Posted by admin on Aug 31, 2022 in |
Neuropathic pain (NP) is a chronic debilitating condition that affects all dimensions of health. Though pharmacological therapy is the main treatment of varied neuropathic pain conditions, not all neuropathic pain conditions respond well to drug therapies. Therefore, under-utilized non-pharmacological strategies were tried and implemented in chronic neuropathic pain subjects to provide adequate pain relief and restore functionality. This study aimed to assess the awareness of non-pharmacological interventions in neuropathic pain and to determine the attitude and practice towards the actual implementation of non-pharmacological modalities in attenuating pain symptoms of NP subjects. It was a Prospective, Observational study conducted on 100 OPD subjects of either gender aged 18-65 years with newly diagnosed chronic neuropathic pain conditions who agreed to participate in the study from January 2019 to June 2020 in the Pain Super-specialty clinic at Kempegowda Institute of Medical Sciences, Bangalore. Data were collected using a purposive sampling method, using a Self-administered, 14-item Questionnaire. The mean age of the study subjects was 44.68 years. 60.0% were aware of the existing...
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Posted by admin on Aug 31, 2022 in |
Doravirine, Lamivudine, and Tenofovir Disoproxil Fumarate belong to the class of anti-viral drugs and have been widely used in the treatment of HIV-I infection. Doravirine is a novel non-nucleoside reverse transcriptase inhibitor used in the combination of Lamivudine and Tenofovir Disoproxil Fumarate to effectively treat HIV/AIDS by overcoming the common resistance mutations without any possible drug interactions when co-administered. A literature review has reported few methods for the simultaneous estimation of Doravirine, Lamivudine, and Tenofovir Disoproxil Fumarate in combination with other antiviral drugs. The present investigation aimed to develop a simple, rapid, economical and stable indicating RP-HPLC method for the simultaneous estimation of Doravirine, Lamivudine and Tenofovir Disoproxil Fumarate in the pharmaceutical dosage form. The chromatographic separation was achieved on Kromasil C18 column with the dimensions of 250 x 4.6 mm, 5µm particle size, and maintained at 30 ˚C at a wavelength of 260nm. A combination of buffer (0.1% OPA) and Acetonitrile as mobile phase has optimized the method for efficient separation of drugs. Doravirine, Lamivudine and Tenofovir Disoproxil...
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Posted by admin on Aug 31, 2022 in |
Covid-19 associated mucormycosis rose sharply during India’s 2nd wave of coronavirus infections. The administration of immunosuppressive drugs led to increased susceptibility of patients to oppurtunistic diseases like mucormycosis. One of the causative species of mucormycosis is Rhizopus microsporus. For this study, we choose two chalcones and examined their ability to act as potential anti-mucormycosis agents by inhibiting the R. microsporus endo β-1,4-Mannanase protein. We studied their possibility to inhibit the SARS-CoV-2 main protease and RNA dependent RNA polymerase. The chalcones were docked against the proteins of interest using Autodock 4.0 followed by Molecular dynamics simulation. Our study revealed that 2’, 4’-dihydroxychalcone had the best docking with the endo β-1,4-Mannanase protein with steady root mean square deviation values and showed favourable docking with the SARS-CoV-2 proteins while passing all the drug likeliness filters. Thus 2’, 4’-dihydroxychalcone can be put through further verification to test its efficacy against the causative agents of mucormycosis and the Covid-19...
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Posted by admin on Aug 31, 2022 in |
The objective of the present investigation was to develop an alginate-HPMC K4M microbead-based gastro-retentive drug delivery system incorporating Famotidine for the treatment of gastrointestinal ulcers. The 32 full factorial designs were used for the optimization of famotidine-loaded floating microbeads. Two factors were evaluated, each at 3 levels; experimental trials were performed for all nine possible combinations. The amount of HPMC K4M (X1) and rotations of stirring speed (X2) were selected as independent variables. The % entrapment efficiency, % drug release at 12 hr, and % buoyancy were selected as dependent variables. The morphological properties, mean particle size, drug entrapment efficiency, drug loading, in-vitro buoyancy studies, in-vitro drug release, and in-vivo antiulcer activity of microbeads were all investigated. The effect of formulation variables on the response variables was statically evaluated by applying ANOVA at a 0.05 level using the software Design Expert® 13 (Stat-Ease, USA). The average size of optimized alginate-HPMC K4M microbeads was 0.86±0.35mm, with estimated entrapment effectiveness of 71.43± 0.21%, cumulative drug release of 98.75± 0.50% and percent...
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Posted by admin on Aug 31, 2022 in |
Inflammation is considered a primary physiologic defense mechanism that helps the body to protect itself against infection, burn, toxic chemicals, allergens, or other noxious stimuli. Inflammatory diseases are currently treated with steroidal and non-steroidal anti-inflammatory drugs (NSAIDs). Unfortunately, both of these widely prescribed drug classes have significant negative side effects. Hence, there is a need to develop new drugs with novel modes of action that do not produce considerable side effects. The use of traditional medicine and medicinal plants in most developing countries as a normative basis for the maintenance of good health, has been widely observed. So, crude flavonoid fraction of Mitragyna parvifolia was screened for anti-inflammatory activity using Carrageenan induced method and Formalin induced method at various doses (50,100 &200 mg/kg), and the results were compared with standard drug diclofenac sodium (20 mg/kg). It showed significant activity at 200 mg/kg. The Crude flavonoid fraction was also screened for antioxidant activity at various concentrations (20, 40, 60, 80,100μg/ml) using the DPPH free radical scavenging method. The antioxidant activity...
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