Posted by admin on Jun 30, 2018 in |
The present study aimed to develop and validate stability indicating method for the simultaneous determination of Elbasvir and Grazoprevir in its pharmaceutical dosage form using RP-HPLC. Chromatographic separation was done with Discovery C18 (250 mm × 4.6 mm, 5 µ) column using 0.1% ortho-phosphoric acid (OPA) and acetonitrile in the ratio 50:50% v/v as mobile phase on isocratic mode. The column oven temperature was maintained at 30 ºC with a flow rate 1.0 ml/min and components were detected at a wavelength of 315 nm. The retention times for Elbasvir and Grazoprevir was found to be 2.24 min and 3.21 min respectively. The developed method was validated according to ICH guidelines. A good linearity response was observed in the concentration range of 12.5 µg/ml – 75 µg/ml for Elbasvir and 25 µg/ml – 150 µg/ml for Grazoprevir with correlation coefficient of 0.999 for both the drugs. The method was found to be accurate, precise, specific, rugged and robust. The drugs were subjected to stress conditions for testing their stability and...
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Posted by admin on Jun 30, 2018 in |
ABSTRACT: Background: Postoperative pain control is an important part of effective postoperative care. However, 30 – 80% of postoperative patients complain about moderate to severe post-surgical pain as a result of the surgical procedure or a pre-existing disease. Aim: To assess the safety and efficacy of different modalities of postoperative pain management in post surgical wards of a tertiary care hospital. Method: It’s a prospective study was conducted in 213 patients of either gender, aged between 18 – 65 years. Patients were followed up from surgery till discharge or till the 4th postoperative day, the drugs given for pain relief both as single drug and combination drugs and adverse effects were monitored. The severity of pain was assessed using a numeric pain rating scale after 1 h of surgery, then followed by 2 h, 4 h, 6 h, 12 h, 24 h, 48 h and 96 h. Patient satisfaction with the analgesic therapy given was recorded at the time of discharge or after 4th postoperative day using 5 point...
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Posted by admin on Jun 30, 2018 in |
Background: It becomes clear that living processes were based on the action of enzymes. Cell immobilization serves as a useful tool for the immobilization of intracellular enzymes. The purpose of study is to improve economically important processes by attaching the yeast cells to matrices. Methods: In this analytical study by using mixed models of matrices like (sodium alginate alone, sodium alginate and gelatin, sodium alginate and agar, sodium alginate and glutaraldehyde) to determine the effective combination for immobilizing cells without any damage. Results: It was noted that the glucose content in both cells increased which indicates the production of carbon dioxide and alcohol and then the values started falling down this is because the osmotic concentration of the sugar gets so great that the yeast is unable to get enough water for growth. Conclusion: Glucose utilization was analyzed in free and immobilized cells on four different matrices for verifying their performance and the improvement of immobilized yeast cells over free yeast cells. Use of mixed matrices enhanced the biological...
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Posted by admin on Jun 30, 2018 in |
Lannea coromandelica is one of the important herbal plants well distributed in India. It is commonly called as Odiar, Gumphini in South India. It was extensively used by the tribes all over India to treat various diseases such as body pain, tooth ache, stomach ache, ulcers, sexual impotency. The aim of the present study was to evaluate the GC-MS analysis, antibacterial and anticancer activities of methanol bark extract of L. coromandelica. The methanol extract was subjected to GC-MS analysis to find out active volatile and semi-volatile compounds. The antibacterial activity was carried out by well diffusion method by using nutrient broth agar medium. Microorganisms such as Escherichia coli, Staphylococscus aureus, and Bascilus subtilis were used to evaluate antibacterial activity. The maximum zone of inhibition 21 mm was measured for Bascilus subtilis at 500 µg/mL concentration followed by Staphylococscus aureus was 19 mm and Escherichia coli was 18 mm. The anticancer activity was carried out by MTT assay method for MCF7 cell line. The maximum cell death was 74.20 ±...
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Posted by admin on Jun 30, 2018 in |
Objective: The persistence of this effort was to frame, evaluate and examine the impact of natural and semi-synthetic polymer in changing the release rates of matrix tablet loaded with diclofenac sodium. Materials and method: Matrix tablets of diclofenac sodium were made-up by varying the concentrations of both natural polymer i.e Guar gum and semi-synthetic polymer i.e HPMC K4M via straight compression method. Optimization of the tablet was completed by taking quantity of Guar gum and HPMC K4M in percentage as self-governing variable and cumulative percent of drug release at 2 hr, 6 hr and 12 hr as faithful variable using design expert Software. Optimized tablet was assessed for various pre and post compression limitations equated with marketed formulation. Drug release kinetics of the optimized tablet was evaluated by various representations of drug release kinetics. Result: The verdict of present investigation evidently designates that by using both natural and semi-synthetic polymer in a same tablet was given a noteworthy sustained release. The dissimilarity with the optimized formulation in software data...
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