Posted by admin on Apr 30, 2020 in |
Costus pictus D. Don., commonly known as the ‘Insulin plant’ is being used as a natural remedy to treat diabetes mellitus. The present study is focused to identify the chemical constituent responsible for the anti-diabetic activity of Costus pictus. The methanol extract was prepared from the fresh leaves of Costus pictus and washed with water to remove excess oxalic acid content present to get the non-toxic water-insoluble extract (CWI). The CWI was fractionated into low polar (CWI-LP), medium polar (CWI-MP) and high polar (CWI-HP) fractions by silica gel column chromatography. CWI and CWI-HP fraction showed prominent dose-dependent glucose tolerance effects in rats by GTT whereas the other fractions did not show any effect. Phytochemical analysis of CWI-HP resulted in isolation and characterization of the active compound present, namely β-Sitosterol-3-O-β-D-glucoside (daucosterol). The compound was purified by silica gel column chromatography and identified using various spectroscopic techniques, mainly through 1D and 2D NMR, HRMS. Since daucosterol is unstable in acidic pH, the enteric coating was performed on the molecule and CWI-HP...
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Posted by admin on Apr 30, 2020 in |
Last few decades, the remarkable advancement in the drug delivery system has been done; the oral route remains the importance and picks up the safest route of drug delivery. Regardless of striking advancements in the oral route medication, the current study focused on the formulation of a linagliptin buccal adhesive tablet. Formulated tablets are containing linagliptin as an active drug with a combination of different polymers such as carbopol (CP), eudragit RL-100 (EU), sodium alginate (SA) at different compositions with an impermeable backing layer of ethyl cellulose (EC). The formulation was carried out by direct compression, and tablets were evaluated by different parameters for pre and post-compression study. The post-compression evaluation parameters are weight variation test, hardness, thickness, friability, drug content, swelling index, pH followed by in-vitro drug release studies at pH 6.8. Compatibility study between drug-polymerr interactions was investigated by FTIR studies. Formulation F6, which contains a high concentration of EU provides maximum prolong the release of linagliptin among all other formulations. Formulation F6 has shown better control...
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Posted by admin on Apr 30, 2020 in |
The objective of this present work was to develop Solid Lipid Nanoparticle (SLN) gel for topical delivery of clobetasol propionate to enhance its permeation using silk sericin as a permeation enhancer. The proposed Clobetasol propionate gel loaded with solid lipid nanoparticle was prepared by solvent injection method. The effects of different strengths of lipid on the particle size, zeta potential, drug entrapment and release, and drug excipient interactions and the thermal behavior were evaluated. The final gel formulations were obtained by adding carbopol as a gelling agent and sericin a natural silk protein as a permeation enhancer and tested by an in-vitro gelling test. Prepared gel was evaluated for viscosity, pH, and in-vitro permeation properties. This study showed that the combination of carbopol with silk sericin produced the most promising gel formulation for topical application in the treatment of psoriasis. In this study, it was demonstrated that silk sericin-based gel, blended with solid lipid nanoparticle, could be produced with a cost-effective and industrially scalable technique. The outcome represents the...
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Posted by admin on Apr 30, 2020 in |
Predisposing exposures to cadmium have been implicated in hepatotoxicity, hence the search for therapeutic interventions. Thirty Wistar rats were randomly grouped into six (1-6) with 5 rats each. Group 1 received only 5mg/kg body weight (b.wt) of Cadmium Chloride (CdCl2) intra-peritoneally. Group 2 received feed and water ad libitum. Groups 3 and 4 received CdCl2 followed by low and high dose (10mg/kg b.wt and 40mg/kg b.wt respectively) of Aqueous Extract of the Zest of Citrus sinensis (AEZCs). Groups 5 and 6 were pre-treated with low and high doses of the extract before CdCl2. Extract administrations were orally and the experiment lasted for 8weeks. The livers were harvested, grossed and the liver to Bodyweight ratios (LW/BW) was determined shortly before being processed for histology and homogenate for SOD and MDA assay. Blood samples were collected for AST, ALT, ALP and total proteins (TP) analysis. Significant (p<0.05) differences in LW/BW, SOD, MDA, ALT, ALP, AST and TP were observed between groups 1 and 2. This was similarly noted between groups pre-treated...
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Posted by admin on Apr 30, 2020 in |
Consumption of fresh leafy vegetables by a street vendor cause salmonellosis is a major public health problem worldwide. The aim of the study to investigate total microbial count and the occurrence of ESBL producer and antimicrobial resistance pattern of Salmonella from fresh leafy greens. In this sample Salmonella spp. Population ranged from 40.33 to 35.33 × 102 CFU/g of fresh samples. The highest Salmonella spp. The population was observed in 40.33 × 102 CFU/g in green leaf, followed by Coriander and Ponnankanikirrai (red). Among the 10 isolates, five (50%) isolates namely NS-1, 2, 4, 5, and 7, could be ESBL producers by double-disc synergy test. From the 5 Salmonella spp., 4 isolates (88.89%) were resistance to cefuroxime and cefixime, 3 isolates (77.78%) resistant to ceftazidime and cefdinir, 2 isolates (33.33%) resistant to amoxiclav ceftriaxone and co-trimoxazole, and 1 isolate (11.11%) resistant to cefixime, amikacin, and chloramphenicol. Citric acid eradicated the Salmonella spp. averagely 100% at 3% concentration while 9.77 (0.5%) to 93% (2.5% concentration) from control. Acetic acid (3...
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