Posted by admin on Oct 31, 2018 in |
Cajanus cajan L. (Pigeonpea) is an important pulse crop of India. The aim of this study was to determine and compare the antioxidant activity and bioactive compounds of seed coat and cotyledon of C. cajan. The antioxidant properties of pet ether, chloroform, ethanol and aqueous extracts of the seed coat and cotyledon of C. cajan were examined by a ferric thiocynate (FTC) assay, DPPH radical scavenging assay, hydroxyl radical scavenging assay and ferric reducing power were analyzed separately for its inhibition percentage at different concentration (20 µg, 40 µg, 60 µg, 80 µg and 100 µg/ml). Both the samples possessed antioxidant activity however the seed coat ethanolic extract possessed prominent activity by FTC (68.2 ± 0.07%), DPPH (90.1 ± 0.17%) and hydroxyl radical scavenging assay (75.2 ± 0.05%) at 100 µg/ml compared to other extracts. It was interesting to note that ferric reducing power of ethanolic extract of seed coat (1.590 ± 0.002%) was significantly (P<0.5) higher than that of standard BHA (1.575 ± 0.015%) at 100 µg/ml. The...
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Posted by admin on Oct 31, 2018 in |
Investigation of antioxidant, anti-inflammatory and antimicrobial activity of a dental film, which easily reabsorbs with applied phytocomplex of Geranium palustre, has been performed to use it for the treatment of oral mucosa diseases. To get the extract, the dried material was grounded, extracted and lyophilized. Quantitative determination of biologically active substances in lyophilized phytocomplex was performed on spectrophotometer and high performance liquid chromatograph. For investigation of antioxidant properties of the extract, the method, based on the interaction with stable chromogen-radical DPPH, was used. Investigation of anti-inflammatory activity of a dental film with extract of Geranium palustre herb was carried out based on carrageenin model of inflammatory. The study of antimicrobial properties of extract was performed using laboratory strains of microorganisms as a test-culture. The obtained qualitative indices of biologically active substances of the investigated phytocomplex showed high content of tannins, polyphenol compounds, polysaccharides, phenolcarboxylic acids, organic acids, ascorbic acid, free amino acids and other compounds, due to which lyophilized extract possesses a wide range of properties, important for treatment:...
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Posted by admin on Oct 31, 2018 in |
Organophosphate pesticides are associated with neurotoxicity. Organophosphates are cholinesterase-inhibiting chemicals used predominately as pesticides. These have played a very important role in sustainable production of food, animal feed and as protection against disease vectors. They are also used as chemical warfare agents (nerve agents). The effects of dichlorvos and monocrotophos on brain neurotransmitters are well established. The combined effect of organophosphates (dichlorvos and monocrotophos) was supposed to have synergistic or additive effect on brain neurotransmitters. Combined exposure of dichlorvos and monocrotophos has significant inhibition in acetylcholine esterase activity during both studies of 15 days and 30 days. Levels of nor-epinephrine, dopamine and serotonin (5-hydroxytryptamine) increased significantly in dichlorvos and monocrotophos exposed animals. However, the increase was more pronounced in monocrotophos alone exposed animals. Thus, contrary to hypothesis, the combined exposure to these toxicants did not produce more pronounced toxicity as compared to their individual exposure except in case of...
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Posted by admin on Oct 31, 2018 in |
A RP-HPLC method was developed for estimation of rosuvastatin calcium and validated as per ICH guidelines. The estimation was carried out on C-18 Reliant (4.6 × 250 mm, 5 µm) column using a filtered and degassed mixture of acetonitrile: phosphate buffer pH 2.6 (in the ratio 70:30) as the mobile phase at a flow rate of 1.0 ml/min. The detection was carried out at wavelength of 241 nm. The method was validated for linearity, specificity, accuracy, precision, sensitivity and robustness as per ICH norms. The retention time for the drug was 3.35 min. The method produced linear responses in the concentration range of 2-20 μg/ml with correlation coefficient (r2) of 0.998. The method developed was found to be simple, rapid, precise accurate and reproducible and can be used for routine analysis of rosuvastatin calcium. Diffusion procedure was performed using pig ear skin as diffusion barrier to characterize rosuvastatin calcium release rate from drug -in -adhesive patch and further analyzed by developed RP–HPLC method. The validated method was found to...
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Posted by admin on Oct 31, 2018 in |
Fagopyrum esculentum Moench. is a versatile herb as beyond its nutritional properties it also have various health benefits like anti-diabetic, anti-inflammation, anticancer, antibacterial, anti hypertensive etc. Most of these activities can be attributed to the presence of certain bioactive components categorised as polyphenols, present in its various parts. Present work describes a method for simultaneous detection and quantification of polyphenols present in the seed extract using HPLC and analyzing in-vitro anti-diabetic and anti-inflammatory potential of the extract. HPLC method was developed using Ultra HPLC with attached PDA detector, at a wavelength of 275 nm. The mobile phase used was acetonitrile: formic acid at gradient flow. Five polyphenols rutin, quercetin, apigenin, gallic acid, and catechin have been detected and quantified. The IC50 value (mg/mL; the concentration that causes a decrease in initial concentration by 50%) of ethyl acetate extract determined for α-Amylase, α-Glucosidase, and lipoxygenase enzymes was 471 mg/ml, 551 mg/ml and 524 mg/ml...
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