Posted by admin on Mar 1, 2011 in |
A simple, sensitive, high throughput and specific HPLC method with UV detection was developed and validated to according to the FDA guideline for quantification of gemifloxacin in human plasma. The bioanalytical method consists of liquid-liquid extraction and quantification by UV detection. The analyte was separated isocratically on a Cyano column (250 X 4.6 mm, 5µ particle size) with a mobile phase consist of 1% v/v formic acid, acetonitrile and methanol in the ratio of 75:20:5 (v/v). The response to gemifloxacin was linear over range 0.025 to 2.000 μg/mL. The validation results demonstrate that the method had satisfactory precession and accuracy across the calibration range. Intra-and inter-day precession range from 0.676 to 1.222 and from 0.834 to 1.645% (RSD), respectively. The accuracy determine at three quality control samples was within 94.232 to 101.132%. There was no evidence of instability of the analyte in human plasma following the stability study. The method proved highly reproducible and sensitive and was successfully applied in bioequivalence study after oral dosing of gemifloxacin tablet in...
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Posted by admin on Mar 1, 2011 in |
The objective of this study was to review and explore the top fifteen currently herbal remedies used and the historically used herbal remedies in the treatment of psoriasis. Psoriasis is undoubtedly distressing, affected individuals are typically otherwise healthy and thus well suited to thoughtful outpatient care. Recent advances in our understanding of psoriasis have provided parallel advances in topical treatments. Recent advances in our understanding of psoriasis have provided parallel advances in topical treatments. Specifically herbals, there is limited scientific data regarding their benefits and interactions. About 75% of patients have mild-to-moderate psoriasis, amenable to topical treatment is lifetime controlling herbals remedies like Aloe, Cayenne, Chamomile, Dong Quai, Emu oil, Evening prime rose oil, Fish oil, Tea tree oil, Turmeric, Slippery elm, Wintergreen, Shark cartilage, Milk thistle, Glucosamine, Flexseed oil are needed. Herbal remedies for treatment psoriasis diseases to overcome the adverse effect, antagonistic effect and bioavailability of...
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Posted by admin on Mar 1, 2011 in |
Collecting blood from Lab animals is necessary for a wide variety of scientific studies, and there are a number of efficient methods available. It is important to remember that blood collection can stress the animals, and may have an impact on the outcome of research data. Potential adverse affects viz. Hypovolemic shock, anemia, stress, haemorrhage, bruising, thrombosis; infection at the site of needle entry, phlebitis, scarring, and nerve damage should be avoided. It is essential to be able to recognize the clinical signs of shock and to take appropriate action. The purpose of this article is to review the different methods of blood collection, their advantages, limitations, monitoring, restraints, possible adverse effects, their prevention and control in laboratory rodents....
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Posted by admin on Mar 1, 2011 in |
A phytotherapeutic approach to modern drug development can provide many invaluable drugs from traditional medicinal plants. Search for pure phytochemicals as drugs is time consuming and expensive. Numerous plants and polyherbal formulations are used for the treatment of liver diseases. However, in most of the severe cases, the treatments are not satisfactory. Although experimental evaluations were carried out on a good number of these plants and formulations, the studies were mostly incomplete and insufficient. The therapeutic values were tested against a few chemicals-induced subclinical levels of liver damages in rodents. Even common dietary antioxidants can provide such protection from liver damage caused by oxidative mechanisms of toxic chemicals. However, experiments have clearly shown that plants such as Picrorrhiza kurroa, Andrographis paniculata, Eclipta alba, Silibum marianum, Phyllanthus maderaspatensis and Trichopus zeylanicus are sufficiently active against, at least, certain hepatotoxins. Screening plants for antihepatitis activities remains in its infancy. P.kurroa, E. alba, Glycyrrhiza glabra, A. paniculata and P. amarus are likely to be active against Hepatitis B virus. In the case...
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Posted by admin on Mar 1, 2011 in |
The word herb, as used in herbal medicine, is also known as botanical medicine or as Phytotherapy or Phytomedicine which means a plant or plant part is used to make medicine to assist in the healing process during illness and disease. So there are many herbal remedies individually or in combination have been recommended in various medicinal treatises for the cure of different diseases. It contains various phytoconstituents belonging to alkaloids, glycosides, flavonoids, fixed oils, and carbohydrates. The roots of Pongamia pinnata are good for cleaning foul ulcers, cleaning teeth, strengthening gums and gonorrhoea. The root paste is used for local application in scrofulous enlargement. The fresh bark of Pongamia pinnata is sweet and mucilaginous to taste, soon become bitter and acrid. It is antihelmintic and useful in beri-beri, ophthalmology, dermatopathy, vaginopathy, and ulcers. Leaves of Pongamia pinnata are digestive, laxative, antihelmintic and are good for diarrhea, leprosy, dyspepsia and cough. Flowers are useful to quench dipsia in diabetes and for alleviating vata and kapha. The seeds are antihelmintic,...
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