Posted by admin on Nov 30, 2021 in |
In present study, Himalayan weed Sonchus oleraceus extracts are screened for the occurrence of bioactive compounds, anti-microbial activity, and its molecular characterization using RAPD. The agar well diffusion method was used to detect anti-microbial activity of aqueous, methanol, chloroform and ethyl acetate extracts of S. oleraceus. The anti-microbial activity of different extract was checked at different concentrations i.e. at 150mg/ml, 200mg/ml, 250mg/ml and 300mg/ml against five bacteria namely Klebsiella pneumoniae, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Salmonella typhi. The anti-microbial activity of the different extracts of S. oleraceus was associated with the concentration. In phytochemical screening alkaloids, flavonoids, protein, carbohydrates, tannins, phenol, terpenoids and saponins were confirm in different extracts. The primers OPA-1, OPA-9, OPA-16, TC-1 and TC-3 were used in molecular characterization using RAPD against 14 samples collected from five districts namely Kangra, Una, Bilaspur, Hamirpur and Solan of Himachal Pradesh. Cluster analysis revealed that isolates H1 and H3, H2 and S2, U1 and U2 were showing maximum homology. In comparison to all the isolates, isolate K1...
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Posted by admin on Nov 30, 2021 in |
The goal of the research was to explain as a cognition enhancer the traditional claim of Madhuca longifolia (Sapotaceae) leaves. Dementia was activated by intracerebrovascular colchicine administration for 28 days. Madhuca longifolia leaves ethanolic extract was evaluated in mice for cognition-enhancing activity in colchicine-induced dementia using elevated plus maze (EPM), actophotometer, and biochemical estimation of brain AChE and MDA levels. Piracetam (200 mg/kg p.o.) was used as standard medicine. Transfer latency (TL) was observed in the colchicine-induced mice using EPM, showing a significant increase (p<0.01) in TL compared to the regular control group. No major effect was found on the locomotor activity of the mice. In colchicine-induced mice, a significant increase (p<0.01) in AChE and a decrease in MDA levels relative to normal control were observed. These results formed the standard argument, and Madhuca longifolia can therefore be a memory enhancer because of the presence of...
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Posted by admin on Dec 31, 2020 in |
Ruthenium(II) complexes of the type [Ru(bpy) 2(L)](PF6)2, where L= 2-{4,5-bis[(E)-2-phenylethenyl]-1H-imidazol-2-yl}-1H-benzimidazole (1), 2-{4,5-bis[(E)-2-(4-chlorophenyl)ethenyl]-1H-imidazol-2-yl}-1H-benzimi-dazole (2), 2-{4,5-bis [(E)-2-(4-fluorophenyl) ethenyl]-1H-imidazol-2-yl} -1H-benzimidazole (3), 2-{4 ,5-bis[(E)-2- (4-methylphenyl) ethenyl] -1H-imidazol-2-yl }-1H-benzimidazole (4), 2-{4, 5-bis[(E)-2-(4-methoxyphenyl) ethenyl]-1H-imidazol-2-yl}-1H-benzimidazole (5) have been synthesized and characterized by elemental analyses and spectral (IR, UV-vis, NMR, ESI-MS) techniques. The redox behavior of the complexes has been studied by cyclic and differential pulse voltammetry. In acetonitrile solution, all the complexes exhibit characteristic metal to ligand charge transfer (MLCT) absorptions and ligand-based transitions. The complexes showed efficient DNA cleavage activity in the presence of light at the wavelength of 480 nm. The complexes are also able to cleave supercoiled pUC19 plasmid DNA via guanine base oxidation in a concentration-dependent manner. In-vitro cytotoxic activity of the complexes shows that complex 1 has better anticancer activity against MCF7 human breast cancer cells with an IC50 value of 7.9 µM. The antimicrobial activities of the ligand and their metal complexes were screened by agar diffusion method and found that the metal complexes have higher antimicrobial activity than the...
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Posted by admin on Nov 30, 2020 in |
Sickle cell anemia is a major life-threatening hemoglobin disorder in the world. This hemoglobin disorder is an autosomal recessive type, therefore considered as a major health problem in the world. The pain symptoms and complications of sickle cell anemia can be life-threatening many times. Management of sickle cell anemia demands drugs to be safe, well-tolerated, lesser side effects, and effective in improving the quality of life in patients. The alternative system of medicine can be the fruitful therapeutic option in conditions where the drug has to be consumed for life long. The T-AYU-HM Premium is a novel traditional Ayurvedic medicine designed on such a purpose to reduce clinical complications and enhance the quality of life in sickle cell anemia patients. The study conducted was a single dose oral toxicity study of T-AYU-HM Premium to assess its safety profile at a high dose. The study was conducted in compliance with the OECD principles of GLP (1998). The study was conducted according to OECD for testing of chemicals, N-425 acute oral...
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Posted by admin on Nov 30, 2020 in |
The inhibition of the protein cyclooxygenase (COX) is a well-known mechanism for achieving analgesia. Selective inhibitors of cyclooxygenase-2 (COX-2) provide excellent analgesia but can have side-effects. In this study, we chose ibuprofen, a non-selective COX inhibitor that has been safely used for a long time, to develop a novel, selective COX-2 inhibitor. The 3-dimensional structure of the drug target, COX-2, was obtained from the RCSB PDB online database and was input in SeeSAR. Three hundred and thirty alterations were made to ibuprofen molecule. Initial docking was performed in SeeSAR and CLC Drug Discovery Workbench to get a docking score. The 216 ligands that bound to COX-2 with the best binding score were then docked to cyclooxygenase-1 protein (COX-1), and a score was generated based on the binding affinity. Twenty-six of these molecules that didn’t bind to COX-1 were chosen as the selective inhibitors of COX-2 and these were tested for drug-likeness properties using the DruLiTo software. Twenty-two compounds with good drug-likeness properties were subjected to ADMET verification. 5 compounds...
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