Posted by admin on Feb 28, 2026 in |
The purpose of this research was to examine the harmful effects of Organophosphate (OP) on reproduction as well as the putative antioxidant agent Buchanania lanzan Oil (BLO potential)’s protective properties. A total of 32 male Wistar rats were divided into four groups of eight each: the control group (CRL), the Organophosphate group (OP, 27 mg/kg), the Buchanania lanzan oil group (BLO, 0.5 ml/kg), and the combination group (OP+ BLO). For 45 consecutive days, the test animals received gavage doses (five days per week). We looked at body weight gain, weights of the reproductive organs, sperm characteristics, testosterone levels, and levels of thiobarbituric acid-reactive substances (TBARS). According to the data, OP, considerably (p<0.001) reduced both the rate of body weight gain and the absolute weights of reproductive organs (testes, epididymis, and seminal vesicles). Moreover, significant modifications in semen at least (p< 0.01) A drop in spermatid number, sperm count, sperm motility, and testosterone level, with an increase in aberrant and dead sperm and TBARS level were seen in the OP,...
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Posted by admin on Feb 28, 2026 in |
Electrolytes are crucial for establishing and maintaining the electrical potential across cell membranes. Electrolyte abnormalities can precipitate life-threatening events. The incidence of electrolyte disorders is nearly 25% in ICU patients. Ion selective electrodes (ISEs) are so far commonly used method for electrolytes estimation in clinical laboratories. Studies using different devices have reported different results. Consequently, determining how well different methods align in their electrolyte readings is important. Comparisons of electrolyte results from arterial (ABG analyzer) and serum (electrolyte analyzer) samples, both utilizing the direct, non-diluting ISE method, have been the subject in this study. The objective to investigate whether the results of electrolytes measures on electrolyte analyser and ABG analyser are equal when used to assess electrolyte levels (sodium, potassium & chloride). The gap is usually small (1-4 mmol/L) but in critically ill patients or in sever hyperlipidemia / hyperprotenemia; the difference can be clinically significant. our study we concluded that if there is a large difference between the two methods in the same patient, one should check for...
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Posted by admin on Feb 28, 2026 in |
Background: Pancreatic ductal adenocarcinoma (PDAC) remains one of the most aggressive and treatment-resistant cancers, often driven by aberrant KRAS signalling. Kruppel-like factor 5 (KLF5), a downstream transcription factor in this pathway, has emerged as a potential therapeutic target. Chamazulene, a bioactive sesquiterpene derived from Matricaria chamomilla, known for its anti-inflammatory and antioxidant properties, but its anticancer potential remains unexplored. Objective: To evaluate the drug-likeness, toxicity, and KLF5-targeting potential of Chamazulene using an integrated in-silico approach. Methods: Chamazulene’s physicochemical and pharmacokinetic properties were assessed via SwissADME and PreADMET tools. Toxicity predictions were obtained using the GUSAR server. Molecular docking against homology-modelled KLF5 was performed using AutoDock Vina, and binding interactions were visualized. The top-scoring complex was further analyzed through 100-nanosecond molecular dynamics simulation using Desmond to assess structural stability under physiological conditions. Results: Chamazulene demonstrated favorable drug-likeness, high gastrointestinal absorption, BBB permeability, and a bioavailability score of 0.55. It showed no predicted hepatotoxicity or mutagenicity and had a high lethal dose (LD₅₀) value (>5000 mg/kg), although carcinogenic potential was flagged....
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Posted by admin on Feb 28, 2026 in |
Mobile health (mHealth) applications are increasingly used to support adherence by offering reminders, tracking tools and educational features. This narrative review aims to provide a user-centered comparative assessment of commonly available mHealth apps for medication adherence. This study was conducted over a period of 6 months to use and review six mobile apps. Each app was assessed based on eight key parameters such as user interface and navigation, reminder system, medication tracking, adherence support features, data security, customization options, integration and backup capabilities and technical stability. The analysis was based on direct app usage and user experience. My Therapy and Medisafe performed strongly across most parameters offering intuitive interfaces, flexible reminders, robust medication tracking and high data security. Medication Reminder and Alarm & Pill Reminder provided basic functionality with limited advanced features. Pillo, though visually engaging, lacked clinical depth and adherence tracking. Customization and integration features varied widely among the apps. mHealth applications offer valuable support in improving medication adherence. However, their effectiveness is influenced by the depth of...
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Posted by admin on Feb 28, 2026 in |
Objectives: CYP2C19 is an important enzyme involved in the metabolism of drugs such as proton pump inhibitors, antiplatelets, and antiepileptics. Genetic variations in CYP2C19 can alter drug response and safety. This study aimed to analyze the distribution of three key CYP2C19 variants (*2, *3, *17) and predict metabolizer phenotypes across South Asian, European, and East Asian populations using in-silico methods. Methodology: Allele frequencies were obtained from gnomAD and the 1000 Genomes Project. Genotype frequencies were estimated using Hardy–Weinberg equilibrium, and metabolizer phenotypes poor, intermediate, normal, rapid, and ultrarapid were assigned. Data processing and visualization were performed using R (version 4.5.1). Results: The *2 loss-of-function allele was most frequent in South Asians (31.8%), followed by East Asians (28.0%) and Europeans (15.0%), leading to a higher proportion of poor metabolizers in South Asians. The *3 allele was rare but slightly higher in South Asians (5.7%). The *17 gain-of-function allele was most common in Europeans (22.0%), moderate in South Asians (14.0%), and rare in East Asians (2.0%). Normal metabolizers predominated in Europeans...
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