Posted by admin on Feb 28, 2021 in |
Objective: Stability of In-vitro Diagnostics (IVDs) reagents was performed to check its quality standards, performance, and linearity. IVDs like Calcium reagent containing Arsenazo III were studied by Accelerated stability studies by considering temperature, pH, and light parameters. Materials and Methods: Stability data were obtained by using different instruments like UV spectrophotometer, Biochemistry analyzer, pH meter, HPLC, and HPTLC. This gives information about the degradation that occurred during storage, transportation, etc. Results: Calcium reagent containing Arsenazo III degrade 90.81% when placed at 42 °C by UV spectrophotometer analysis. The stability conditions’ effect on actual serum concentration was measured by taking reagent performance on a biochemistry analyzer. The linearity of reagents decreases at 42 °C and at normal temperature, linearity does not change. HPLC spectra gave degradation of reagent, which was analyzed by its retention time, peak height, and % area. Arsenazo III produces 91.25% remains undecomposed in 3 months when exposed to light. The shelf life of the calcium reagent was found to be 85.36. HPTLC spectra gave degradation of...
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Posted by admin on Feb 28, 2021 in |
Myrica esculenta Buch – Ham. ex D. Don has been used worldwide for the treatment of various ailments. The present work is an attempt to relate the presence of phytochemicals in leaves of M. esculenta with its antibacterial potential using seven different solvents for preparation of extracts. The preliminary phytochemical screening confirmed the presence of alkaloids, flavonoids, carbohydrates, tannins, vitamin C, fats and oils, terpenoids, and phenols. The total phenolic and flavonoid content in the examined plant extract was found to be 97.66 ± 0.16 mg GA/g and 51.3 ± 0.32 mg RE/g of leaf extract, respectively. The maximum zone of inhibition was recorded by aqueous extract at 22.66 mm against Clostridium perfringens followed by 15.66 mm against Shigella boydii. On comparing the antibacterial effect of various extracts, the methanolic and ethanolic extracts are not significantly different from each other in terms of inhibitory effect against the microbes under study. All the bacterial strains were found to be sensitive to at least two extracts of the plant, but the...
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Posted by admin on Feb 28, 2021 in |
Combretum mucronatum Schumach & Thonn is a scandent shrub whose leaves are widely used by Ghanaians and other West-African herbalists for the treatment of various infectious and non-infectious diseases. It is used to treat wounds, coughs, dysentery, worm infestation, neurological disorders and bacterial infections. Some coughs are as a result of mycobacterial infection. Despite the success gained in minimizing antibiotic resistance over the past decades, there still remains a need for new antibiotics. Due to the constant development of multi-drug-resistant bacteria to existing antibiotics, particularly those directed against multidrug-resistant Gram-negative bacteria such as the tuberculous bacteria, new antibiotics are needed. Folklorically used antimicrobial herbs are good sources of new antibiotics. In that regard, the aim of this study was to assess C. mucronatum leaves for its possible mycobactericidal activity and investigate its extracts phytochemically. The macroscopic, microscopic, and physicochemical characteristics were determined as preliminary means of quality assessment of the plant materials. Phytochemically, C. mucronatum is rich in a wide variety of tannins and flavonoids, and their corresponding TLC...
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Posted by admin on Feb 28, 2021 in |
Botanical name of coriander is Coriandrum sativum Linn. Coriandrum sativum belongs to the family Umbelliferae which is a highly reputed ayurvedic medicinal tree commonly known as Dhania. Essential oils, flavonoids, fatty acids, and sterols have been isolated from different parts of C. sativum so far. The phytochemical analysis of both methanol and AgNP’s extract was evaluated. Results revealed the presence of alkaloids, flavonoids, diterpenes, phenol, and phytosterol in the methanol extract, and AgNP’s extract contained flavonoids, phenols, and phytosterols. The antioxidant activity of both the extract was measured by the ability to scavenging DPPH, phenols, flavonoids, metal chelating, FRAP and was compared with suitable standards. These assays showed that both the extracts exhibit significant antioxidant activity. The highest percentage of inhibition was given by methanol extract when compared to standards, while in DPPH assay, AgNP’s showed the highest inhibition compared to methanol extract. The antibacterial activity of different concentrations of both extracts of C. sativum was determined against different bacterial strains. The methanol and AgNP’s extraction showed maximum inhibition...
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Posted by admin on Feb 28, 2021 in |
The waste generated from the textile industry is used for the production of biogas under the anaerobic digestion method. This study investigated the effect of various parameters such as Temperature 27 °C – 31 °C, pH 8-10, Inoculum concentration 35-55 ml on the textile dye with rice husk by the Agaricus heterocystis for biogas production using Response surface methodology. Maximum biogas yield 93 ml was obtained under the optimized condition of pH 9, Temperature 29 °C, and Inoculum volume 45 ml. The modified Gompertz model was used for maximum biogas production (1140 ml) from reactor 1 of textile dye (100 ml), rice husk (20 g), and inoculum volume (45 ml). The maximum biogas production (350 ml) was obtained under the optimized condition of Incubation time 144 h; Dye 100 ml/L and Rice husk 20 mg/L under the classical method. Produced biogas was confirmed and analyzed the composition: Methane – 78% and Carbon dioxide – 13% by Gas Chromatography (GC) method. The present study provided an efficient method for textile...
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