Posted by admin on Mar 31, 2020 in |
Mating disruption by an artificial release of synthetic female pheromone components like Tetradecyl acetate (A), Ethyl (Z)-9-hexadecenoate (B) and a mixture of these two at different concentrations is a feasible method to control Xanthopimpla predator. Male Xanthopimpla predator was evaluated for responses to these synthetic pheromone components in a laboratory wind tunnel and in Terminalia arjuna field. Septa loaded with different concentrations (0.2, 0.4, 0.6, 0.8 and 1.0 mg) of Tetradecyl acetate (A), Ethyl (Z)-9-hexadecenoate (B), A and B blend in 1:1 ratio (C) has disrupted the male Xanthopimpla predator orientation in the wind tunnel and blend with 1.0 mg had shown more attraction towards Xanthopimpla predator. Mating disruption efficacy in tasar field was evaluated in first, second and third crops with 16 traps in each crop. In all crops, 5 traps baited with pheromone loads of 0.2, 0.4, 0.6, 0.8 and 1.0 mg of Tetradecyl acetate, 5 traps with Ethyl (Z)-9-hexadecenoate and 5traps with a blend of both in 1:1 ratio. One blank control trap without any type...
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Posted by admin on Mar 31, 2020 in |
A forced stability-indicating method for simultaneous estimation of glecaprevir and pibrentasvir by Ultra Performance Liquid Chromatography (UPLC) was developed and validated. The separation was done on column BEH C18 50 × 2.1mm 1.7m which was maintained at 30 °C, eluted with isocratic mobile phase and eluents were detected at 260nm. Mobile phase containing Buffer 0.01NKH2PO4: Acetonitrile taken in the ratio 50:50 was pumped through column at a flow rate of 0.3 ml/min. The retention time of glecaprevir and pibrentasvir was found to be 0.392 min and 0.607 min respectively. Validation with respect to specificity, system suitability, precision, accuracy, linearity, and robustness was performed as per ICH guidelines. % RSD of the glecaprevir and pibrentasvir were and found to be 0.5 and 0.7 respectively. % Recovery was obtained as 100.40 and 99.90% for glecaprevir and pibrentasvir respectively. LOD, LOQ values obtained from regression equations of glecaprevir and pibrentasvir were 0.79, 2.38 and 0.51, 1.55 respectively. Regression equation of glecaprevir is y = 2140x + 994.9 and of pibrentasvir was y...
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Posted by admin on Mar 31, 2020 in |
A new simple and sensitive liquid chromatography-electrospray ionization-tandem-mass spectrometric (LC-ESI-MS/MS) technique was developed and validated for the estimation of modafinil in human plasma and the method was applied to study pharmacokinetics in healthy rabbits. Chromatography was attained on Phenomenex- C18 (50mm × 4mm) 5µ column and acetonitrile, methanol and 0.1% formic acid (25:60:15 v/v) mixture as the movable phase at 0.7 ml/min flowrate. Modafinil and modafinil-D5 internal standards were detected at m/z 274.2/229.0, m/z 279.1/234.0 respectively. Modafinil and modafinil-D5 (internal standard) were separated with liquid-liquid extraction. The technique was linear over the 2.0-600.0 ng/ml concentration range. This technique established with intrabatch and inter batch precision within 1.54-7.18% and 1.82-6.25%. This technique established with intrabatch and inter batch accuracy within 98.56-102.80% and 97.62-102.76 %.The drug was shown mean Tmax of 3.833; average Cmax, AUC0͢ t and AUC0͢ α, for test formulation is 677.667; 6306 and 6471 respectively in the pharmacokinetic study on healthy...
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Posted by admin on Mar 31, 2020 in |
Nanoparticles are widely used in different technological fields, one of which is medicine. Collagen is used in several types of wound dressing. A silver nanoparticle is used as an antimicrobial agent to reduce microbial infection. In this research work, we have synthesized silver nanoparticles with collagen and studied for antibacterial activity against aureus (S. aureus), Escherichia coli (E. coli), and Pseudomonas aeruginosa (P. aeruginosa). The silver content and cell line study results reveal that the formulation contains less concentration of silver (0.109 µg /ml), and it is not toxic in 3T3 cell lines. Antibacterial activity of collagen-based silver nanoparticle formulation (F7) was found to be high against S. aureus, E. coli, P. aeruginosa when compared with silver nanoparticles...
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Posted by admin on Mar 31, 2020 in |
Background: Lumbar disc herniation is one of the commonest causes of low back pain. The magnetic resonance imaging is a gold standard non-invasive investigation for viewing lumbar anatomy in detail. Aim of the Study: To evaluate the effects of body mass index on the presence, extent and severity of lumbar disc bulging and herniation as well as to determine the relationship between clinical features and magnetic resonance imaging has proven lumbar disc bulging and herniation. Patients and Methods: A cross-sectional study conducted on 100 patients with lumbar disc bulging and herniation proved by MRI were included. Results: The correlation between body mass index and the total disc bulge/extrusion score was statistically significant. There was a significant association between neurological deficit and body mass index as well as between straight leg raising test, femoral stretch test, neurological deficit, and the waist to hip ratio. On the other hand, there was no significant association between clinical features and nerve root compression for patients with both normal body mass index as well...
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