Posted by admin on Feb 28, 2021 in |
Dabigatran mesylate is a novel oral anticoagulant that works by blocking the clotting protein thrombin. The present work describes the separation and quantification of potential impurities in a single HPLC method, which are generated from raw materials to API synthesis. This method is capable of separating and quantifying the fourteen impurities, produced from the intermediate stages to final drug substance stage of dabigatran within 40 min of run time. Column: Poroshell 120, EC C-18, 150 mm × 4.6 mm, 2.7 µ; Buffer: 2.04 g of Potassium dihydrogen phosphate in a beaker, add 1500 mL of water and dissolve, to this add 1.5 ml Triethylamine (TEA) and 1 ml of Phosphoric acid; Diluent: water and acetonitrile in the ratio 20:80% v/v; The flow rate: 0.7 mL/min; Column temperature: 40 °C. Wavelength: 230 nm; The drug substance was subjected to stress studies such as hydrolysis, oxidation and thermal degradation and considerable degradation was observed in acidic hydrolysis and oxidative stress conditions. The formed degradation products were well-resolved from the dabigatran drug...
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Posted by admin on Feb 28, 2021 in |
Benzofuranones and nitrogen mustards have been reported as highly potential alkylating agents; with this evidence, the synthesis of some benzofuranones fused with nitrogen mustards was planned and was subjected to in-vitro cytotoxic studies. Substituted benzofuranones were synthesized by condensation of 2-hydroxy benzaldehydes and substituted 2-hydroxy acetophenones and further fused with nitrogen mustards gave high yields of target compounds 2 – (2 – (2 – (Bis (2 -chloroethyl) amino) ethoxy) benzylidene) benzofuran-3(2H)-one derivative. The derivatives synthesized had various halo substitutions such as chloro, bromo, fluro and methyl chloro derivatives. These synthesized compounds were characterized by FTIR, 1H NMR and LCMS spectral studies. Further, the synthesized compounds were subjected to 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay on human lung carcinoma cells, A-549 and breast cancer cells, MCF-7 for its in-vitro cytotoxic activity. All the synthesized compounds showed promising cytotoxic activity in which AN– O – 04, (Z) – 2 – (2 – (2 – (bis (2 -chloroethyl) amino) ethoxy) benzylidene) -5 – chloro – 6-methylbenzofuran-3(2H)-oneshowed minimum CTC-50 of 119.32 ± 8.98...
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Posted by admin on Feb 28, 2021 in |
The effect of constant lighting on the level of some hematological parameters and their daily dynamics was studied in male Wistar rats at the age of 6 months. It was found that staying in constant light for 3 weeks leads to a rise in hematocrit level, mainly due to an increase in the number of red blood cells, while a decrease in the oxygen-transporting properties of these cells is noted. An analysis of the diurnal dynamics of the studied parameters, it was found that the keeping of animals under constant illumination destroys the circadian rhythms of HGB, MCV, RDWc, PCT, MPV, PDWc, and MCHC. The destruction of the circadian rhythms found in control is most likely due to both a decrease in the level of melatonin in constant light and a violation of its own circadian rhythm in the absence of a pacemaker, which role is played by the alternation of light and...
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Posted by admin on Feb 29, 2020 in |
Biosynthesis of nanoparticles by biological means especially plant extract plays a major role in the field of nanotechnology. In the present study, the aqueous extract of lemon peel (Citrus limon) was used for the green synthesis of ZnO nanoparticles using 0.1 M Zn(NO3)2 as a precursor in alkaline condition using NaOH with vigorous stirring for 3 h. ZnO NPs obtained were characterized by UV-vis spectroscopy in which the surface Plasmon resonance was 310 nm, followed by XRD, FTIR that showed appropriate speaks for various chemical groups present including ZnO NPs and AFM results shows monodispersed in nature. Further, the antibacterial property of ZnO NPs synthesized by the green method was revalued by the well diffusion method. The zone of inhibition was found to be 9 mm for 100 µL and 7 mm for 100 µL for respective strains Klebsiella aerogene and Serratia marcescen. Significant antioxidant activity was exhibited by NPs through scavenging of 1, 1-Diphenyl-2-picrylhydrazyl (DPPH) free radicals. Thus, the use of aqueous peel extract as a capping agent...
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Posted by admin on Feb 29, 2020 in |
Background: Subnormal level of vitamin D are associated with the higher frequency of cancer and correlated with inferior prognosis in some cancers; no data exist for acute leukemia. Aim of the work: To clarify the relationship between serum vitamin D levels and acute leukemia in children. Materials and Methods: Vitamin D level was measured in sixty-six pediatric patients who are newly diagnosed with acute leukemia in Central Child Teaching Hospital during a period between March 5th to August 30th, 2017 and normal 50 children were considered as the control group. Information on the patients were obtained from the medical files of the patients. Patients were classified on a random base as active patients group how the received daily requirement of vitamin D during induction chemotherapy period and passive patients’ group who were treated with chemotherapy only. Vitamin D level was reassessed at the time of remission. Results: The serum vitamin D level in children with acute leukemia was below normal ranges; at the time of diagnosis, the mean of...
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