Posted by admin on Dec 31, 2018 in |
Nanoparticles speak to one of the appealing choices in the compelling treatment of tumor chemo-treatment. In the present work, definition, and improvement of a novel Cetuximab (MTX)- stacked biodegradable nanoparticles utilizing poly(D, L-lactide-co-glycolide) (PLGA) was done. Nanoparticles stacked with anticancer operators can effectively build sedate focus in malignancy tissues and furthermore act at the cell level, improving antitumor viability. These medication conveyance frameworks enhance bioavailability by improving fluid solvency, expanding protection time in the body and focusing on medication to a particular area in the body. The arranged nanoparticles were assessed for physicochemical properties, for example, molecule measure, zeta potential, discharge thinks about, and so forth. Molecule size of the upgraded definition was < 200 nm. Our essential outcomes exhibit that the created Cetuximab-stacked PLGA nanoparticles are discharging the medication for the delayed timeframe. The goal behind the work is to contemplate the impact of definition factors on the molecule estimate, sedate exemplification and % aggregate medication arrival of...
Read More
Posted by admin on Dec 31, 2018 in |
In this study, we investigated anti-cancer and antimicrobial activity of Aegle marmelos leaf extracts and their chemical profile characterized by gas chromatography coupled mass spectrometry (GC-MS). A. marmelos leaves were extracted with acetone, methanol, ethanol, and chloroform. Presence of phenolic compounds was identified in these extracts by qualitative analysis. All the extracts were subjected for anti-bacterial activity against the different strains of bacteria (Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Bacillus ariyabattai, Bacillus megaterium, Pseudomonas putida, Klebsiella pneumonia, Serratia marcescens, and Escherichia coli). It is noteworthy that acetone extract elicited maximum growth inhibition on Serratia marcescens. Based on profound anti bacterial activity, acetone and methanol extract of A. marmelos were checked for cytotoxicity against MDA-MB-231, HEp-2 and vero cells. MDA-MB-231 cells were more sensitive to acetone extract of A. marmelos with an IC50 value of 79.62 µg/ml where as HEp-2 cells are more sensitive to methanol extract of A. marmelos with an IC50 value of 47.08 µg/ml. Vero cells withstand 24 h treatment of both extract, and it is evidenced...
Read More
Posted by admin on Dec 31, 2018 in |
Aztreonam, a synthetic beta-lactam antibiotic is widely used for the treatment of infections in lungs, meninges, bladder, etc. Its potential use in the treatment of pneumonia and cystic fibrosis has been established. Recently FDA has approved Cayston® for inhalation therapy of Aztreonam in lungs infection. Many studies have been reported on the quantitative determination of Aztreonam by UV spectrophotometric and HPLC method. The present study focuses on the quantitative spectrophotometric determination of Aztreonam in two simulated lungs fluid namely artificial lysosomal fluid (ALF) and gamble solution. The λmax was found at a wavelength of 293 nm for both the solutions. Further, the method developed was validated for its linearity, precision by interday and within day study, accuracy, specificity, robustness and determination of limit of quantification and limit of detection in both the solutions. The linearity demonstrated a correlation coefficient of 0.9995 and 0.9999 in ALF and gamble solution respectively. The LOD was found to be 0.38 µg/ml and 0.18 µg/ml for ALF and gamble solution respectively. The LOQ was...
Read More
Posted by admin on Dec 31, 2018 in |
The increasing failures of chemotherapeutics and antibiotic resistance exhibited by pathogenic microbes have led to the screening of several medicinal plants for their potential antimicrobial activity. Thus, the present endeavour deals with the exploration of antibacterial efficacy of Curcuma caesia (Roxb.) (Family Zingiberaceae) against bacterial cultures procured from IMTECH, Chandigarh as a screen for potential candidates for the development of natural antibiotics. The agar well diffusion assay was performed to screen antibacterial activity, and ZOI was recorded. The results were analyzed using one way ANOVA with DMRT. A broth macro-dilution assay was used to determine (MIC) and (MBC). Finally, the purified fraction was chemically characterized by spectral analysis. The antibacterial activity profile revealed that the methanolic root extract exhibited broad-spectrum antibacterial potentiality. The extract exhibiting the highest antibacterial activity was purified by column chromatography and further with HPLC, and TLC analyzed the band pattern. The bio-efficacy of the purified fraction was assessed against pathogenic bacteria, and the MIC and MBC were determined. The maximum zone of inhibition was recorded...
Read More
Posted by admin on Dec 31, 2018 in |
The control of mosquitoes, which transmit deadly diseases, has become a significant public health concern globally. Protection against mosquito bites is an important part of preventing mosquito-borne diseases. Citronella oil extracted from two types of citronella grass Cymbopogon winterianus Jowitt (Java type) and Cymbopogon nardus (L.) Rendle (Ceylon type) possess compounds responsible for mosquito repellent activity. In the Sri Lankan market, there are some mosquito repellent products manufactured using citronella oil. In the present study, commercial citronella oil samples were subjected to GC-MS analyses weekly for sixteen weeks to investigate the variation in the percentage of compounds responsible for mosquito repellent activity with time and to correlate this variation to mosquito repellent efficacy of commercial citronella oil samples and sprays. In the tested samples, the percentages of compounds responsible for mosquito repellent activity as well as their mosquito repellent activity have shown a decline with time. The time duration that the maximum mosquito repellent activity would persist in the tested samples may be considerably lower than the shelf life...
Read More
