Posted by admin on Mar 1, 2013 in |
Background: Black tea is rich in polyphenols which are antioxidants. The most abundant polyphenolic compounds are flavonols and catechins. Black tea antioxidants showed protective actions against chronic diseases like cardiovascular diseases due to their antioxidant action. Objective: The present study was conducted to evaluate the cardioprotective effects of Camellia sinensis on isoproterenol induced myocardial infarction in Wistar albino rats. Myocardial infarction (MI) is a condition in which a part of the heart muscle suddenly loses its blood supply, so the myocardium gets destructed and becomes ischemic. Camellia sinensis is a species of plant from which leaves and leaf buds are taken to produce tea. Myocardial infarction was induced by subcutaneous administration of Isoproterenol. Isoproterenol shows positive inotropic and chronotropic effects on heart. Oxidative products of drug cause cytotoxicity and myocardial damage. Methods: Wistar albino rats were divided into 8 groups, each group containing 8 rats. The following treatment was given for 21 days. Group 1: NS 1mg/kg (orally). Group 2: NS+ISO (85 mg/kg, s.c.). Group 3: BTE 1 (4mg/kg,...
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Posted by admin on Mar 1, 2013 in |
Endophytic fungi residing inside the medicinal plants are of gaining importance for their bioactive compounds. This present study is undertaken to isolate and identify the potential endophytic fungi from Moringa oleifera, a traditional medicinal plant. A total of 24 segments each 12 from leaf and stem were collected, surface sterilized and was inoculated on to Sabouraud Dextrose Agar (SDA) plates. Based on the macroscopic & microscopic features the fungal isolates were identified as Alternaria spp., Aspergillus spp. Bipolaris spp., Exosphiala spp., Nigrospora spp., and Penicillium spp. Many unidentified sterile mycelia forms were also found which were grouped under the class mycelial sterilia. The Colonization Frequency (CF) and Endophytic Infection Rate (EIR) were observed as 91.66% and 45.83% respectively. The results of this study suggest that traditional medicinal plants are a rich and reliable source of novel endophytic fungi. Further studies are required with regard to the screening of these endophytic fungi for the production of novel bioactive compounds which are medically important in the treatment of...
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Posted by admin on Mar 1, 2012 in |
Comparative study of free radical scavenging activity and phenolic acid content of Bulgarian endemic Alchemilla jumrukczalica and the commercial herbal – Alchemilla vulgaris complex was carried.The methanol extracts of studied samples showed significant antiradical activity. The concentration of the extracts needed for 50% inhibition of DPPH was found to be 12,09 µg/ml and 19,62 µg/ml for A. jumrukczalica and A. vulgaris complex, respectively.Ten free and seventeen bonded phenolic acids were identified and quantified by performed of gas chromatography mass-spectrometry (GC-MS). Gentisic, protocatechuic, salicylicand trans-cinnamic acids were represented in the greatest quantityamong the identified phenolic acids. This is the first detailed analysis on qualitative and quantitative composition of phenolic acids inAlchemilla...
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Posted by admin on Mar 1, 2012 in |
A simple, precise and stability-indicating HPLC method was developed and validated for the simultaneous determination of anti-hypertensive drugs Atorvastatin Calcium, Olmesartan Medoxomil, Candesartan, diuretics Hydrochlorothiazide and Chlorthalidone. The separation was achieved on Cosmosil PAQ (Length 150 mm × Diameter 4.6 mm Particle size 5 μm) column with gradient flow. The mobile phase at a flow rate of 1.0 mL min−1 consisted of 0.05 M sodium dihydrogen phosphate buffer and acetonitrile (Gradient ratio). The UV detection was carried out at 220 nm. The method was successfully validated in accordance to ICH guidelines. Further, the validated method was applied for commercially available pharmaceutical dosage...
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Posted by admin on Mar 1, 2012 in |
The present study was carried out to illustrate the relative evaluation of phytochemical constituents and antimicrobial activity of leaves of Eclipta alba and Aphanamixis polystachya and bark of Premna integrifolia in two different solvents. A preliminary phytochemical analysis was done and concluded that the presence of alkaloids, tannins, flavonoids and glycoside in both extracts of the above plants. The antimicrobial activity was tested using disc diffusion method against eight pathogenic bacteria using Kanamycin as antibiotic standards. The outcome showed that the antibacterial activity was effective in chloroform and ethyl acetate fractions of tested extracts. The MIC was determined in two different solvents of both extracts and observed that chloroform and ethyl acetate possess minimum inhibition in both extracts. From the current investigation, it can be concluded that Eclipta alba, Aphanamixis polystachya and Premna integrifolia crudeextracts have quite effective antimicrobial activity and potent phytochemical constituents....
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