Posted by admin on Jul 2, 2014 in |
Meningitis is a condition whereby the protective membranes covering the central nervous system (or meninges) become inflamed. Infections of the central nervous system are still considered to be among the most debilitating diseases in the 21st century. The mortality from this infection ranges from 15% in industrialized to 40% in developing countries. Streptococcus Pneumoniae infections, including pneumococcal meningitis, are therefore likely to remain an important health issue. Pneumococcal meningitis in human beings is associated with long-term sequelae including sensory-motor deficits, seizures, and impairments of learning and memory. Neurological sequelae occur in up to half of the survivors of pneumococcal meningitis. Meningitis is manifested as severe headache, occurring in almost 90% of cases of bacterial meningitis, followed by nuchal rigidity. Meningitis is a potentially serious condition due to the proximity of the inflammation to the brain and spinal cord. The potential for serious neurological damage or even death causes meningitis to need immediate medical attention and...
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Posted by admin on Jul 2, 2014 in |
Saliva plays the most important role in the maintenance and preservation of the health of our oral cavity. In diabetes mellitus functions of salivary glands are impaired and as a consequence xerostomia develops. Hoposalivation leads to various oral mucosa alterations, ulcerations, taste alterations, burning mouth, dental decay, halitosis and various infections. All these alterations can compromise patient’s quality of life. Saliva can also be used as a diagnostic tool as well as marker for monitoring diabetes...
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Posted by admin on Jul 1, 2014 in |
A Cyclodextrin glycosyltransferase (CGTase) producer was isolated from soil obtained from rice fields using alkaline Horikoshi II medium. The alkaliphile was characterized microscopically, biochemically and confirmed by 16S rRNA analysis as Bacillus oshimensis. CGTase production is dependent on the strain, medium composition and culture conditions; hence media optimization studies were carried out in shake flask using the statistical method. Tapioca starch was used as carbon source and a combination of yeast extract and peptone were used as nitrogen source in the media, along with MgSO4.7H2O, K2HPO4 and Na2CO3. A 33 factorial design using Taguchi method has been chosen to elucidate the combined effect of these variables. The parameters were optimized by changing three independent variables, with fixed concentration of mineral sources. The optimal media composition for CGTase production was found to be comprised of Tapioca starch 1%; peptone 0.5%; yeast extract 0.5% and 0.14% Magnesium Sulphate, 0.2% ammonium dihydrogen phosphate according to the factorial designing of the experiment and was used for further studies. The CGTase activity was found...
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Posted by admin on Jul 1, 2014 in |
The formation of free hydroxyl radical (˙OH) in oxygen transfers at GCE and the reaction between the electrochemically produced ˙OH and salicylic acid (SA) is studied by cyclic voltammetry method. Experiments showed that ˙OH is formed in the potential range of 1.0-1.4V corresponding to the anodic discharge of H2O at the GCE. The interaction of Cu2+, Hg2+, Cd2+, Mn2+ ions on the hydroxylation reaction is studied. The interaction of these metal ions is explained by using derived parameters such as surface coverage, formal potential, transfer co-efficient number and rate constant. The diffusion coefficient values for electroactive species on GCE is determined by using chronoamperomety and chronocoulometry techniques. It shows that Mn-SA has higher diffusion coefficient value as compared to the other systems. Multisweep cycle experiment on GCE surface provided mechanism for the reaction of hydroxylation on salicylic acid. This experiment suggests that metal ions first undergo Fenton-type hydroxylation reaction on salicylic acid and then undergo complexation reaction with 2, 5-dihydroxybenzoic...
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Posted by admin on Jul 1, 2014 in |
Objective: The present study was aimed to evaluate the antidiabetic activity of 1-(4-(Dimethylamino) benzylidene)-5-(2-oxoindolin-3-ylidene) thiocarbohydrazone i.e. a novel Isatin derivative diabetic rats. Methods: The antidiabetic activity of Isatin derivative was evaluated against alloxaninduced diabetes in rats. Diabetes in rats was induced by administration of alloxan monohydrate (120mg/kg, b.w, p.o) as a single dose. The diabetic rats were treated with single dose of test compound at a dose of 50 and 100mg/kg. Blood samples were collected at different intervals and analyzed for fasting glucose levels, total cholesterol (TC), triglycerides (TG) and High density cholesterol (HDL-C) levels using respective kits. Results: Treatment with test compound with a single dose of 50 and 100mg/kg to diabetic rats showed a significant dose dependant (p<0.01) reduction of fasting blood glucose levels along with mild change of lipid profiles (TC, TG, HDL-C) indicating the antidiabetic activity of test compound. Conclusion: The study results confirmed the antidiabetic activity of 1-(4-(Dimethylamino)benzylidene)-5-(2-oxoindolin-3-ylidene) thiocarbohydrazone in...
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