Posted by admin on Mar 31, 2018 in |
The MAP kinase phosphatases regulate the magnitude and kinetics of MAP kinase signalling within the cell and as a consequence cell cycle progression and division. Therefore, these enzymes have been implicated in the regulation of number of disease conditions. One member of this family, MKP-2, has been linked to cancers of the breast and prostate. However, in human cells correlating changes in MKP-2 expression and effects upon ERK signalling, the main substrate for MKP-2 has been difficult, due to lack of convincing cellular analysis. Therefore, we utilized a novel lentivirus shRNA MKP-2 construct (L.001) to examine the consequences of knockdown on the regulation of ERK MAP kinase signalling. Infection of LNCaP AI cells with lentivirus shRNA MKP-2 reduced endogenous MKP-2 mRNA by approximately 80% in LNCaP cells. Immunofluorescent GFP labelling revealed strong cellular expression of MKP-2, approximately 70% of the cells were infected. MKP-2 knockdown reduced adenoviral induced hMKP-2 expression by approximately 50%. Both EGF and FCS induced strong but transient activation of ERK phosphorylation in LNCaP (AI), EGF...
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Posted by admin on Mar 31, 2018 in |
Medicinal applications of metals and its compound were reported centuries ago but it gained huge appreciation after the discovery of anticancer activity of metal based drug cisplatin. After cisplatin discovery many medicinal synthetic chemist moved into the field of organometallics for combating diseases and investigating the excellent pharmaceutical potential of metal complexes, giving birth to modern medicinal organometallic chemistry. Derivatization of the already existing drugs is the emerging field of pharmaceutical and synthetic medicinal chemistry. Working on metal complexes of already approved drugs can substantially reduce the time and money spent in clinical development. Organotin(IV), Cu(II), Zn(II), Fe(III) and Sb(III) complexes of Ribavirin were synthesized keeping the same in mind. These complexes were characterized by elemental analysis, IR, 1H, and 13C NMR spectroscopic studies. The synthesized complexes and the parent drug were screened for in-vitro biological activities. Interestingly some of the synthesized metal complexes of ribavirin have shown impressive antibacterial and antifungal on the other hand ribavirin was found to be...
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Posted by admin on Apr 27, 2017 in |
Today’s generic drugs manufacturing industry need robust analytical methods for analysis of generic drug products to provide best quality pharmaceutical formulation. Entacapone, Levodopa and Carbidopa tablets is such a product which is used for the treatment of Parkinson’s disease. This paper presents a robust HPLC method that has been developed for estimation of % drug release of Entacapone in this multi component drug formulation. HPLC column used for separation was Cosmosil 5PE-MS 150mm x 4.6 mm with 5 micron particle size. Combination of phosphate buffer pH 2.5, acetonitrile and methanol was used for mobile phase in isocratic mode with UV detection at 280 nm. The method was validated to ensure suitability of the method for quantitative determination of % drug release of Entacapone in presence of Levodopa and Carbidopa in this triple drug combination pharmaceutical formulation. Proposed method was found to be specific, precise, robust, accurate and linear in range 0.066 to 0.33 mg/mL of...
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Posted by admin on Apr 12, 2017 in |
Staphylococcus aureus biofilm has been known as one inducing factor for the bacteria’s resistance to various antibiotics. One strategy which may increase the efficacy of the antibiotic is by combining the antibiotic therapy with antiinfective from natural resources. This research was evaluating the potency of three known antiinfective essential oil derived from leaves of Piper betle L., Ocimum basilicum L. forma citratum Back and Cymbopogon citratus L., in a combination with antibiotics i.e. chloramphenicol, streptomycin, and erythromicin towards S. aureus ATCC 29213. The essential oils were obtained by steam-hydrodistillation of the fresh raw materials. Microdillution technique combined with colorimetric was used to determine the biofilm inhibition. Crystal violet was used for biofilm staining of which the reading was performed on a microplate reader. Fractional Inhibitory Concentration Index (FICI) values was evaluated based on the comparison of % inhibitory obtained from the essential oils and the antibiotics in a single and in a combination. The essential oils alone has the PMIC50 (planktonic) values as follows, 0.2% (P. betel), 0,3% (C....
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Posted by admin on Mar 31, 2017 in |
Resolvins and lipoxins endue a novel way of approaching aspirin mechanism of action by developing models. Recent advances in research of aspirin from starting point Willow bark (Salix alba) to resolvins and lipoxins along with their repurposing network. Therefore, aspirin have a big impact on most interesting areas of research. Aspirin and there interaction with several targets molecules (Magic shotguns) give the option in the uses of various disorder but there MOA based uses in all cases not limited to COX rather than newer ones like resolvins, lipoxins, protectins and maresins, In particular, resolvin and lipoxins will play a central part while research aspirin and their rescheduling in different human diseases that’s are better emphasize by novel MOA of...
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