Posted by admin on Jun 30, 2015 in |
We intended desired ointment developed for bacterial infection. So Levofloxacin is a fluoroquinolones of antibacterial drug effective in the treatment of bacterial conjunctivitis, sinus, kidney, bladder or prostate Here the main objective of the present work is besides delivering drugs to the body, A drug delivery system to improve patient compliance and dispersible are no exception. So we have selected the topical drug delivery system by using a formulation of ointment. The Levofloxacin ointment is prepared by the two ointment bases, namely Oleaginous and Emulsion bases. To assess the efficiency of Levofloxacin ointment by physical appearance, viscosity, PH and spreadibility, Rheological properties and micro biological studies. The Oleaginous base prepared by using white bees wax and petrolatum and emulsion base having the methyl paraben, propyl paraben, sodium lauryl sulfate, propylene glycol, stearyl alcohol white, white petrolatum, purified water. The % of drug content of Levofloxacin was found to be 99.53. The PH was found to be 5.1 % colour was found to be formulation A (Oleaginous) is light yellowish...
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Posted by admin on Jun 30, 2015 in |
There are millions of micro-organisms around us, amongst 20-25% are non pathogenic. Pathogenic microbes present in the environment around us are responsible for various health related problems. Reducing microbial load to non-pathogenic levels may be the purpose of traditional fumigation techniques like Dhoopana, Homa, and Havana etc. This is also evident from Ayurvedic literature wherein fumigation is indicated for sterilisation of operation theatres as well as labour theatres and also in wound management. Taking all these into consideration, in present study an attempt is made to estimate anti microbial effect of Dhoopana of an indigenous fumigation formulation ‘DHUP’. Two rooms of same size which are in routine usage in almost similar fashion were selected for the study. One room served as control without any intervention. In the second room Dhoopana was performed once daily for two days. In both the rooms agar gel plates were exposed at selected places and efficacy of DHUP Dhoopana was noted at selected intervals. The microbial load was estimated and subjected to statistical analysis....
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Posted by admin on Jun 30, 2015 in |
A simple, sensitive, precise, accurate and cost effective reverse phase high performance liquid chromatographic method for the simultaneous estimation of bupropion HCl and naltrexone HCl from a single formulation was developed and validated in accordance to ICH guidelines. The two drugs were eluted under isocratic mode using a 250 x 4.6 mm i.d, 0.45 µm Phenomenex Chromosil C18 column. The mobile phase was composed of a mixture of methanol, acetonitrile and water in the ratio of 60:20:20 v/v (pH adjusted to 4.8) at a flow rate of 1.0mL/min. The limit of detection and limit of quantification were found to be 0.5μg/mL and 1.7μg/mL, for both the drugs. The label claim of the combined dosage form was in good agreement with the experimental assay amounts; 99.5% and 99.7% for bupropion HCl and naltrexone HCl, respectively. This method can be successfully applied for the simultaneous estimation of bupropion HCl and naltrexone HCl combination for routine quality control...
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Posted by admin on Jun 30, 2015 in |
The term Hydrotherapy refers to a process which uses water at any temperature or forms to relieve pain and treat illness. This review describes about various conditions of water treatments. Hydrotherapy may help treat various conditions, including arthritis, stomach issues, sleep disorders, stress and depression. The theory behind hydrotherapy is that water has healing properties that can relieve various ailments and conditions. In its different forms—ice, liquid, and steam—water is a versatile treatment method. Water cure therapies includes enema therapy, gel therapy etc, which includes colonic hydrotropy, sitz bath, hydro-massage, wraps and compress methods. Recently we have a number of hydrotherapy treatments available which includes personal infrared sauna rooms and peat bath rooms. These are useful in pregnancy, heart or lung conditions, diabetes, neuropathy, high fever, acute headache, surgical implants, seizure disorders etc. Cryotherapy, cold water immersion or ice bath is a new form of hydrotherapy used by physical therapists, sports medicine facilities and rehab...
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Posted by admin on Jun 30, 2015 in |
Peroxidase enzyme plays an important role in pharmaceutical, Industrial and various research laboratories. This enzyme can be purified by various different method. Our research trend was to purify the enzyme in a convenient and easy way. For this purpose a new affinity column was prepare by immobilizing PABA to N-HydroxySuccinamaideagarose gel. The presence of peroxidase was investigated on various Bangladeshi vegetables, such as, daikon, cabbage, cauliflower, tomato and sweet potato. Based on a previous screening experiment, daikon contains the highest amount of peroxidase. The juice of daikon was first extracted by tincture press and subjected to ammonium sulphate precipitation. The precipitate was dissolved in 25 mM phosphate buffer, pH7.4 containing 500 mMNaCl, 1 mM MnCl2 and 1 mM CaCl2. The crude extract thus obtained was subjected to an inhibitor affinity chromatography (IAC) method. Here, we have taken advantage of the affinity of peroxidase toward p-aminobenzoic acid hydrazide (PABAH) and subsequent recovery of the peroxidase by a known destabilizing agent ascorbic acid. The partially purified sample was applied to the...
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