Posted by admin on Aug 31, 2015 in |
A simple, economical, specific, accurate, precise and validated Reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for simultaneous estimation of Phenylepherine hydrochloride (PPEH) and Cetirizine hydrochloride (CETH) in combine dosage form. The chromatographic separation was achieved on Princeton SPHER C18 column (250 mm x 4.6 mm id, 5 μ particle size) at ambient temperature using mobile phase buffer (0.1 M Ammonium dihydrogen phosphate pH 5.2 ± 0.05) : Acetonitrile (50:50% v/v) at flow rate 1.0 ml/min. Quantification was achieved with UV detector at 225 nm. Retention time of Phenylepherine hydrochloride and Cetirizine hydrochloride were found to be 2.19 ± 0.05 minute and 4.16 ± 0.05 minute respectively. Linearity was studied in the concentration range 5-30 μg/ml and 10-60 μg/ml for Cetirizine hydrochloride and Phenylepherine hydrochloride respectively with a correlation coefficient of 0.9998 and 0.9998 respectively. The proposed method was validated according to ICH guidelines with respect to linearity, accuracy, precision, robustness, LOD, and LOQ. The developed method with good separation, successfully applied for determination of PPEH...
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Posted by admin on Aug 31, 2015 in |
This paper presents the synthesis and physical-chemical characterization of a new terpyridine (tpy) ruthenium complex coordinated to the L-type calcium blocker channels verapamil (vera) obtained from commercial pill. Elemental analysis (%C N H), infrared (IR) and ultraviolet-visible (UV-vis.) spectroscopy, mass spectrometry (MS), and 13CNMR (nuclear magnetic resonance), and HPLC (high performance liquid chromatography) confirmed the pure and structure of the ligand verapamil and of the ruthenium complex structurally designed as [RuIICl2(tpy)(vera)] Cyclic voltammetry aided investigation of the electrochemical behavior of [RuIICl2(tpy)(vera)] based in the metal ion redox potential. Verapamil ligand and [RuIICl2(tpy)(vera)] induced vascular relaxation in endothelium-intact and endothelium-denuded aortic rings. Verapamil drug and [RuIICl2(tpy)(vera)] complex coupled to verapamil elicited similar pharmacological results. The calcium ion chelating effects (Kd) promoted for verapamil ligand and [RuIICl2(tpy)(vera)] complex were found based in the fluorescence technic. The smooth muscle cell images studied to [RuIICl2(tpy)(vera)] complex acting as a luminescent agent important as biological...
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Posted by admin on Aug 31, 2015 in |
This study was carried out to evaluate the effect of boiling on amino acids contents of the fruits of four species of the family Cucurbitaceae which were Cucurbita maxima D, Cucurbita pepo L, Cucurbita moschata and Lagenaria siceraria variety supreme court. In this study, fresh fruit of the four species were subjected to conventional processing technique which is boiling as well as drying. The dry and boiled fruits were evaluated for essential and nonessential amino acids content as well as total amino acids. Amino acids profile of boiled and dry fruits showed the presence of eight essential and nine non essential amino acids. The four species recorded a considerable amount of proline as follows: L. s. uncooked (370 mg/100gm), C. mo. uncooked (318 mg/100gm) L. s. cooked (258.8 mg/100gm), C. p. uncooked (232mg/100gm) and C. p. cooked (208mg/100gm). The highest total amount of amino acids of boiled fruits was recorded in Lagenaria siceraria (4977.6mg/100gm) followed by Cucurbita pepo (2870.1mg/100gm) where as the lowest was recorded in Cucurbita maxima (1427mg/100gm). ...
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Posted by admin on Sep 2, 2014 in |
Co-crystals consists of API and a stoichiometric amount of a pharmaceutically acceptable co-crystal former. Pharmaceutical Co-crystals are nonionic supramolecular complexes and can be used to address physical property issues such as solubility, stability, and bioavailability in pharmaceutical development without changing the chemical composition of the API. Cocrystal is a crystalline entity formed by two different or more molecular entities where the intermolecular interactions are weak forces like hydrogen bonding and π- π stacking. Super porous systems, biodegradable hydrogel systems. Co-crystallization alters the molecular interactions and composition of pharmaceutical materials and is considered a better alternative to optimize drug properties. Co-crystals offer a different pathway, where any API, regardless of acidic, basic, or ionizable groups, could potentially be co-crystallized. This aspect also helps complement existing methods by reintroducing molecules that had limited pharmaceutical profiles based on their nonionizable functional groups. The article gives a brief review of the co-crystallization, its difference from other states and its importance as an alternative over slat formation. The review also highlights the current FDA...
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Posted by admin on Sep 1, 2014 in |
Spirulina platensis, planktonic blue-green algae, has been used as a source of protein (60-70%) and vitamin supplement for more than 15 years without any unknown adverse events. Traditionally, Spirulina platensis has been used as an antioxidant agent. However, no pharmacological work has been done to evaluate the anti-anxiety activity. The present study was premeditated for the evaluation of the anti-anxiety activity of Spirulina platensis. Elevated plus maze (EPM) and light-dark models were used to evaluate the anti-anxiety potential of Spirulina platensis in Swiss albino mice. Albino mice were treated orally with diverse doses of the Spirulina platensis (i.e., 300 and 400 mg/kg). Diazepam (2 mg/kg, IP) was used as a standard drug. In conclusion, 400 mg/kg dose of Spirulina platensis showed significant anti-anxiety activity (P<0.05) while the control group was near to standard group. It concludes that Spirulina platensis could be useful as an anti-anxiety agent for...
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