natural, or synthetic items, has emerged as an intriguing strategy to deal with increase in Breast cancer cases. Plumeria pudica Jacq. is fast growing, having medium size and belongs to the family Apocynaceae. The high concentration of phenols and phenolic chemicals in P. Pudica suggests that it may be utilised to cure cancer. Materials and Methods: In present study, mammary carcinoma was induced in female wistar rats by intragastric (IG) administration of 7, 12-dimethylbenz[a] anthracene (DMBA) at a dose of 80mg/kg of body weight. Treatment of methanolic extract of plumeria pudica leaves (200mg/kg/BW, thrice a week) to breast tumor bearing rats was found to be effective against DMBA induced mammary carcinoma. Progression of mammary carcinoma associated with decrease in body weight, anaemia, leucocytopenia, elevated levels of tumorigenicity, increase in oxidative stress and increased Liver biomarker levels. Result: The study showed the ability of...
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Posted by admin on May 31, 2025 in |
The study provides a detailed morpho-anatomical description of Tonna dolium (Linnaeus, 1758), a commercially important tonnid gastropod, based on specimens collected from the Gulf of Mannar, India. The research examines the shell characteristics, head-foot morphology, mantle organs, visceral mass, reno-pericardial system, digestive system, reproductive system, and central nervous system of T. dolium through dissections and histological analysis. Key anatomical features are described and illustrated, including the proboscis structure, radula morphology, esophageal glands, and genital systems of both males and females. Comparisons are made with previously studied tonnid species, particularly Tonna galea and Tonna pennata. Notable distinctive features of T. dolium include the narrow gill and osphradium, radularrachidian with two pairs of secondary cusps, and confinement of seminal vesicles within the pallial oviduct. This comprehensive anatomical study contributes to a better understanding of tonnid taxonomy and provides insights into the adaptations of T. dolium for its predatory lifestyle in tropical marine...
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