Posted by admin on Oct 31, 2019 in |
Tuberculosis is a global problem. The tuberculosis is the common cause of male infertility in developing countries due to the use of second-line anti-tuberculosis drugs (ATB). The experiments were conducted at Unique Bio diagnostic Enterprise Veterinary Pathology Laboratory Parel, Mumbai for chemiluminescence immunoassay to study the levels of testosterone in male Sprague-Dawley rats. The histological analysis was also carried out to confirm the study. From the study it was found that the body weights are inversely proportional to the weight of the testis. It was also found that Ethionamide administered rats showed lowest levels of testosterone, which may lead to infertility. To increase fertility potential we have administered Piper nigrum (Linn.) (PnS) seed ethanolic extract in combination with Ethionamide (ETH) + Para aminosalicylic acid (PAS) drugs. From the above results it is found that Piper nigrum increases the level of testosterone by maintaining the fertility potential in male Sprague-Dawley rats, it is also confirmed that the histological reconciliation of testis was appreciably restored in Sprague-Dawley rats administered with Piper...
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Posted by admin on Oct 31, 2019 in |
Nigella sativa is considered a miracle herb plant belongs to the Ranunculaceae family, inhabitant to South Asia and South-west Asia. The most important part of the Nigella sativa plant used by human beings are seeds, mostly as a spice because it contains a specific aroma. The seeds derived from Nigella sativa being exploited by people and used for thousands of years for culinary purposes, to cure various digestive disorders, recover from joint pains, obesity, common cold, headache, asthma, rheumatic diseases, etc. in India, Pakistan, Bangladesh, China, and Middle East countries. In the current study, the seed extracts of Nigella sativa were targeted for evaluation of qualitative biochemical analysis of phytochemicals, antibacterial, cytotoxicity, and antioxidant properties. Nanoparticles formulations derived as of the aqueous extract of seeds of Nigella sativa were developed and tested against selected microorganisms. FTIR and HPLC also performed to evaluate the functional group analysis and to detect the presence of a bioactive compound in the phytochemical extract. Cytotoxicity properties of methanolic and ethanolic seed extracts of Nigella...
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Posted by admin on Oct 31, 2019 in |
A simple, precise, rapid and accurate reverse phase HPLC method was developed for the simultaneous estimation of cobicistat and elvitegravir in the pharmaceutical dosage form. A column of ODS (250mm 4.6mm; i.d and 5µ particle size) was used along with the mobile phase comprising of 0.02M dipotassium hydrogen orthophosphate buffer (pH adjusted to 3.3) and methanol in the ratio of 80:20 (v/v). The flow rate was maintained at 1.0 ml/min and the effluents monitored at 254 nm. The retention time for cobicistat was found to be 2.58 ± 0.3 min and elvitegravir was 3.71 ± 0.3 min. The detection concentration was linear over 125-750 µg/ml for cobicistat and 12.5-75 µg/ml for elvitegravir. Regression equations of cobicistat and elvitegravir were found to be y = 25883x + 19711 and y = 27696x + 6046 respectively with regression co-efficient 0.999. The % RSD for Intra and Inter day precision was < 2%. The accuracy of method was validated by recovery studies and found to be significant within acceptable range 98-102%. The developed...
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Posted by admin on Oct 31, 2019 in |
Metal (II) complexes of Co, Ni and Cu with Schiff base derived from 2, 4-Dihydroxy-5-acetylacetophenone and Aminothiophenol (DAAAP) were synthesized and characterized by elemental analysis, FTIR, IH NMR, electronic spectra, thermal analysis, magnetic susceptibility and conductometric measurements. On the basis of these studies, a six coordinated octahedral geometry has been proposed for all the complexes. The Schiff base and its complexes were tested for biological activity against various types of Gram-positive (Bacillus subtilis ATTC 6051 and S. pyogones ATTC 12600) and Gram – negative bacteria (Escherichia coli ATTC 11775 and Proteus vulgaris ATTC 13315) and Fusarium solani Martius and Aspergillus niger Fungus. It has been found that metal complexes are found to have sensitivity for inhibition of Gram‐positive more than Gram‐negative bacteria. All the metal complexes show antifungal activity and it is clear from the data that the complexes exhibit more antifungal activity compared to the parent...
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Posted by admin on Oct 31, 2019 in |
Objective: To identify and quantify oleanolic acid from the roots of cyclea peltata by HPTLC method. Methods: The Plant cyclea peltata is a perennial herb from Menispermacae family. The powdered roots of plant cyclea peltata were extracted with methanol. Methanolic extract prepared separated with n-butanol, chloroform, and ethyl acetate as total saponin, total alkaloid and phenolic compound fractions respectively. The chemical characterization of oleanolic acid was done by High-Performance Thin Layer Chromatography (HPTLC) in a mixture of toluene: methanol (9:1) as a mobile phase which developed in anisaldehyde sulphuric acid reagent. Result: The extraction yield of total saponin fraction was 4.3% (w/w). The Rf value 0.31 found to same as the value of standard oleanolic acid. Linear regression analysis data for the calibration plots showed a good linear relationship between concentration (1-6 μg) R (r2) = 0.992408 concerning peak height and area. The limit of detection and limit of quantification of Oleanolic acid were detected to be 10.75 μg and 40.70 μg per spot. The amount of oleanolic acid...
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