Posted by admin on Aug 31, 2019 in |
The study aimed to determine the relationship between nutritional status and nutrient intake and the incidence of anemia in adolescent girls in Kupang. It was an observational research with a cross-sectional design. The study was conducted at Kupang State Agricultural Polytechnic (KSAP) from December 2017 to January 2018. The research subjects were adolescent girls aged 16-19 years; withdrawal method was purposive sampling with a total of 57 subjects. The nutritional status was determined based on the BMI values, while the nutrient intake was calculated using a questionnaire. Hemoglobin levels were measured using a spectrophotometer. Data were descriptively analyzed. Pearson correlation test and Spearman’s Rho were to determine the relationship between parameters. The results showed 12.28% of subjects with poor nutritional status were anemic, 66.66% of normal nutritional status subjects had anemia and 10.53% of overweight subjects suffered from anemia. As many as 66.7% of the subjects had severe energy intake deficits, 43.9% subject had severe carbohydrate intake deficits, 50.9% of the subjects had severe protein intake deficits, and 70.2%...
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Posted by admin on Aug 31, 2019 in |
Traditional plant-based remedies continue to be an important therapeutic aid for treating many infections. In the current study, an attempt was made to know the antibacterial activity of different pod extracts of plants like Albizzia lebbeck, Cesalpina pulcherima, Leucenea leucocephala, & Tecoma stans against six different human pathogens E. coli, Enterobacter aerogenes, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, Proteus vulgaris. The pods were collected, washed, shade dried and powdered. The powders of the four different pods were extracted with methanol and water and screened for their phytochemical constituents by standard protocols and were shown to contain carbohydrates, flavonoids, tannins, phenols, saponins. All the extracts obtained were tested against the six pathogenic bacteria by cup plate method. Out of all the extracts, methanolic extracts of all the pods showed promising activity against all six pathogens. Methanolic extracts of C. pulcherima (2.2 cm) & T. stans (2.13 cm) exhibited greater activity than standard (2.0 cm) when tested against E. coli. The antibacterial activity of extracts may be linked to the presence...
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Posted by admin on Aug 31, 2019 in |
The present study was developed a suitable protocol for callus induction and plant regeneration of Cleome gynandra L. from leaf explants. Callus was initiated from leaf explants on Murashige and Skoog (MS) medium supplemented with different concentration and combinations of plant growth regulators. They are using (5-25 µM) of BAP (6-benzyladenine), KN (kinetin), and TDZ (thidiazuron) cytokinins along with (2-10 µM) different auxins [NAA (α-naphthalene acetic acid), IAA (indole-3 acetic acid), IBA (indole-3-butyric acid)]. Maximum regenerative callus (95%) was observed on TDZ + NAA (10 µM + 2 µM). Organogenic calli were transferred to shoot induction medium containing a different concentration of cytokinins (BAP, KIN, TDZ) ranging from 5-25 µM. Among the cytokinins tested BAP 10 µM produced number of shoots (35.2) with maximum shoot induction frequency (98%). Isolated shoots were carefully transferred to half-strength MS medium supplemented with auxins (IAA, IBA, and NAA) ranging from 2-10 µM. The maximum numbers of roots (8.8) were observed on 4 µM IBA. The rooted plantlets were successfully...
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Posted by admin on Aug 31, 2019 in |
Chromolaena and Lantana plants were analyzed to evaluate the existence of allelopathic effect using fully viable seeds of mung bean as the bioassay material. The study shows that pretreatment of mung bean seeds with various concentrations [1:1 and 1:2 (w/v)] of Chromolaena and Lantana leaf leachate for 24 h interval reduced the percentage germination. DNA and RNA contents were significantly reduced with a remarkable increase of amylase activity in seed samples pretreated with leaf leachate of Chromolaena and Lantana. The seedling performance was found to be much poor when the seedlings were raised from seeds which underwent pretreatment with the plant leachates. Evidenced from the lower levels of chlorophyll, DNA and RNA as well as a higher level of amylase activity in leaves of seedlings. Leaf leachate causes the reduction of germinability and influenced activity of amylase enzyme being the important allelopathic indices; it can be concluded that Chromolaena and Lantana can potentially render allelopathic action on mung bean. It further reemphasizes the fact that fast-growing exotic invasive weeds...
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Posted by admin on Aug 31, 2019 in |
The present investigation aims to evaluate the effect of methanol extract of Spatoglossum asperum for its anti-hyperglycemic activity against diabetics induced by alloxan in Wistar albino rats. Artificially diabetes was induced in albino rats by the administration through injection of a single dose of alloxan monohydrate (10 mg/kg, I.P). The 10 mg/kg methanol residue of S. asperum was administered as a single oral dose per day to diabetes-induced rats for 28 days. The outcome of algae residue feeding was estimated by various biochemical and hematological parameters such as RBC, WBC, Hb, and platelets and lipid profile. After feeding of algal methanol extract of S. asperum in diabetic rats for 28 days, the blood glucose had significantly decreased, whereas the liver glycogen level had increased. Histopathological examination of the pancreas was done. The methanol residues of the Spatoglossum asperum possess very effective anti-hyperglycemic activity on the diabetic rats as compared to...
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