Posted by admin on Mar 31, 2021 in |
Ruellia tuberosa, commonly called Minnie root, has plenty of applications in herbal medicine due to the presence of several bioactive compounds. A simple, efficient, and reproducible in-vitro plant regeneration protocol was developed for Ruellia tuberosa. The cotyledonary nodal explants were excised from seedlings were inoculated on Murashige and Skoog (MS) medium supplemented with various concentrations of BAP and NAA, respectively. The highest numbers of 9.86 ± 0.15 shoots/explant were obtained in MS medium augmented with BAP (2.0 mg L-1) and NAA (1.0 mg L-1). The micro shoots induced per explant significantly increased with the supplementation of BAP to auxin-containing medium. The results showed that maximum percentage of multiple micro shoots 89%, with a number (13.80 ± 0.26) shoots / explant was found on MS medium supplemented with BAP (2.0 mg L-1) and NAA (1.5 mg L-1). Further, the highest frequency of micro shoots was produced on MS medium supplemented with TDZ (0.3 mg L-1) and AgNO3 (0.4 mg L-1) for large-scale production. The highest frequency of root induction (96%)...
Read More
Posted by admin on Mar 31, 2021 in |
Coronaviruses are a group of viruses that belongs to the Coronaviridae family. These viruses have helical symmetry, enveloped, positive-sense, single-stranded RNA genome with nucleocapsid that causes diseases in birds and mammals. SARS-CoV 2 complete genome has genome size of 29,903 bp, having nine genes identified as Orf1a Polyprotein, Orf1ab Polyprotein, ORF3a Protein, ORF7a Protein, ORF8 Protein, Envelope proteins, Spike Surface glycoprotein, Membrane glycoprotein, and Nucleocapsid Phosphoprotein. Based on the ProtParam result, ORF1a has more molecular weight (489989 daltons), and Envelope protein has a less molecular weight (8365 daltons). The pI is lower in Orf1a polyprotein, Orf1ab Polyprotein, ORF3a Protein, ORF8 Protein, and Spike Surface glycoprotein) and high in ORF7a Protein, Envelope proteins, Membrane glycoprotein, and Nucleocapsid Phosphoprotein. The Orf1a Poly-protein, Orf1ab Polyprotein, ORF3a Protein, Envelope proteins, Spike Surface, and Membrane glycoprotein are stable. The higher aliphatic index and more hydrophobicity of membrane glycoprotein suggest an increase in the thermostability of protein might favor an increase in its solubility. The vaccines like EIAV vaccine gp45, anti-SARS m396 Antibody, EIAV vaccine...
Read More
Posted by admin on Mar 31, 2021 in |
Objective: To assess the efficacy of Ma-ul-Sha’eer (Barley water) as a dietary intervention in Intermediate Hyperglycemic subjects. Methods: A hospital-based prospective, pre and post interventional studies, conducted on 40 diagnosed cases of Intermediate Hyperglycemia (Pre-diabetes) as per WHO and ADA criteria at OPD of Majeedia Unani Hospital and A & U Tibbia College and Hospital, Delhi. Results and Discussion: The efficacy of Ma-ul-Sha’eer (Barley water) was mainly observed on blood sugar fasting and HbA1c. The mean value of blood sugar fasting before the intervention was 113.6±6.7, and after the intervention was 103.0±5.4. Paired ‘t’ test was applied to the observations recorded before the intervention and after the intervention. It was observed that t=14.5 (p <0.0001) and were statistically highly significant. The most probable mechanism encountered in reducing blood sugar fasting may be the presence of β-glucan in Ma-ul-Sha’eer. The mean value of HbA1c before the intervention was 6.0±0.21 and after the intervention was 5.4±0.27, Paired‘t’ test was applied to the observations recorded before the intervention and after the intervention....
Read More
Posted by admin on Mar 31, 2021 in |
Medicinal plants are one of the most important resources for developing new lead therapeutic compounds used in various traditional medicines worldwide for thousands of years due to the presence of natural bioactive components, which help in preventing various human diseases. Piper betle Linn belongs to the Piperaceae family, commonly known as Betel and Paan. Betel leaves possess several bioactivities, and these are used in traditional medicinal systems. Syzygium aromaticum L. (Cloves) is a tree belonging to the Myrtaceae family. Closed flowering buds are used as a spice in food preparations all over the world. Eugenol is an active compound of both plants (Piper betle and Syzygium aromaticum). Hence, we attempted to study the effect of the methanolic extracts of Piper betle and Syzygium aromaticum, along with their active component, eugenol, using an in-vitro approach. The in-vitro cytotoxicity was assessed against lung adenocarcinoma cell lines (A549) using the MTT and SRB assays, and the type of cell death was characterized by differential staining. The results showed that the plant extracts,...
Read More
Posted by admin on Mar 31, 2021 in |
A simple, accurate, and economical, least time-consuming method has been developed for Losartan potassium (LP) by using UV spectrophotometer. This method was developed using phosphate buffer solution pH 7.4 (PBS) for quantifying the amount of LP released from the colon-specific formulations. The wavelength maximum (λmax) of LP in phosphate buffer pH 7.4 was found to be 228.5 nm. The developed UV spectroscopic method exhibited linearity in the range of 2-10 μg/mL with a correlation coefficient (R2) value of 0.999 and was validated with respect to linearity, specificity, accuracy (recovery), and precision. These parameters were determined according to International Conference on Harmonization (ICH) guidelines. Results of the analysis were validated statistically and by recovery studies, which proved the suitability of the developed method for the routine estimation of LP in colon-specific control release formulation. Hence, the reported method for the estimation of LP from the colon-specific formulations was simple, accurate, and least...
Read More
