Posted by admin on Nov 30, 2021 in |
In spite of huge investigations, the pathology of Alzheimer’ sdisease (AD) is uncertain. Vessels dysfunction is a critical mark of AD. Research has stated like beta-amyloid (Aβ) results augmented vascular development in brains, a pathway observed in AD patients. In AD, cerebral endothelium releases pro substances for β-amyloid plaque and neurotoxins that kill cortical neurons. Vascular vulnerable features and neural, vascular dysfunction related through hypo or hypertension, hypercholesterolemia, diabetes mellitus, smoking, oxidative stress, and iron overload have been invented to play essential parts in the pathogenesis of stroke and AD. Antiangiogenic agents and small molecule kinase blockers are being examined and approved for anticancer therapy and showed normal blood vessel growth in the affected areas. Endothelial cells (EC) are triggered by the angiogenesis of cerebral ischemia and hypoxia. Outcomes of epidemiological research show that chronic administration of NSAIDs, statins, H2 antihistamines, or calcium-channel blocker sappearstoavert AD. This is mainly due to the ability of the drugs to prevent angiogenesis. Many previous reviews on pathogenesis on AD have explained neuronal...
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Posted by admin on Nov 30, 2021 in |
Rabies is a fatal viral disease which is caused by negative sense, single-stranded RNA virus belonging to Rhabdoviridae family of genus Lyssavirus, Rabies Virus (RABV). It is transmitted by the bite of a rabid animal and directly affects the CNS of the host (neurotropic). Sources of human rabies deaths are dogs (99%), cats, bats, etc. Rabies holds the seventh position in the world for causing the most severe infections that result in the death of an organism. India lies on the top in the number of affected individuals with rabies. Our aim in this study is to analyze the interactions between the RABV and its human host and to develop novel therapeutic strategies to combat this deadly disease. Further analysis of the predicted protein-protein interactions can help us design strategies against clinical manifestations of the disease in humans. An in-silico approach has been adopted because computational approaches play a vital role in providing an important tool in further investigation of host-pathogen...
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Posted by admin on Nov 30, 2021 in |
Beta-blockers are widely used in combination with Ivabradine in people with coronary heart disease (CHD), one of the leading causes of death. Thus, monitoring of these drugs is important because it is accessible to manage heart failure amongst people with CHD. In addition, its quality control is fundamental to provide quality medicines. Method of analysis can be the first step in the rational use of pharmaceuticals. In this context, a detailed study of literature and official compendia for the pharmaceutical quality control of Ivabradine and Metoprolol were done. Among the analytical methods in the evaluation of Ivabradine and Metoprolol, HPLC is predominantly followed by HPTLC and UV. It was found that in the literature that analysis of Ivabradine and Metoprolol-based pharmaceutical products are more common than analysis of Ivabradine and Metoprolol in biological matrices. Pharmaceutical analyses have an impact on analytical decisions as well as effective and reliable results. The method must be suitable for the intended investigation. Although, there is a lack of Analytical Methods for estimation of...
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Posted by admin on Nov 30, 2021 in |
The present study evaluated the antimicrobial and cytotoxic activities of chloroform, hexane methanol, and petroleum ether extracts of Plumbago zeylanica (PEC, PEH, PEM, and PEP). Antimicrobial activities of the extracts were evaluated against gram-positive bacteria (Staphylococcus aureus-ATCC 6538 and Bacillus cereus-NCIM 2155) and gram-negative bacteria (E. coli-ATCC 8739) along with cytotoxicity activity against cervix carcinoma cell lines (HeLa and SiHa cell lines). Out of the four extracts tested, PEM and PEC extracts showed significantly high value of zone of inhibition against tested bacteria. PEM showed zone of inhibition of 10 mm, 13mm and 8 mm in ATCC 6538, NCIM 2155 and ATCC 8739, respectively. On the other hand, chloroform extract showed a zone of inhibition of 8mm, 10mm and 7 mm in ATCC 6538, NCIM 2155 and ATCC 8739, respectively. The four extracts PEC, PEH, PEM, and PEP showed a significant cytotoxicity activity with IC50 concentration at 238.93ug/ml, 694.44ug/ml, 580.40ug/ml and 593.04ug/ml against the HeLa Cell line and 142.71ug/ml, 547.16ug/ml, 448.04ug/ml and 424.27ug/ml respectively against the SiHa Cell line...
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Posted by admin on Nov 30, 2021 in |
Cryptococcus neoformans and Candida albicans are the major causative agents for infections, cryptococcosis, and candidiasis in human. Anti-fungal drugs for the treatment of fungal infections may show less effective activity on the pathogens due to the poor absorption or inadequate drug distribution, and some pathogens may evolve anti-fungal drug resistance. The development of anti-fungal drugs from natural sources with potential activity is of main global concern. Some filamentous fungi themselves can secrete anti-fungal compounds, which show effective antagonism against numerous pathogens. One such fungus, Aspergillus giganteus is known to produce anti-fungal proteins (AFP). In this study, the AFP is purified and its antagonistic activity was determined by analyzing its pathogenic membrane disruption ability. Extracellular pH and the lipid level in the AFP treated pathogenic cell membrane were also evaluated. The results have proved the membrane damage and disintegration of the pathogenic cell membrane by AFP and were observed in a dose-time-dependent manner. The fluctuation in the extracellular pH was monitored in the treated pathogens. This study has identified a...
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