Posted by admin on Sep 1, 2014 in |
Genetic relationship between ten wild cultivars of C. roseus L. from four different regions of Rajasthan (Jaipur, Bikaner, Kota, and Jhalawar) with the marked difference in climatic condition was investigated using RAPD markers. Catharanthus roseus L. is a storehouse of several secondary metabolites such as vindoline, catharanthine, vinblastine, and vincristine, etc., thus making it an important source of drugs. A number of varieties of C. roseus L. occurs in wild and are cultivated in India. However, an evolutionary study has been limited. Using 24 decamer primers, we obtained a total of 221 amplicons with an average of 9.20 bands per primer. Out of 221 bands, 187 were found to be polymorphic, and the level of polymorphism was 84.61 percent. The average number of polymorphic bands per primer was 7.79. The average genetic similarity coefficient observed was 0.51 ± 0.186. Phylogenetic tree constructed using the neighbor-joining method of cluster analysis separated all the 10 samples of the C. roseus into two clusters. RAPD analysis thus was effective in differentiating the...
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Posted by admin on Sep 1, 2014 in |
In this study, the phytochemicals and their antimicrobial potential of flowers of Bauhinia blakeana of Fabaceae Family were evaluated. The selected microbes for the antimicrobial study were E. coli, Enterobacter sp., Proteus sp., and Klebsiella sp. Ethanolic extract was prepared by continuous hot percolation method using Soxhlet extractor, and Antimicrobial activity was tested by Agar well diffusion method using Streptomycin as reference. The phytochemicals present in Bauhinia blakeana flowers were screened by standard method. The phytochemical evaluation revealed the presence of Alkaloids, Flavonoids, Glycosides, Terpenoids, Anthocyanins, Phytosterols, Tannins, Carbohydrates, Saponins, and Phenols. Among the phytochemicals, the Alkaloids and Flavonoids were showed strong results in the phytochemical screening. The Antimicrobial investigation revealed the extract of the flower showing significant Antimicrobial potential against the selected microbes. It showed maximum mean zone of inhibition of 12mm against Klebsiella sp. and a minimum of 7mm against Enterobacter sp. Further research on this plant flowers is to be...
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Posted by admin on Sep 1, 2014 in |
Candida albicans has been the most widely premeditated pathogen in antifungal resistance because of their morbidity and mortality allied with infections in immunocompromised patients. A variety of mechanisms of resistance exist, with the most significant being those due to efflux pumps. The emergence of resistance during therapy in other settings appears uncommon but has been described in settings other than HIV-infected patients. We studied azole-resistant mechanism in 20 isolates of Candida albicans from HIV infected patients; these mechanisms include the presence of point mutations in the ERG11 gene and several genes encoding efflux pumps. Using Northern blot analyses, the expression patterns of these genes have been determined during logarithmic and stationary phases of cell growth. Point Mutations and overexpression of ERG11, and several genes encoding efflux pumps, as measured by quantitative real-time reverse transcriptase polymerase chain reaction in 20 clinical isolates of itraconazole resistant C. albicans strains. CDR1, CDR2, and MDR1 are expressed early on during logarithmic growth, CDR4 is expressed late during logarithmic growth, and CDR1 is preferentially...
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Posted by admin on Sep 1, 2014 in |
The investigation was aimed at evaluation of the mechanism of anticancer activity of the glycosides from Calotropis gigantea on breast cancer MCF-7 cells through analysis of modulation of gene expression. Cardiac glycosides were isolated from the leaves of C. gigantea, and the compounds were identified through LC-MS. Influence of the glycoside sample on the expression of p53 and Bcl-2 genes in MCF-7 cells was assessed by comparative RT-PCR of MCF-7 cells treated with the test sample, standard drug, and control followed by validation through colony formation assay. Presence of calactin, calotropin, asceplin, and cymarin along with 4 other unidentified compounds has been detected in the glycoside fraction isolated from the leaf sample of C. gigantea. Influence of the glycosides on the modulation of the expression of tumor suppressor gene p53 indicated a weak down-regulation of its expression, whereas on the modulation of Bcl-2 was found to be drastic with almost absolute inhibition of its expression. Results of the study suggest that cardiac glycosides cast a dual influence on MCF-7...
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Posted by admin on Sep 1, 2014 in |
Several formulations are marketed globally, claiming to be useful in diabetes & other associated diseases. In the present study, an attempt has been made to study the comparative efficiency of herbal formulations, for their hypoglycemic activity. The study was conducted on healthy rats randomly distributed streptozotocin (STZ) induced diabetic control & test rat groups. In diabetes-induced rats fed with herbomineral formulation 500 mg/kg & 1000 mg/kg. The hypoglycemic activity was studied by measuring the reduction in blood glucose levels & increased body weight at different time intervals. The present investigation was undertaken to evaluate the Antidiabetic activity of herbomineral formulation containing five different herbs & two minerals in streptozotocin (STZ 50 mg/kg ip single dose) induced diabetic rats. Treatment with formulation F1 (500 mg/kg) & F2 (1000 mg/kg) by oral administration for 4 weeks produced a significant reduction in blood glucose level & increase body weight in diabetic rats. The efficacy of plant extract was comparable with Glibenclamide & Insulin, a well known hypoglycemic drug. In STZ induced diabetic...
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