Posted by admin on May 31, 2016 in |
Amino acid chelated complexes were synthesized and characterized to determine their purity. The purity of the complexes was found to be ranging from 42-75%. The HPLC method for determination of free amino acid content in the complexes was developed and validated and found to be linear, precise and accurate within concentration range of 0.157 mg/mL to 20.14 mg/mL. The method was used to estimate the purity of the amino acid complexes. The changes in the carboxylate and amine group stretches in FT-IR indicated the association of metal ion with the amino acid. The initial studies described above demonstrated that the chelated complexes can produce sufficient osmotic pressure to be used as ergogenic...
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Posted by admin on May 31, 2016 in |
Bioanalytical method development is the process of creating a procedure to enable a compound of interest to be identified and quantified in a biological matrix. A compound can often be measured by several methods and the choice of analytical method involves many considerations. Analysis of drugs and their metabolites in a biological matrix is carried out using different extraction techniques like liquid-liquid extraction, solid phase extraction (SPE) and protein precipitation from these extraction methods samples are spiked with calibration (reference) standards and using quality control (QC) samples. These methods and choice of analytical method describes the process of method development and includes sampling, sample preparation, separation, detection and evaluation of the results. The developed process is then validated. These bioanalytical validations play a significant role in evaluation and interpretation of bioavailability, bioequivalence, pharmacokinetic, and toxicokinetic studies. In which different parameters like accuracy, precision, selectivity, sensitivity, reproducibility, and stability are...
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Posted by admin on May 31, 2016 in |
Introduction: Epidural anesthesia is the most commonly used technique for providing not only peri-operative surgical anesthesia but post-operative analgesia in lower abdominal and limb surgeries. Our study aimed to assess the comparison between ropivacaine(Group A) and combination of ropivacaine with fentanyl (Group B) in terms of their analgesic effect, hemodynamic stability, side effects and requirement of rescue drug in post operative patient of total knee replacement surgery. Materials and methods: After surgery, Pulse rate, systolic and diastolic blood pressure, Sp02, visual analogue score and requirement of other analgesics was studied at every 30 minutes for 2 hrs, then hourly up to 12 hrs and every 2 hourly thereafter up to 24 hrs in group A and Group B. Patients were also observed for side effects. Statistically significant when the p <0.05 and highly significant if p<0.001. Results: In our study, there was no significant difference amongst the groups in respect to age, sex distribution, height and ASA physical status. There was no statistically significant difference in pulse rate, blood...
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Posted by admin on May 31, 2016 in |
Medicinal Plants are endowed with phytochemicals that are vital to counter various metabolic disorders like Oxidative damage in cells causing various degenerative diseases. Hence, the present study deals with the assessment of antioxidant activity and phytochemical screening of the aqueous extract of Jasminum officinale leaves. Antioxidant potential was evaluated in vitro using free radical scavenging assays for DPPH, NO, superoxide and ABTS radicals in addition to Reducing Power assessment. The total phenolic, flavonoid and flavonol contents of the extract were also assessed using standard procedures. The phytochemical analysis of the extract revealed the presence of tannins, flavonoids, terpenoids, alkaloids, steroids and its glycosides in addition to saponins and coumarins. A positive correlation between the inhibition of free radicals and concentration of the extract suggests significant antioxidant potential of the extract. The IC50 values for DPPH, NO, superoxide and ABTS radicals were 41.16, 30.29, 20.19, and 29.48 µg/ml respectively as compared to the standard, ascorbic acid, having 42.79, 36.74, 38.22, and 45.57 µg/ml, for the same radicals. It is interesting...
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Posted by admin on May 31, 2016 in |
The use of nanoparticles as drug delivery and targeting agents has led to immense progress in the field of biomedicine and diagnostics. Likewise gold nanoparticles can be conjugated with various molecules, for instance, to plasmid DNA for gene therapy, to various drugs for antibacterial and anticancer properties, to several antibiotics and peptides for immunological and diagnostic purposes. The review encompasses various fields in which the gold nanoparticle conjugates have emerged as a promising tool for effective therapeutics and diagnosis. The article addresses a brief knowledge about the subject available to readers about various gold nanoparticle conjugates that have been researched. Although more work is needed to establish the toxicity concerns yet these particles have proved to be a promising therapy for the diagnosis and treatment of various diseases, especially cancer. With the cancer cells becoming resistant to anti-cancer drugs, the use of gold nanoparticles for the enhanced delivery and increased action of anti-cancer drugs has paved a new way to battling against...
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